Clinical observation of amniotic membrane transplantation combined with entropion correction for ocular surface and eyelid burns

LIU Wen-hui; XU Shuo; PAN Ying; GU Yan; SUN Hong-juan; ZOU Wen-jun

doi:10.11659/jjssx.12E022090

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Clinical observation of amniotic membrane transplantation combined with entropion correction for ocular surface and eyelid burns

Clinical Research | 更新时间：2023-12-13

- Clinical observation of amniotic membrane transplantation combined with entropion correction for ocular surface and eyelid burns
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 12, Pages: 1072-1075(2023)
- 作者机构：
  
  江南大学附属中心医院/无锡市第二人民医院眼科，江苏无锡 214000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.12E022090
  CLC： R779.6;R644
- Published：25 December 2023，
  
  Received：18 December 2022，
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刘文慧,徐烁,潘莹等.羊膜移植联合睑内翻矫正术治疗眼表及眼睑烧伤的临床观察[J].局解手术学杂志,2023,32(12):1072-1075.

LIU Wen-hui,XU Shuo,PAN Ying,et al.Clinical observation of amniotic membrane transplantation combined with entropion correction for ocular surface and eyelid burns[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(12):1072-1075.
刘文慧,徐烁,潘莹等.羊膜移植联合睑内翻矫正术治疗眼表及眼睑烧伤的临床观察[J].局解手术学杂志,2023,32(12):1072-1075. DOI： 10.11659/jjssx.12E022090.

LIU Wen-hui,XU Shuo,PAN Ying,et al.Clinical observation of amniotic membrane transplantation combined with entropion correction for ocular surface and eyelid burns[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(12):1072-1075. DOI： 10.11659/jjssx.12E022090.

摘要

目的

探讨羊膜移植联合睑内翻矫正术治疗眼表及眼睑烧伤患者的临床效果。

方法

选取于我院行手术治疗的化学或热烧伤致眼表损伤及下眼睑内翻的80例患者为研究对象，所有患者均接受羊膜移植联合睑内翻矫正术治疗。患者于术前、术后8周分别进行裂隙灯显微镜检查并拍照；比较患者术前、术后8周视力变化情况、泪膜破裂时间以及血清血管内皮生长因子（VEGF）、转化生长因子β1（TGF-β1）、胰岛素生长因子1（IGF-1）水平；术后8周，采集中央角膜和角膜缘的脱落上皮细胞，采用PAS染色及免疫荧光染色进行分析。

结果

术后8周，患者眼表状况恢复良好，眼睑内翻成功矫正，患者视力显著优于术前（

0.05）。与术前比较，术后8周患者泪膜破裂时间显著延长（

0.05），血清VEGF、TGF-β1、IGF-1水平显著升高（

0.05）。PAS染色显示中央角膜及角膜缘无杯状细胞；免疫荧光染色显示细胞角蛋白12（CK12）在角膜上皮细胞中高表达，在角膜缘上皮靠近角膜侧的细胞中低表达。

结论

羊膜移植联合睑内翻矫正术治疗眼部烧伤效果显著，可促进患者眼表修复，矫正眼睑内翻，改善术后视力，实现角膜干细胞的自体扩增，是一种安全有效的治疗方法。

Abstract

Objective

To explore the clinical effect of amniotic membrane transplantation combined with entropion correction for

ocular surface and eyelid burns.

Methods

A total of 80 patients with ocular surface injury and lower eyelid entropion caused by chemical or thermal burns who received surgical treatment in our hospital were selected as the research objects. All patients received amniotic membrane transplantation combined with entropion correction. All patients were examined by slit-lamp microscope and photographed before and

8 weeks after surgery. The visual acuity changes， tear film rupture time and serum levels of vascular endothelial growth factor （VEGF）， transforming growth factor β1 （TGF-β1） and insulin growth factor 1 （IGF-1） were compared before and 8 weeks after surgery.Eight weeks after surgery， exfoliated epithelial cells of the central cornea and limbus were collected and analyzed by PAS staining and immunofluorescence staining.

Results

At 8 weeks after surgery， the patient's ocular surface condition recovered well， eyelid entropion was successfully

corrected， postoperative visual acuity was significantly better than that before surgery （

0.05）. Compared with before surgery， the tear film rupture time 8 weeks after surgery was significantly prolonged （

0.05）， and the serum VEGF， TGF-β1 and IGF-1 levels 8 weeks

after surgery were significantly increased （

0.05）. PAS staining showed that there was no goblet cell in the central cornea and limbus.

Immunofluorescence staining showed that cytokeratin 12 （CK12） was highly expressed in the corneal epithelial cells， and low expressed in corneal limbal epithelial cells near the cornea.

Conclusion

Amniotic membrane transplantation combined with entropion correction has a significant therapeutic effect on patients with ocular burn， which can promote ocular surface repair， correct entropion， improve postoperative visual acuity， and achieve self-amplification of cornea stem cells. It is a safe and effective treatment method.

关键词

羊膜移植睑内翻矫正术眼表烧伤上皮细胞

Keywords

amniotic membrane transplantationentropion correctionocular surface burnepithelial cells

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