Effect of Curcumin on migration of human umbilical vein endothelial cell induced by oxidized low-density lipoprotein

Cao Li; Zhang Dongying; Liu Jian

doi:10.13406/j.cnki.cyxb.000778

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Effect of Curcumin on migration of human umbilical vein endothelial cell induced by oxidized low-density lipoprotein

更新时间：2024-01-17

- Effect of Curcumin on migration of human umbilical vein endothelial cell induced by oxidized low-density lipoprotein
- Journal of Chongqing Medical University Vol. 40, Issue 11, Pages: 1470-1475(2015)
- 作者机构：
  
  重庆医科大学附属第一医院心内科、眼科学重庆市市级重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000778
  CLC： R285
- Published：2015，
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[1]曹黎,张冬颖,刘剑.姜黄素对氧化性低密度脂蛋白诱导的内皮细胞迁移能力的影响[J].重庆医科大学学报,2015,40(11):1470-1475.

Cao Li, Zhang Dongying, Liu Jian. Effect of Curcumin on migration of human umbilical vein endothelial cell induced by oxidized low-density lipoprotein. [J]. Journal of Chongqing Medical University 40(11):1470-1475(2015)
[1]曹黎,张冬颖,刘剑.姜黄素对氧化性低密度脂蛋白诱导的内皮细胞迁移能力的影响[J].重庆医科大学学报,2015,40(11):1470-1475. DOI： 10.13406/j.cnki.cyxb.000778.

Cao Li, Zhang Dongying, Liu Jian. Effect of Curcumin on migration of human umbilical vein endothelial cell induced by oxidized low-density lipoprotein. [J]. Journal of Chongqing Medical University 40(11):1470-1475(2015) DOI： 10.13406/j.cnki.cyxb.000778.

摘要

目的:研究姜黄素（Curcumin）对氧化性低密度脂蛋白（oxidized low-density lipoprotein

ox-LDL）诱导的内皮细胞迁移能力的影响

并探讨其可能的机制。方法:体外培养人脐静脉内皮细胞（human umbilical vein endothelial cells

HUVECs）

不同浓度的ox-LDL处理HUVECs 24 h后

流式细胞仪检测细胞凋亡率。实验分为正常对照组、ox-LDL组、Crcumin预处理+ox-LDL组。激光共聚焦检测氧自由基（reactive oxygen species

ROS）;酶标仪法测一氧化氮（nitric oxide

NO）;Westren blot测定一氧化氮合酶（endothelial nitric oxide synthase

e NOS）、磷酸化一氧化氮合酶（ser1177位点）（phosphorylated e NOSS1177

p-e NOSS1177）、蛋白激酶B（protein kinase B

Akt）、磷酸化蛋白激酶B（Ser473位点）（phosphorylated Akt S473

p-Akt S473）表达水平;细胞迁移实验检测HUVECs迁移能力。结果:高浓度的ox-LDL促进HUVECs凋亡（P=0.010）。与对照组比较

ox-LDL促进ROS生成（P=0.001）

降低NO含量（P=0.000）

下调p-Akt S473、p-e NOSS1177蛋白表达（P=0.000

P=0.000）

抑制了HUVECs迁移能力（P<0.05）;与ox-LDL组比较

Curcumin预处理能够显著地降低ROS水平（P<0.05）

增加p-Akt S473、p-e NOSS1177蛋白表达（P<0.05

P<0.05）

提高NO水平（P<0.05）

并且增加细胞迁移能力（P<0.05）。结论:姜黄素抑制ox-LDL诱导的ROS生成

可能通过Akt/e NOS/NO通路改善了内皮细胞的迁移能力。

Abstract

Objective:To investigate the effect of Curcumin on the migration of human umbilical vein endothelial cells(HUVECs)induced by oxidized low-density lipoprotein(ox-LDL). Methods:The HUVECs were cultured in vitro and treated with ox-LDL in different concentrations for 24 h;the apoptosis rate was determined by flow cytometry. Cells were divided into three groups: normal control group;ox-LDL treatment group;curcumin pretreatment+ox-LDL group. The concentration of reactive oxygen species(ROS) was measured by laser confocal microscopy;the level of nitric oxide(NO)was dectected by method of colorimetry;the protein levels of endothelial nitric oxide synthase(e NOS)

phosphorylated e NOSS1177(p-e NOSS1177)

protein kinase B(Akt) and phosphorylated Akt S473(p-AktS473)were detected by Western blot;the migration ability of endothelial cells was measured by transwell method. Results:High concentrations(≥40 mg/L)of ox-LDL increased the apoptosis rate significantly(P=0.010). Compared with those of control group

the level of ROS(P=0.001)was obviously increased by ox-LDL

phosphorylation of Akt S473

e NOSS1177

concentration of NO and the migration ability significantly were inhibited(P=0.000

P=0.000

P=0.000). Compared with those of ox-LDL treatment group

the generation of ROS(P<0.05)was reduced by curcumin pretreatment

and the phosphorylation of Akt S473

e NOSS1177

the concentration of NO(P<0.05

P<0.05

P<0.05)and the ability of migration were increased significantly(P<0.05). Conclusion:Curcumin inhibits the production of ROS induced by ox-LDL

and may increase the phosphorylation of Akt S473 and e NOSS1177 to promote the ability of endothelial cell migration.

关键词

氧化性低密度脂蛋白人脐静脉内皮细胞细胞迁移姜黄素

Keywords

oxidized low-density lipoproteinhuman umbilical vein endothelial cells(HUVECs)cell migrationcurcumin

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