Effects of chronic hypoxia and monocrotaline induced pulmonary hypertension on the expression of Ca<sup>2+</sup>-sensing receptor in rats

Xia Shihui; Du Mingcheng; Qin Tingjiang; Liu Yingbei; Wang Chaohui; An Yong

doi:10.13406/j.cnki.cyxb.000823

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Effects of chronic hypoxia and monocrotaline induced pulmonary hypertension on the expression of Ca²⁺-sensing receptor in rats

更新时间：2024-01-17

- Effects of chronic hypoxia and monocrotaline induced pulmonary hypertension on the expression of Ca²⁺-sensing receptor in rats
- Journal of Chongqing Medical University Vol. 41, Issue 9, Pages: 893-897(2016)
- 作者机构：
  
  重庆医科大学附属儿童医院心胸外科儿童发育疾病研究省部共建教育部重点实验室儿科学重庆市重点实验室重庆市儿童发育重大疾病诊治与预防国际科技合作基地
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000823
  CLC： R725.4
- Published：2016，
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[1]夏世辉,杜明成,秦廷江,刘盈贝,王朝辉,安永.慢性缺氧及野百合碱诱导大鼠肺动脉高压对钙敏感受体表达的影响[J].重庆医科大学学报,2016,41(09):893-897.

Xia Shihui, Du Mingcheng, Qin Tingjiang, et al. Effects of chronic hypoxia and monocrotaline induced pulmonary hypertension on the expression of Ca²⁺-sensing receptor in rats. [J]. Journal of Chongqing Medical University 41(9):893-897(2016)
[1]夏世辉,杜明成,秦廷江,刘盈贝,王朝辉,安永.慢性缺氧及野百合碱诱导大鼠肺动脉高压对钙敏感受体表达的影响[J].重庆医科大学学报,2016,41(09):893-897. DOI： 10.13406/j.cnki.cyxb.000823.

Xia Shihui, Du Mingcheng, Qin Tingjiang, et al. Effects of chronic hypoxia and monocrotaline induced pulmonary hypertension on the expression of Ca²⁺-sensing receptor in rats. [J]. Journal of Chongqing Medical University 41(9):893-897(2016) DOI： 10.13406/j.cnki.cyxb.000823.

摘要

目的:探讨慢性缺氧（chronic hypoxia

CH）及野百合碱（monocrotaline

MCT）诱导大鼠建立肺动脉高压（pulmonary hypertension

PH）形成差异及对肺动脉钙敏感受体（Ca2+-sensing receptor

Ca SR）表达影响。方法:分别通过CH及MCT诱导大鼠PH模型后检测实验组（缺氧3、7、14、21 d

MCT诱导3、7、14、21 d共8组）及对照（control

CON）组大鼠下列指标:右心导管法测大鼠肺动脉平均压力（mean pulmonary arterial pressure

m PAP）

右心室肥大指数（right ventricular hypertrophy index

RVHI）

取大鼠肺动脉以QPCR及Western blot方法检测Ca SR在肺动脉的表达情况情况。结果:CH组（CH 3、7、14、21 d）和MCT（3、7、14、21 d）大鼠m PAP、RVHI与CON组大鼠相比随干预时间延长（3、7、14、21 d）而升高

其中以CH 21d组和MCT 21 d组明显

而CH 21 d大鼠m PAP、RVHI均比MCT 21 d组低（P=0.000）;Ca SR m RNA表达在CH组和MCT组随着干预时间延长而逐渐升高

其中在CH 21 d和MCT 21 d的时候最高

均高于CON组（P=0.000

P=0.000）

而CH 21 d和MCT 21 d两者相比

MCT 21 d高于CH 21 d组

两者间有差异（P=0.000）;在CON组大鼠

CH各组及MCT各组大鼠肺动脉中均检测到Ca SR蛋白的表达

并随着干预时间延长

Ca SR蛋白趋势逐渐升高

以CH 21 d和MCT 21 d明显

而MCT 21 d组高于CH 21 d组。结论:相同时间内MCT诱导大鼠的肺动脉压高于CH建立PH模型

Ca SR m RNA及蛋白表达水平与肺动脉压力呈正相关

Ca SR参与了PH的形成及发展。

Abstract

Objective:To discuss the discrepancy of using chronic hypoxia(CH)and monocrotaline(MCT)to induce pulmonary hypertension(PH)and to explore their effects on Ca2+-sensing receptor(Ca SR). Methods:The male SD rats were randomly divided into control group(CON)

CH and MCT establishing pulmonary hypertension group. The rats in CON group were normally bred;the rats in CH group were placed in the glass box continued with 10% self-made oxygen for 21 d;the rats in MCT group were injected with 60mg/kg(BW)and were feed in SPF grade for 21 d. Mean pulmonary arterial pressure(m PAP)and right ventricular hypertrophy index(RVHI)were monitored by right heart catheterization. Expression of Ca SR m RNA and protein was detected by q PCR and Western blot. Results:Compared with that of CON group

m PAP and RVHI increased in CH and MCT group with the extending of intervening time

CH 21 d and MCT 21 d being the highest groups;m PAP and RVHI rats were significantly higher in MCT group than in CH group. Expressions of Ca SR m RNA in experimental 21 d group(CH and MCT)were significantly higher than that of CON group

higher in MCT 21 d group than in CH 21 d group. Expression of Ca SR protein increased in experimental group with the extending of intervening time

MCT 21 d being the highest group. Conclusion:Pulmonary artery pressure is higher in MCT group than in CH group in pulmonary hypertension rat model at the same time.Expressions of Ca SR m RNA and protein are positively correlated with the pulmonary artery pressure. Ca SR participates in the occurrence and development of PH.

关键词

慢性缺氧野百合碱肺动脉高压钙敏感受体

Keywords

chronic hypoxiamonocrotalinepulmonary hyper tensionCa2+-sensing receptor

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Related Institution

Department of Cardiology,Children’s Hospital of Chongqing Medical University

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Effects of chronic hypoxia and monocrotaline induced pulmonary hypertension on the expression of Ca2+-sensing receptor in rats

DOI：10.13406/j.cnki.cyxb.000823

摘要

Abstract

关键词

Keywords

references

Effects of chronic hypoxia and monocrotaline induced pulmonary hypertension on the expression of Ca²⁺-sensing receptor in rats