Objective:To determine the content of uridine and ganosine in Pinellia ternata（Thunb.）Breit. from different populations by HPLC. Methods:The Rhizoma pinelliae from different populations of southwest region was collected and the high-performance liquid chromatography（HPLC）was used to the content of the uridine and ganosine measurement. Hypersil ODS-C18（5 μm

4.6 ×150 mm）was used with aqueous solution containing 5% methanol as mobile phase

detection wavelength of 254 nm

flow rate of1 m L/min and tempemture of 25 ℃. Results:The method was developed with a good linearity with the range of 0.33 μg/m L-26 μg/m L

0.35 μg/m L-28 μg/m L for guanosine and uridine respectively. The order of guanosine and uridine contents in Rhizoma pinelliae from different places was Qingshui >Tianshui >Xihe >Nanchong >Dianjiang >Xichang >Shaodong >Weining >Shuicheng >Wenshan.Conclusion:The method is simple

accurate and can be used to control the internal quality of Rhizoma pinelliae medicine. From South to North and from east to west

the content of guanosine and uridine was increasing and was significantly different. It provides a new basis for assessing the quality of Rhizoma pinelliae of traditional Chinese medicine.