Preliminary study on the stability of hyaluronic acid-uricase multivesicular liposomes

Xie Jiangchuan; He Dan; Hu Xueyuan; Zhou Yunli; Yan Zijun; Chen Wei; Zhang Jingqing

doi:10.13406/j.cnki.cyxb.000879

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Preliminary study on the stability of hyaluronic acid-uricase multivesicular liposomes

更新时间：2024-01-17

- Preliminary study on the stability of hyaluronic acid-uricase multivesicular liposomes
- Journal of Chongqing Medical University Vol. 42, Issue 2, Pages: 191-196(2017)
- 作者机构：
  
  1. 重庆医科大学药学院重庆药物高校工程研究中心
  2. 重庆市中医院药剂科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000879
  CLC： R943
- Published：2017，
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Xie Jiangchuan, He Dan, Hu Xueyuan, et al. Preliminary study on the stability of hyaluronic acid-uricase multivesicular liposomes. [J]. Journal of Chongqing Medical University 42(2):191-196(2017)
DOI：

Xie Jiangchuan, He Dan, Hu Xueyuan, et al. Preliminary study on the stability of hyaluronic acid-uricase multivesicular liposomes. [J]. Journal of Chongqing Medical University 42(2):191-196(2017) DOI： 10.13406/j.cnki.cyxb.000879.

摘要

目的:制备了透明质酸修饰的尿酸酶多囊脂质体(hyaluronic acid-uricase liposomes

UHMVL)

并且对尿酸酶(uricase

UC)以及UHMVL在体外的活性和稳定性进行了初步的研究。方法:采用复乳法制备了UHMVL

并且测定了其包封率。然后从最适温度(n=3)、最适p H(n=3)、体外稳定性(n=3)初步考察了UHMVL和UC的差异。结果:UHMVL的包封率为(62.48±3.87)%。UHMVL及UC最适温度均为40℃;UHMVL的最适p H值为8.0

UC的最适p H值为8.5。稳定性测定结果表明

UHMVL体外稳定性均比UC好。UHMVL在提高了UC在体外活性的同时

也明显地增强了UC在体外的稳定性。结论:UHMVL在提高了UC体外活性的同时

也明显地增强了游离酶UC在体外的稳定性。

Abstract

Objective:To preparare hyaluronic acid-uricase liposomes（UHMVL）

and then to investigate the activity and stability difference between UHMVL and UC in vitro. Methods:UHMVL was prepared by double emulsion method. The optimum temperature（n=3）

optimum pH（n=3）

stability（n=3）on UC and UHMVL were primarily examined. Results:The encapsulation efficiency of UHMVL was（62.48±3.87）%. The optimum temperature of UHMVL was 40 ℃;the optimum pH of UHMVL was 8.0;the optimun pH of UC was 8.5. Stability of UHMVL was better than that of UC. UHMVL can improve the activity and enhances the stability of UC in vitro. Conclusion:UHLP can improve the activity

and significantly enhance the stability of free UC in vitro.

关键词

尿酸酶透明质酸多囊脂质体最适温度最适p H稳定性

Keywords

uricaseHyaluronic acidliposomesoptimum temperatureoptimum pHstabilities

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