Expression and significance of microtubule-associate protein 1B in rats with diffuse axonal injuries

Zhang Peng; Zhao Minzhu; Zhu Shisheng; Liu Meng; Gao Jun; Li Hongwei; Li Jianbo

doi:10.13406/j.cnki.cyxb.000852

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Expression and significance of microtubule-associate protein 1B in rats with diffuse axonal injuries

更新时间：2024-01-17

- Expression and significance of microtubule-associate protein 1B in rats with diffuse axonal injuries
- Journal of Chongqing Medical University Vol. 41, Issue 1, Pages: 34-37(2016)
- 作者机构：
  
  1. 重庆医科大学基础医学院法医学教研室
  2. 重庆医药高等专科学校医学技术院
  3. 重庆市公安局刑警总队技术处
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000852
  CLC： R651.15
- Published：2016，
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[1]张鹏,赵敏珠,朱士胜,刘梦,高军,李红卫,李剑波.微管相关蛋白-1B在弥漫性轴索损伤中的表达变化与意义[J].重庆医科大学学报,2016,41(01):34-37.

Zhang Peng, Zhao Minzhu, Zhu Shisheng, et al. Expression and significance of microtubule-associate protein 1B in rats with diffuse axonal injuries. [J]. Journal of Chongqing Medical University 41(1):34-37(2016)
[1]张鹏,赵敏珠,朱士胜,刘梦,高军,李红卫,李剑波.微管相关蛋白-1B在弥漫性轴索损伤中的表达变化与意义[J].重庆医科大学学报,2016,41(01):34-37. DOI： 10.13406/j.cnki.cyxb.000852.

Zhang Peng, Zhao Minzhu, Zhu Shisheng, et al. Expression and significance of microtubule-associate protein 1B in rats with diffuse axonal injuries. [J]. Journal of Chongqing Medical University 41(1):34-37(2016) DOI： 10.13406/j.cnki.cyxb.000852.

摘要

目的 :观察微管相关蛋白-1B（microtubule-associate protein 1B

MAP1B）在SD大鼠弥漫性轴索损伤（diffuse axonal injury

DAI）后脑组织中表达的变化

探索其表达变化的意义。方法 :采用改良的Marmarou法建立SD大鼠DAI模型

分为空白对照组（10只

不予任何处理）

假打击组（10只

给予致伤组相同的打击前准备

但不予以打击）

致伤6 h组（10只）

致伤1 d组（10只）和致伤3 d组（10只）

分别于打击6 h、1 d、3 d后

处死动物

提取脑组织

结合Western blot和免疫组化方法

观察SD大鼠脑组织中MAP1B表达的变化。结果:Western blot结果显示致伤6 h组（0.50±0.07）、致伤1 d组（0.56±0.07）和致伤3 d组（0.50±0.06）中MAP1B表达水平较空白对照组（0.30±0.06;P=0.001

P=0.000

P=0.001）、假打击组（0.30±0.04;P=0.001

P=0.000

P=0.001）明显升高。免疫组化结果显示致伤6 h组（4.70±0.79）、致伤1 d组（4.78±0.86）和致伤3 d组（4.70±0.89）中MAP1B表达水平较空白对照组（4.06±1.15;P=0.023

P=0.007

P=0.023）、假打击组（3.90±1.46;P=0.002

P=0.000

P=0.002）明显升高。结论:SD大鼠DAI后脑组织中MAP1B表达水平上调

提示其参与了DAI后轴索损伤的病理改变过程

并可能在稳定、修复及再生受损轴索的机制中起着重要作用。

Abstract

Objective:To examine the expression of the microtubule-associate protein 1B(MAP1B)in rats with diffuse axonal injury(DAI)as well as to elucidate the mechanisms of MAP1 B in the procession of axonal repairment. Methods:DAI was induced in adult male SD rats using an injury model adapted from Marmarou. The rats were divided into five groups: injured 6 h group(n=10)

injured1 d group(n=10)

injured 3 d group(n=10)

sham group(n=10)and sham-injured group(n=10). The animals were sacrificed at 6 h

on 1 d and 3 d post injury. Western blot and immunohistochemistry were performed to observe the expression of MAP1 B in rats with DAI. Results :Western blot and immunohistochemistry analyses showed MAP1 B expression was up-regulated in the brain tissues of injured 6 h group

injured 1 d group

injured 3 d group than in sham group and sham-injured group. Conclusion:Expression of MAP1 B is increased in the brain tissues of injury group

indicating that MAP1 B may be involved in the pathophysiological mechanisms of DAI and may play an important role in the repair of the axon after head trauma.

关键词

弥漫性轴索损伤微管相关蛋白-1B轴索修复SD大鼠

Keywords

diffuse axonal injurymicrotubule-associate protein 1BrepairmentSD rats

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Related Author

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Related Institution

Department of Forensic Medicine，College of Basic Medicine，Chongqing Medical University

Department of Biochemistry Faculty of Medical Technology，Chongqing Medical and Pharmaceutical College

Department of Pathology，Department of Basic Medicine，Zunyi Medical And Pharmaceutical College

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