Effects of excessive expression of Caspase-3 on the apoptosis of olfactory receptor neurons

Xie Tianfei; Lou Weihua

doi:10.13406/j.cnki.cyxb.000796

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Effects of excessive expression of Caspase-3 on the apoptosis of olfactory receptor neurons

更新时间：2024-01-17

- Effects of excessive expression of Caspase-3 on the apoptosis of olfactory receptor neurons
- Journal of Chongqing Medical University Vol. 40, Issue 11, Pages: 1434-1437(2015)
- 作者机构：
  
  1. 河南医学高等专科学校耳鼻咽喉科
  2. 郑州大学第一附属医院耳鼻咽喉科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000796
  CLC： R765.41
- Published：2015，
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[1]谢天飞,娄卫华.嗅黏膜中Caspase-3的表达对嗅神经细胞凋亡的影响[J].重庆医科大学学报,2015,40(11):1434-1437.

Xie Tianfei, Lou Weihua. Effects of excessive expression of Caspase-3 on the apoptosis of olfactory receptor neurons. [J]. Journal of Chongqing Medical University 40(11):1434-1437(2015)
[1]谢天飞,娄卫华.嗅黏膜中Caspase-3的表达对嗅神经细胞凋亡的影响[J].重庆医科大学学报,2015,40(11):1434-1437. DOI： 10.13406/j.cnki.cyxb.000796.

Xie Tianfei, Lou Weihua. Effects of excessive expression of Caspase-3 on the apoptosis of olfactory receptor neurons. [J]. Journal of Chongqing Medical University 40(11):1434-1437(2015) DOI： 10.13406/j.cnki.cyxb.000796.

摘要

目的:探讨大鼠急性鼻窦炎时嗅黏膜中半胱氨酸天冬氨酸蛋白酶3（Caspase-3）的表达对嗅神经细胞（olfactory receptor neurons

ORNs）凋亡的影响。方法:将70只成年SD大鼠

按照配对设计

分成对照组和炎症组

造模后于不同时间取材

采用免疫组织化学方法检测嗅黏膜Caspase-3蛋白表达情况

原位细胞DNA断裂检测末端标记（TUNEL）法观察ORNs凋亡变化。结果:免疫组化结果

对照组ORNs中Caspase-3未见明显表达

炎症组ORNs中Caspase-3的表达在接种细菌后第2天开始升高

第5天明显升高（P<0.05）

第8天达到高峰（P<0.01）

第14天逐渐下降

第21天接近正常（P>0.05）。细菌接种后第2、21天

炎症组TUNEL阳性细胞计数与对照组相比没有统计学意义（P>0.05）

第5、8、14天差异有统计学意义（P<0.05）。结论:急性鼻窦炎时Caspase-3的过度表达可能是促进大鼠ORNs凋亡

导致嗅觉功能下降的原因之一。

Abstract

Objective:To explore the effects of excessive expression of Caspase-3 on the apoptosis of olfactory receptor neurons(ORNs).Methods:According to the matching design

70 adult SD rats were divided into two groups:control group and nasosinusitis group.The rats were sacrificed on 2 d

5 d

8 d

14 d and 21 d after inoculating bacterium. The tissues were analyzed for the expression of Caspase-3 by immunohistochemical method and the apoptosis by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL). Results:Caspase-3 immunoreactivity in nasosinusitis group began to increase on 2 d

increased clearly on 5 d(P<0.05)

peaked on 8 d(P<0.01)and began to decline on 14 d

closed to the normal on 21 d. On 2 d and 21 d in nasosinusitis group

a little TUNEL positive cell appeared in the olfactory mucosa

peaked on 8 d(P<0.05)and began to decline on 14 d. Conclusion:The expression of Caspase-3 may be a factor inducing the apoptosis of the ORNs.

关键词

急性鼻窦炎半胱氨酸天冬氨酸蛋白酶3嗅神经元细胞凋亡

Keywords

acute nasosinusitisCaspase-3olfactory receptor neuronscell apoptosis

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