Relationship between the expression of TFEB and TREM-1 in peripheral blood of patients with pancreatitis after endoscopic retrograde cholangiopancreatography and their condition

CHEN Zhao-wei; KANG Li-li; SONG Jian; ZHAO Xin-kai

doi:10.11659/jjssx.12E022080

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Relationship between the expression of TFEB and TREM-1 in peripheral blood of patients with pancreatitis after endoscopic retrograde cholangiopancreatography and their condition

Clinical Research | 更新时间：2023-07-31

- Relationship between the expression of TFEB and TREM-1 in peripheral blood of patients with pancreatitis after endoscopic retrograde cholangiopancreatography and their condition
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 8, Pages: 692-695(2023)
- 作者机构：
  
  海南省肿瘤医院消化内镜科，海南海口 570100
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.12E022080
  CLC： R576
- Published：25 August 2023，
  
  Received：15 December 2022，
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陈昭伟,康丽丽,宋健等.经内镜逆行胰胆管造影术后胰腺炎患者外周血TFEB、TREM-1表达与病情的关系[J].局解手术学杂志,2023,32(08):692-695.

CHEN Zhao-wei,KANG Li-li,SONG Jian,et al.Relationship between the expression of TFEB and TREM-1 in peripheral blood of patients with pancreatitis after endoscopic retrograde cholangiopancreatography and their condition[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(08):692-695.
陈昭伟,康丽丽,宋健等.经内镜逆行胰胆管造影术后胰腺炎患者外周血TFEB、TREM-1表达与病情的关系[J].局解手术学杂志,2023,32(08):692-695. DOI： 10.11659/jjssx.12E022080.

CHEN Zhao-wei,KANG Li-li,SONG Jian,et al.Relationship between the expression of TFEB and TREM-1 in peripheral blood of patients with pancreatitis after endoscopic retrograde cholangiopancreatography and their condition[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(08):692-695. DOI： 10.11659/jjssx.12E022080.

摘要

目的

探讨行内镜逆行胰胆管造影术（ERCP）后胰腺炎（PEP）患者外周血单个核细胞中转录因子EB（TFEB）、髓样细胞触发受体-1（TREM-1）表达与病情程度的关系。

方法

选取2019年6月至2022年3月在本院就诊的PEP患者105例为观察对象，设为PEP组，根据患者病情严重程度将其分为轻度组（62例）、中度组（32例）和重度组（11例）；选择同期ERCP后未发生PEP患者110例作为对照组。实时荧光定量PCR法检测患者手术前后外周血单个核细胞中TFEB、TREM-1表达水平；采用免疫比浊法检测手术前后血清C反应蛋白（CRP）水平；采用ELISA法检测血清白细胞介素-1（IL-1）、白细胞介素-6（IL-6）水平。术后对患者进行急性生理健康与慢性疾病（APACHE Ⅱ）评分；

Spearman

法分析PEP患者外周血TFEB、TREM-1表达水平与APACHE Ⅱ评分的相关性；

Pearson

法分析PEP患者外周血TFEB、TREM-1表达水平与CRP、IL-1、IL-6的相关性。

结果

PEP组术后血清CRP、IL-1、IL-6水平显著高于术前，且PEP组高于对照组（

0.05）。与术前比较，PEP组术后外周血TFEB表达水平显著降低，TREM-1表达水平显著升高（

0.05）；与对照组比较，PEP组术后外周血TFEB表达水平显著降低，TREM-1表达水平显著升高（

0.05）。与轻度组比较，中度组、重度组PEP患者术后外周血TFEB表达水平显著降低，TREM-1表达水平及APACHEⅡ评分显著升高（

0.05）；与中度组比较，重度组PEP患者术后外周血TFEB表达水平显著降低，TREM-1表达水平及APACHEⅡ评分显著升高（

0.05）。相关性分析结果显示，外周血TFEB表达水平与APACHEⅡ评分、血清CRP、IL-1、IL-6水平呈负相关（

0.05），外周血TREM-1表达水平与APACHEⅡ评分、血清CRP、IL-1、IL-6水平呈正相关（

0.05）。

结论

PEP患者外周血单个核细胞中TFEB表达水平降低，TREM-1表达水平升高，TFEB、TREM-1与患者病情严重程度均相关，可用于评估PEP病情。

Abstract

Objective

To investigate the relationship between the expression of transcription factor EB （TFEB） and triggering receptor expressed on myeloid cells-1 （TREM-1） in peripheral blood mononuclear cells of patients with pancreatitis （PEP） after endoscopic retrograde cholangiopancreatography （ERCP） and the severity of the disease.

Methods

A total of 105 PEP patients who were treated in our hospital from June 2019 to March 2022 were selected as the observation objects and set as the PEP group. According to the severity of the patients' conditions， they were divided into the mild group （62 cases）， the moderate group （32 cases） and the severe group （11 cases）. A total of 110 patients without PEP after ERCP during the same period were selected as the control group. The expression levels of TFEB and TREM-1 in peripheral blood mononuclear cells before and after surgery were detected by Real-time fluorescence quantitative PCR. The

levels of serum C-reactive protein （CRP） before and after surgery were detected by immunoturbidimetry. The levels of serum interleukin-1 （IL-1））and interleukin-6 （IL-6） were detected by ELISA. The acute physiology and chronic health evaluation Ⅱ （APACHE Ⅱ） score was performed on the patients after operation.

Spearman

method was used to analyze the correlation between the expression levels of TFEB and TREM-1 in peripheral blood of PEP patients and the APACHE Ⅱ score.

Pearson

method was used to analyze the correlation between the expression levels of TFEB and TREM-1 in peripheral blood of PEP patients and CRP， IL-1 and IL-6.

Results

The levels of serum CRP， IL-1 and IL-6 after operation in the PEP group were significantly higher than those before operation， and those in the PEP group were higher than those in the control group （

0.05）. Compared with before operation， the expression level of TFEB in peripheral blood after operation in the PEP group was significantly decreased， and the expression level of TREM-1 was significantly increased （

0.05）. Compared with the control group， the expression level of TFEB in peripheral blood after operation in the PEP group was significantly decreased， while the expression level of TREM-1 was significantly increased （

0.05）. Compared with the mild group， the expression levels of TFEB in peripheral blood of PEP patients after operation in the moderate group and the severe group were significantly decreased， while the expression levels of TREM-1 and APACHE Ⅱ score were significantly increased （

0.05）. Compared with the moderate group， the expression level of TFEB in peripheral blood of PEP patients after operation in the severe group was significantly decreased， while the expression level of TREM-1 and APACHE Ⅱ score were significantly increased （

0.05）. The results of correlation analysis showed that the expression level of TFEB in peripheral blood was negatively correlated with APACHE Ⅱ score， serum CRP， IL-1 and IL-6 levels （

0.05）， while the expression level of TREM-1 in peripheral blood was positively correlated with APACHE Ⅱ score， serum CRP， IL-1 and IL-6 levels （

0.05）.

Conclusion

The expression level of TFEB in peripheral blood mononuclear cells of PEP patients is decreased， while the expression level of TREM-1 is increased. TFEB and TREM-1 are related to the severity of the patients' disease， which can be used to evaluate the condition of PEP.

关键词

内镜逆行胰胆管造影术胰腺炎转录因子EB髓样细胞触发受体-1外周血单个核细胞

Keywords

endoscopic retrograde cholangiopancreatographypancreatitistranscription factor EBtriggering receptor expressed on myeloid cells-1peripheral blood mononuclear cells

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