Effects of renal denervation on atherosclerosis in rabbits and its mechanism

Yang Hanxuan; Tan Zhen; Lu Jiayi; Mei Xia; Yin Yuehui

doi:10.13406/j.cnki.cyxb.000626

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Effects of renal denervation on atherosclerosis in rabbits and its mechanism

更新时间：2024-01-17

- Effects of renal denervation on atherosclerosis in rabbits and its mechanism
- Journal of Chongqing Medical University Vol. 41, Issue 4, Pages: 325-331(2016)
- 作者机构：
  
  1. 重庆医科大学附属第二医院心内科
  2. 重庆市中山医院心内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000626
  CLC： R543.5
- Published：2016，
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[1]杨瀚晅,谭震,卢嘉奕,梅霞,殷跃辉.肾脏去神经对家兔动脉粥样硬化的影响及其机制研究[J].重庆医科大学学报,2016,41(04):325-331.

Yang Hanxuan, Tan Zhen, Lu Jiayi, et al. Effects of renal denervation on atherosclerosis in rabbits and its mechanism. [J]. Journal of Chongqing Medical University 41(4):325-331(2016)
[1]杨瀚晅,谭震,卢嘉奕,梅霞,殷跃辉.肾脏去神经对家兔动脉粥样硬化的影响及其机制研究[J].重庆医科大学学报,2016,41(04):325-331. DOI： 10.13406/j.cnki.cyxb.000626.

Yang Hanxuan, Tan Zhen, Lu Jiayi, et al. Effects of renal denervation on atherosclerosis in rabbits and its mechanism. [J]. Journal of Chongqing Medical University 41(4):325-331(2016) DOI： 10.13406/j.cnki.cyxb.000626.

摘要

目的:探讨肾脏去神经对家兔动脉粥样硬化影响及其机制。方法:28只雄性新西兰白兔随机平均分为正常对照组（control）、肾脏去神经（renal denervation

RDN）+高脂组、假手术（sham）+高脂组、单纯高脂饮食（high-fat-diet

HFD）组。检测血脂、血浆去甲肾上腺素（norepinephrine

NE）和氧化低密度脂蛋白（oxidized low density lipoprotein

ox-LDL）浓度

免疫组化检测血管紧张素Ⅱ（angiotensinⅡ

AngⅡ）、单核细胞趋化蛋白-1（monocyte chemoattractant protein-1

MCP-1）和血小板内皮细胞粘附分子-1（platelet endothelial cell adhesion molecule-1

PECAM-1）的表达;Western blot检测细胞间粘附分子-1（intercellular cell adhesion molecule

ICAM-1）和血管细胞间粘附分子-1（vascular cell adhesion molecule

VCAM-1）的表达;RT-PCR检测MCP-1、PECAM-1、ICAM-1和VCAM-1的m RNA的表达。结果:术后RDN组NE水平低于sham组和HFD组（P<0.05）

其血浆甘油三酯（triglyceride

TG）浓度低于HFD组（P<0.05）;RDN组血浆ox-LDL浓度、主动脉的AngⅡ和粘附分子表达较sham组和HFD组明显降低（P<0.05）

RDN组内膜/中膜比值较HFD组降低（P<0.05）。结论:RDN可降低TG浓度

同时可能通过降低交感神经活性

下调粘附分子的表达

延缓动脉粥样硬化形成。

Abstract

Objectives:To evaluate the effects of the renal denervation on atherosclerosis in rabbits and its mechanism. Methods:Twentyeight male New Zealand White rabbits were randomly divided into control group

renal denervation(RDN)+high-fat-diet(HFD)group

Sham+HFD group and HFD group. Blood samples were collected to measure norepinephrine(NE)

lipids and oxidized low density lipoprotein(ox-LDL)levels. Angiotensin Ⅱ(Ang Ⅱ)

monocyte chemotactic protein-1(MCP-1)and platelet endothelial cell adhesion molecule-1(PECAM-1)were detected by immunohistochemistry. Intercellular adhesion molecule-1(ICAM-1)and vascular intercellular adhesion molecules-1(VCAM-1)were detected by Western bolt and the m RNA expressions of MCP-1

ICAM-1

VCAM-1 and PECAM-1 were detected by RT-PCR. Results:The NE level was lower in RDN group than in sham+HFD groups after RDN procedure.TG was lower in RDN group than in HFD group. Plasma ox-LDL was significantly decreased in RDN+HFD group compared with that in HFD group and Sham+HFD group(P<0.05). Meanwhile

the expressions of AngⅡ

MCP-1

ICAM-1

VCAM-1 and PECAM-1 were significantly down-regulated in RDN+HFD group(P<0.05). The ratio of intima-media thickness in aorta was significantly decreased in RDN group compared with those in HFD group(P<0.05). Conclusion:Renal denervation could decrease the level of TG and may down-regulate the expression of adhesion molecules by decreasing sympatholytic activity

so it may delay atherosclerosis in cholesterol-fed rabbits.

关键词

动脉粥样硬化肾脏去神经血管紧张素Ⅱ粘附分子

Keywords

atherosclerosisrenal denervationangiotensinⅡAdhesion molecules

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