Effect of pulsed high intensity focused ultrasound combined with microbubbles agent on apoptosis of VX2 tumors implanted in rabbits’ livers

Shi Lin; Liu Chunmei; Li Chongyan; Wang Qi; Xu Jie; Li Faqi

doi:10.13406/j.cnki.cyxb.000880

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Effect of pulsed high intensity focused ultrasound combined with microbubbles agent on apoptosis of VX2 tumors implanted in rabbits’ livers

更新时间：2024-01-17

- Effect of pulsed high intensity focused ultrasound combined with microbubbles agent on apoptosis of VX2 tumors implanted in rabbits’ livers
- Journal of Chongqing Medical University Vol. 41, Issue 10, Pages: 995-999(2016)
- 作者机构：
  
  重庆医科大学生物医学工程学院超声医学工程重庆市重点实验室省部共建超声医学工程国家重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000880
  CLC： R735.7;R454.3
- Published：2016，
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[1]史琳,刘春梅,李崇雁,王琦,徐杰,李发琪.脉冲高强度聚焦超声联合微泡介导兔肝VX2移植瘤细胞凋亡的研究[J].重庆医科大学学报,2016,41(10):995-999.

Shi Lin, Liu Chunmei, Li Chongyan, et al. Effect of pulsed high intensity focused ultrasound combined with microbubbles agent on apoptosis of VX2 tumors implanted in rabbits’ livers. [J]. Journal of Chongqing Medical University 41(10):995-999(2016)
[1]史琳,刘春梅,李崇雁,王琦,徐杰,李发琪.脉冲高强度聚焦超声联合微泡介导兔肝VX2移植瘤细胞凋亡的研究[J].重庆医科大学学报,2016,41(10):995-999. DOI： 10.13406/j.cnki.cyxb.000880.

Shi Lin, Liu Chunmei, Li Chongyan, et al. Effect of pulsed high intensity focused ultrasound combined with microbubbles agent on apoptosis of VX2 tumors implanted in rabbits’ livers. [J]. Journal of Chongqing Medical University 41(10):995-999(2016) DOI： 10.13406/j.cnki.cyxb.000880.

摘要

目的:探讨脉冲高强度聚焦超声（pulsed high intensity focused ultrasound

PHIFU）联合微泡（microbubbles

MBs）以非热效应导致兔肝VX2移植瘤细胞凋亡。方法:建立兔肝VX2移植瘤模型

将36只荷瘤兔随机分为对照组、PHIFU辐照组和PHIFU+MBs辐照组。PHIFU辐照参数为频率0.87 MHz、空间峰值声强（spatial peak intensity

ISP）5900 W/cm2、脉冲重复频率100 Hz、占空比15%、辐照时间90 s

MBs采用0.1 m L/kg Sono Vue。HIFU辐照后1 d处死动物

取部分肿瘤组织采用TUNEL法检测细胞凋亡

免疫组化染色检测PCNA抗原

剩余肿瘤组织进行2

5-氯化三苯基四氮唑（2

5-triphenyl tetrazolium chloride

TTC）染色观察。结果:对照组、PHIFU组和PHIFU+MBs组经TTC染色后

肉眼可见肿瘤组织被均匀红染

表明各组均无肉眼可见的凝固性坏死形成。TUNEL法检测发现

对照组发现少量阳性染色的肿瘤细胞

PHIFU组和PHIFU+MBs组见较多细胞核棕褐色染色的阳性细胞。PCNA检测结果表明

对照组靶区见大量着色部位在肿瘤细胞核的阳性细胞

而PHIFU组和PHIFU+UCA组阳性染色细胞少。PHIFU辐照组和PHIFU+UCA辐照组凋亡指数（apoptosis index

AI）比对照组高

增殖指数（proliferating index

PI）比对照组低（P=0.000）;PHIFU+MBs辐照组AI最高

PI最低（P=0.000）。结论:PHIFU联合MBs可促进兔肝VX2移植瘤细胞凋亡。

Abstract

Objective:To investigate the effect of pulsed high intensity focused ultrasound(PHIFU)combined with microbubbles(MBs)on apoptosis of VX2 tumors implanted in rabbits’ livers thnough non thermal conductivity. Methods:Liver VX2 tumor models were established in 36 rabbits

which were divided randomly into three groups:control group

PHIFU groups and PHIFU+MBs groups. PHIFU parameters used were as follows:ultrasound frequency 0.87 MHz

ISP(spatial peak intensity)5 900 W/cm2

pulse repetition frequency100 Hz

duty cycle 15%

and expose time 90 s. The rabbits were sacrificed one day after ultrasound exposure and the tumor tissues were taken. Apoptosis in situ was detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling(TUNEL);proliferating cell nuclear antigen expression(PCNA)was examined by immunohistochemical staining. The remaining tumor tissues were sent for 2

5-triphenyltetrazolium chloride(TTC)staining. Results:After HIFU exposure

liver tumors of all the three groups were uniformly stained red by TTC staining

suggesting that none of the animals had coagulated necrotic changes. TUNEL method detected a few positive staining tumor cells in control group;but in PHIFU group and PHIFU+MBs group

there were a lot of brow-colored positive apoptotic cells. PCNA detection showed more brow-colored positive cells in control group than in PHIFU group and PHIFU+MBs group. Apoptosis index(AI)of the tumor cells in the PHIFU group and PHIFU+MBs group was higher than that in the control group

while proliferating index(PI)of the tumor cells in PHIFU group and PHIFU+UCA group was lower than that in control group(P=0.000). AI of the tumor cells was the highest and PI was the lowest in PHIFU+UCA group(P=0.000). Conclusion:The non-thermal effects of PHIFU combined with MBs can promote apoptosis of VX2 tumors implanted in rabbits’ livers.

关键词

脉冲高强度聚焦超声微泡非热效应凋亡

Keywords

pulsed-high intensity focused ultrasoundultra sound contrast agentnonthermal effectsapoptosis

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