Mechanism of Danshen drug-containing serum on TGF-β1 and CyclinD1 in hepatic stellate cells

Ye Feng; Cao Wenfu

doi:10.13406/j.cnki.cyxb.000876

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Mechanism of Danshen drug-containing serum on TGF-β1 and CyclinD1 in hepatic stellate cells

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- Mechanism of Danshen drug-containing serum on TGF-β1 and CyclinD1 in hepatic stellate cells
- Journal of Chongqing Medical University Vol. 41, Issue 4, Pages: 369-373(2016)
- 作者机构：
  
  重庆医科大学附属第一医院中西医结合科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000876
  CLC： R285.5
- Published：2016，
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[1]叶凤,曹文富.丹参含药血清调节大鼠肝星状细胞TGF-β1及细胞周期素CyclinD1的机制研究[J].重庆医科大学学报,2016,41(04):369-373.

Ye Feng, Cao Wenfu. Mechanism of Danshen drug-containing serum on TGF-β1 and CyclinD1 in hepatic stellate cells. [J]. Journal of Chongqing Medical University 41(4):369-373(2016)
[1]叶凤,曹文富.丹参含药血清调节大鼠肝星状细胞TGF-β1及细胞周期素CyclinD1的机制研究[J].重庆医科大学学报,2016,41(04):369-373. DOI： 10.13406/j.cnki.cyxb.000876.

Ye Feng, Cao Wenfu. Mechanism of Danshen drug-containing serum on TGF-β1 and CyclinD1 in hepatic stellate cells. [J]. Journal of Chongqing Medical University 41(4):369-373(2016) DOI： 10.13406/j.cnki.cyxb.000876.

摘要

目的:研究丹参含药血清调节大鼠肝星状细胞内转化生长因子β1（transforming growth factorβ1

TGF-β1）及细胞周期素CyclinD1的机制。方法:将20只Sprague-Dawley（SD）大鼠按照随机数字表法随机分为丹参低剂量组（1.04 g/kg）、中剂量组（2.08 g/kg）、高剂量组（4.17 g/kg）、空白组

并分别制备含药血清。体外传代培养肝星状细胞经10 ng/ml血小板衍生生长因子-BB（platelet-derived growth factor-BB

PDGF-BB）诱导后

再给予相应的含药血清干预

37℃培养24 h后收集细胞。经高效液相色谱法（HPLC）检测血清中丹参成分

q RT-PCR测定视黄酸受体-α（retinoic acid receptorα

RXR-α）及TGF-β1 mRNA

用Western blot法检测α-平滑肌肌动蛋白（α-smooth muscle actin

α-SMA）、Ⅰ型胶原蛋白（CollagenⅠ）及细胞周期素CyclinD1蛋白的表达情况。结果:丹参不同剂量处理组中

RXR-α mRNA表达量逐渐增加

而TGF-β1 mRNA、α-SMA蛋白、CollgenⅠ蛋白和CyclinD1蛋白表达逐渐降低

且与对照组比有统计学差异。结论:丹参含药血清可能通过促进RXR-α表达

抑制TGF-β1及CyclinD1表达

抑制肝星状细胞的增殖

进而抑制肝纤维化。

Abstract

Objective:To investigate the mechanism of Danshen drug-containing serum on transforming growth factor β1(TGF-β1)and CyclinD1 in hepatic stellate cells. Methods: According to the random number table

20 Sprague-Dawley(SD)rats were randomly divided into the Danshen(1.04 g/kg

2.08 g/kg

and 4.17 g/kg dosage)groups and normal control group. Blood was collected after gastric perfusion at 2 hours on 10 th day. The method of high performance liquid chromatography(HPLC)was used to detect the composition of Danshen in serum. HSCs were cultured in vitro and stimulated with platelet-derived growth factor-BB(PDGF-BB)

then were treated separately with the serum of Danshen(high

middle

and low dosage)

normal saline for 24 hours. After cells being collected

the expressions of retinoic acid receptor and TGF-β1 mRNA were measured by real-time PCR(q RT-PCR)

the content of α-smooth muscle actin(α-SMA)

typeⅠcollagen and CyclinD1 were detected by Western blot. Results:Compared with the normal control group

Danshen(low

middle

and high)groups could increase the expressions of retinoic acid receptor mRNA

and decrease the contents of TGF-β1 mRNA

α-SMA

type Ⅰ collagen and CyclinD1. Conclusion:Danshen may be achieved through increasing the expression of retinoic acid receptor and reducing TGF-β1 and CyclinD1 to inhibit the proliferation of HSCs

thus play a role of inhibition in liver fibrosis.

关键词

视黄酸受体转化生长因子-β1细胞周期素D1丹参含药血清肝星状细胞肝纤维化高效液相色谱法

Keywords

Retinoic acid receptortransforming growth factor β1(TGF-β1)CyclinD1Danshen drug-containing serumhepatic stel late cellshepatic fibrosishigh performance liquid chromato-graphy

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