Regulation of Atg5 and P62 during the follicular dysplasia process of newborn mice induced by DEHP

Wang Yuheng; Mu Xinyi; Su Yan; Xie Yi; Wang Yingxiong; He Junlin; Liu Xueqing; Chen Xuemei; Gao Rufei

doi:10.13406/j.cnki.cyxb.000881

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Regulation of Atg5 and P62 during the follicular dysplasia process of newborn mice induced by DEHP

更新时间：2024-01-17

- Regulation of Atg5 and P62 during the follicular dysplasia process of newborn mice induced by DEHP
- Journal of Chongqing Medical University Vol. 41, Issue 3, Pages: 223-227(2016)
- 作者机构：
  
  重庆医科大学公共卫生与管理学院生殖生物学研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000881
  CLC： R321
- Published：2016，
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[1]汪玉姮,穆欣艺,苏燕,谢翼,王应雄,何俊琳,刘学庆,陈雪梅,高茹菲.自噬相关蛋白Atg5及P62在DEHP引起新生鼠卵泡发育异常过程中的作用[J].重庆医科大学学报,2016,41(03):223-227.

Wang Yuheng, Mu Xinyi, Su Yan, et al. Regulation of Atg5 and P62 during the follicular dysplasia process of newborn mice induced by DEHP. [J]. Journal of Chongqing Medical University 41(3):223-227(2016)
[1]汪玉姮,穆欣艺,苏燕,谢翼,王应雄,何俊琳,刘学庆,陈雪梅,高茹菲.自噬相关蛋白Atg5及P62在DEHP引起新生鼠卵泡发育异常过程中的作用[J].重庆医科大学学报,2016,41(03):223-227. DOI： 10.13406/j.cnki.cyxb.000881.

Wang Yuheng, Mu Xinyi, Su Yan, et al. Regulation of Atg5 and P62 during the follicular dysplasia process of newborn mice induced by DEHP. [J]. Journal of Chongqing Medical University 41(3):223-227(2016) DOI： 10.13406/j.cnki.cyxb.000881.

摘要

目的:探讨自噬相关蛋白Atg5及P62在邻苯二甲酸二乙基己酯（diethylhexyl phthalate

DEHP）引起新生鼠卵巢卵泡发育异常这一过程中的作用。方法:将健康6

周龄清洁级成年雌性昆明鼠随机分为对照组（玉米油）和DEHP组

每组30只

均不做任何处理。成年雌鼠生产后的新生鼠分为对照组（玉米油）和DEHP组

采用腹腔注射方式

DEHP组按0.62 mg/kg浓度对新生鼠进行注射

对照组注射相同体积玉米油

从新生鼠出生第1天开始注射

每天1次

连续5 d

注射结束后收集新生鼠卵巢组织

HE染色观察卵巢各级卵泡的发育情况

q RT-PCR及Western blot检测卵泡发育标志分子GDF9及自噬相关蛋白Atg5、P62的表达情况。结果:与对照组比较

HE染色结果显示DEHP组原始卵泡数量明显减少（P=0.005）

而初级卵泡数量无明显改变（P=0.555）;q RT-PCR结果显示DEHP组新生鼠卵巢组织中卵泡发育标志分子GDF9 m RNA表达明显降低（P=0.034）

而自噬标志分子Atg5、P62 m RNA表达明显增加（P值分别为0.001、0.024）。Western blot结果显示DEHP组新生鼠卵巢中自噬相关蛋白Atg5及P62均明显增加

差异有统计学意义（P值分别为0.008、0.001）。结论:在DEHP暴露引起新生鼠卵巢卵泡发育异常这一过程中

自噬标记蛋白Atg5及P62可能发挥着重要作用。

Abstract

Objective:To investigate the effects of autophagy related protein Atg5 and P62 on the process of newborn mice follicular dysplasia caused by DEHP. Methods:Adult female Kun Ming mice of six to eight weeks at clean and healthy level were randomly divided into the control groups(corn oil)and the DEHP groups(30 in each group)without any treatment. The newborns were divided into the control groups(corn oil)and the DEHP groups. By intraperitoneal injection

the newborns of the DEHP groups were injected with DEHP at the concentration of 0.62 mg/kg

and the ones of the control groups were injected with the same volume of corn oil.The newborn mice were injected once a day from born to 5 days postpartum. Ovarian tissues of the newborn mice from two groups were collected. HE staining was used to observe the development of ovarian follicles at all levels. Real-time PCR and Western blot were employed to detect the expression of follicular developmental marker GDF9 and autophagy related proteins Atg5 and P62.Results:Compared with that of control groups

the number of primordial follicles of newborn mice from the DEHP groups decreased sig-nificantly

and there was no significant difference in the primary follicles number of the newborn mice between the two groups. The re-sults of Real-time PCR showed the levels of follicular developmental marker GDF9 decreased significantly

butautophagy related gene Atg5 and P62 m RNA in the newborn mice ovarian tissues from the DEHP group increased significantly. Western blot showed the expression of autophagy related proteins Atg5 and P62 in the newborn mice ovarian tissues were significantly increased following DEHP treatment. Conclusion:Atg5 and P62 might play an important role during the follicular dysplasia process of newborn mice induced by DEHP.

关键词

邻苯二甲酸二乙基己酯自噬卵泡发育Atg5P62

Keywords

diethylhexyl phthalateautophagyfollicular devel opmentAtg5P62

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Related Institution

Department of Endocrinology,The First Affiliated Hospital of Chongqing Medical University

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