Effects of benzo(a) pyrene exposure on morphology of the nervous system and its mechanism

Wei Ke; Tang Yan; Luo Long; Duan Li; Qing Qizhong; Zhou Li; Tu Baijie

doi:10.13406/j.cnki.cyxb.000655

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Effects of benzo(a) pyrene exposure on morphology of the nervous system and its mechanism

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- Effects of benzo(a) pyrene exposure on morphology of the nervous system and its mechanism
- Journal of Chongqing Medical University Vol. 41, Issue 1, Pages: 23-28(2016)
- 作者机构：
  
  1. 重庆医科大学公共卫生与管理学院劳动与环境卫生教研室
  2. 四川医科大学公共卫生学院劳动与环境卫生教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000655
  CLC： R114
- Published：2016，
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[1]魏科,汤艳,罗珑,段利,秦启忠,周莉,涂白杰.B[a]P致SD幼鼠神经组织形态学损伤及其机制研究[J].重庆医科大学学报,2016,41(01):23-28.

Wei Ke, Tang Yan, Luo Long, et al. Effects of benzo(a) pyrene exposure on morphology of the nervous system and its mechanism. [J]. Journal of Chongqing Medical University 41(1):23-28(2016)
[1]魏科,汤艳,罗珑,段利,秦启忠,周莉,涂白杰.B[a]P致SD幼鼠神经组织形态学损伤及其机制研究[J].重庆医科大学学报,2016,41(01):23-28. DOI： 10.13406/j.cnki.cyxb.000655.

Wei Ke, Tang Yan, Luo Long, et al. Effects of benzo(a) pyrene exposure on morphology of the nervous system and its mechanism. [J]. Journal of Chongqing Medical University 41(1):23-28(2016) DOI： 10.13406/j.cnki.cyxb.000655.

摘要

目的 :通过观察苯并[a]芘（Benzo（a）pyrene

B[a]

P）对新生SD大鼠脑组织形态学、超微病理学和生化指标的改变

探讨B[a]P神经发育毒性的分子机制。方法 :将48只新生雄性SD大鼠随机分为溶剂对照组、低剂量组、中剂量组、高剂量组

每组各12只

从出生后第5天始

每天灌胃1次

持续14 d。染毒后制切片

光镜、电镜观察海马形态学改变

化学比色法测定生化指标变化。结果:与溶剂组比较

染毒组幼鼠可见不同程度的脑组织损伤;海马组织超氧化物歧化酶含量、Na

-K

-ATP酶和Ca

-Mg

-ATP酶的活性明显下降

丙二醛含量明显增高

差异均具有统计学意义（P

0.05）。结论 :B[a]P可致新生SD大鼠神经发育毒性

其机制可能与海马组织氧化应激产生、Na

-K

-ATP酶和Ca

-Mg

-ATP酶活性下降有关。

Abstract

Objective:To explore the relationship between Benzo（a）pyrene（B[a]P）exposure and the changes of morphology

oxidative stress and ATPase in hippocampus in newly born SD rats

and to examine the molecular mechanism of developmental neurotoxicity caused by B[a]

P. Methods:Forty eight male new born SD rats were randomly assigned to one of the following four groups（n=12/group）:vehicle control group

0.02

0.2

2 mg/kg of B[a]P groups. The rats were given oral dose of B[a]

P once a day for 14 days starting from the fifth day after birth. After infection period

optical slices and electron-microscope slices from brain（cortex

hippocampi

cerebellum and brain stem）were made to observe. The colorimetric technique was used to detect the activity of SOD

MDA

Na+-K+-ATPase and Ca

-Mg

-ATP in hippocampus. Results:Brain tissue damage at various degrees was observed in the B[a]P groups compared with that of control group. SOD content

-K

-ATPase and Ca

-Mg

-ATPase of B[a]P groups decreased obviously compared with those of control group

and MDA content of B[a]

P groups increased obviously

with statistically significant differences（P

0.05）. Conclusion:B[a]P could lead to developmental neurotoxicity of new born SD rats

and the change of oxidative stress

the decreased ATPase may play an important role in B[a]

P-caused developmental neurotoxicity.

关键词

苯并[a]芘神经发育毒性形态学氧化应激三磷酸腺苷酶

Keywords

Benzo(a)pyrenedevelopmental neurotoxicitymorphologyoxidative stressATPase

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