Construction of SDF-1&amp;Fucoidan coatings to improve anti-coagulant and promote EPCs proliferation

Zhang Yan; Xue Guoneng; Wang Yan; Zhao Ansha; Ye Changrong; Su Hong

doi:10.13406/j.cnki.cyxb.000794

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Construction of SDF-1&Fucoidan coatings to improve anti-coagulant and promote EPCs proliferation

更新时间：2024-01-17

- Construction of SDF-1&Fucoidan coatings to improve anti-coagulant and promote EPCs proliferation
- Journal of Chongqing Medical University Vol. 40, Issue 11, Pages: 1465-1469(2015)
- 作者机构：
  
  1. 成都军区总医院血管内科
  2. 材料先进技术教育部重点实验室西南交通大学材料科学与工程学院
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000794
  CLC： R54
- Published：2015，
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[1]张彦,薛国能,王艳,赵安莎,叶长荣,苏红.SDF-1&Fucoidan涂层的抗凝血及促细胞生长研究[J].重庆医科大学学报,2015,40(11):1465-1469.

Zhang Yan, Xue Guoneng, Wang Yan, et al. Construction of SDF-1&Fucoidan coatings to improve anti-coagulant and promote EPCs proliferation. [J]. Journal of Chongqing Medical University 40(11):1465-1469(2015)
[1]张彦,薛国能,王艳,赵安莎,叶长荣,苏红.SDF-1&Fucoidan涂层的抗凝血及促细胞生长研究[J].重庆医科大学学报,2015,40(11):1465-1469. DOI： 10.13406/j.cnki.cyxb.000794.

Zhang Yan, Xue Guoneng, Wang Yan, et al. Construction of SDF-1&Fucoidan coatings to improve anti-coagulant and promote EPCs proliferation. [J]. Journal of Chongqing Medical University 40(11):1465-1469(2015) DOI： 10.13406/j.cnki.cyxb.000794.

摘要

目的:在材料表面构建SDF-1&Fucoidan复合功能涂层

调控内皮祖细胞（endothelia progenitor cells

EPCs）迁移、增殖和分化为内皮细胞（endothelia cells

ECs）参与血管的修复

解决临床植入材料应用中出现的再狭窄和晚期血栓。方法:在材料表面用硅烷试剂[（3-aminopropyl）triethoxysilane

APTE]进行处理

使表面富含氨基官能团

采用碳二亚胺盐酸盐（ethylcarbodiimide hydrochloride

EDC）和琥珀酰亚胺（N-Hydroxysuccinimide

NHS）催化偶联剂共接枝SDF-1和Fucoidan分子。材料学表征复合涂层的接枝结果和性能

细胞生物学实验评价涂层的抗凝性能和细胞的生长。结果:XPS结果表明SDF-1和Fucoidan分子成功被接枝到材料表面;血小板黏附SEM图像和统计数据证明SDF-1&Fucoidan涂层表面的血小板吸附数量明显减少

激活程度较低

且接枝Fucoidan浓度为100μg/ml时

抗凝效果达到最佳;EPCs培养结果表明SDF-1&Fucoidan涂层浓度在SDF-1为80 ng/ml与Fucoidan为100μg/ml时

细胞的黏附和生长状态最好。结论:SDF-1&Fucoidan涂层具备优异的抗凝血性以及明显促进EPCs的黏附与增殖。

Abstract

Objective:To construct SDF-1&Fucoidan bio-coatings to reduce restenosis and later thrombosis existing in implants materials application. Methods:The materials surface was firstly treated with APTE solution to form active amino-group;subsequently SDF-1 and Fucoidan were immobilized by a simply co-immobilization with EDC/NHS coupling agent. Various materials characterization and bio-compatibility evaluation were employed. Results:XPS results showed that SDF-1 and Fucoidan have been successfully grafted on materials surface. SEM image and statistics proved that platelet adhesion on SDF-1 & Fucoidan coatings was decreased and the activation was lowed. In particular

the optimal grafted Fucoidan concentration of the complex coating was about 100 μg/ml. Furthermore

endothelial progenitor cells(EPCs) culture experiment revealed the coating would promote EPCs adhesion and proliferation obviously;the optimal concentrations of SDF-1 and Fucoidan were respectively 80 ng/ml and 100 μg/ml. Conclusion:SDF-1 &Fucoidan complex coating displays excellent anti-coagulant

and promotes EPCs cell proliferation.

关键词

SDF-1Fucoidan内皮祖细胞抗凝血

Keywords

SDF-1Fucoidanendothelial progenitor cellsanti-coagulant

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Construction of SDF-1&Fucoidan coatings to improve anti-coagulant and promote EPCs proliferation

DOI：10.13406/j.cnki.cyxb.000794

摘要

Abstract

关键词

Keywords

references