Expression of serum miR-384 and LIN28B in diabetic retinopathy and their diagnostic value

TANG Yun-hu; ZHAO Yuan; DAI Yan

doi:10.11659/jjssx.12E022014

您当前的位置：

首页 >

文章列表页 >

Expression of serum miR-384 and LIN28B in diabetic retinopathy and their diagnostic value

Clinical Research | 更新时间：2023-12-13

- Expression of serum miR-384 and LIN28B in diabetic retinopathy and their diagnostic value
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 12, Pages: 1059-1062(2023)
- 作者机构：
  
  电子科技大学医学院附属绵阳医院/绵阳市中心医院眼科，四川绵阳 621000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.12E022014
  CLC： R587.2
- Published：25 December 2023，
  
  Received：02 December 2022，
扫描看全文
唐云户,赵媛,代艳.血清miR-384、LIN28B在糖尿病视网膜病变中的表达及其诊断价值分析[J].局解手术学杂志,2023,32(12):1059-1062.

TANG Yun-hu,ZHAO Yuan,DAI Yan.Expression of serum miR-384 and LIN28B in diabetic retinopathy and their diagnostic value[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(12):1059-1062.
唐云户,赵媛,代艳.血清miR-384、LIN28B在糖尿病视网膜病变中的表达及其诊断价值分析[J].局解手术学杂志,2023,32(12):1059-1062. DOI： 10.11659/jjssx.12E022014.

TANG Yun-hu,ZHAO Yuan,DAI Yan.Expression of serum miR-384 and LIN28B in diabetic retinopathy and their diagnostic value[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(12):1059-1062. DOI： 10.11659/jjssx.12E022014.

摘要

目的

探讨糖尿病视网膜病变（DR）患者血清中microRNA-384（miR-384）、LIN28B的表达水平及其诊断价值。

方法

选取2020年1月至2021年11月于本院确诊的100例2型糖尿病患者为研究对象，根据是否发生视网膜病变分为非DR组（40例）、DR组（60例）；另选取同期本院的健康体检者40例作为对照组。采用qRT-PCR法检测血清miR-384、LIN28B相对表达水平，并分析两者与DR患者临床资料的关系。采用

Pearson

法分析DR患者血清中miR-384与LIN28B表达的相关性。采用

Logistic

回归分析DR发生的影响因素。采用受试者工作特征（

ROC

）曲线分析血清miR-384、LIN28B水平对DR患者的诊断价值。

结果

DR组患者血清中miR-384水平明显低于非DR组和对照组（

0.05），且非DR组低于对照组（

0.05）；DR组患者血清中LIN28B水平明显高于非DR组和对照组（

0.05），且非DR组高于对照组（

0.05）。miR-384、LIN28B表达水平与患者糖尿病病程、血糖有关（

0.05）。

Pearson

法分析显示，DR患者血清中miR-384与LIN28B表达呈负相关（

=-0.296，

0.05）。

Logistic

回归分析结果显示，miR-384、LIN28B是DR发生的独立影响因素（

0.05）。

ROC

曲线分析结果显示，miR-384、LIN28B及二者联合检测诊断DR患者的曲线下面积（

AUC

）分别为0.625、0.646、0.682，特异度分别为67.50%、85.00%、97.50%。

结论

DR患者血清中miR-384呈低表达，LIN28B呈高表达，miR-384、LIN28B均是DR发生的影响因素，并且有望成为诊断DR的潜在血清学指标。

Abstract

Objective

To investigate the expression levels of microRNA-384 （miR-384） and LIN28B in serum of patients with diabetic retinopathy （DR） and their diagnostic value.

Methods

A total of 100 patients with type 2 diabetes diagnosed in our hospital from January 2020 to November 2021 were selected as the research subjects. According to the occurrence of retinopathy， the patients were divided into the non-DR group（40 cases） and the DR group（60 cases）. In addition， 40 healthy person who underwent physical examination in our hospital during the same period were regarded as the control group. The relative expression levels of serum miR-384 and LIN28B were detected by qRT-PCR， and the relationship between the two indexes and the general clinical data of DR patients was analyzed.

Pearson

method was applied to analyze the correlation between the expression of miR-384 and LIN28B in serum of DR patients.

Logistic

regression was applied to analyze the influencing factors of DR. The diagnostic value of serum miR-384 and LIN28B levels for DR patients was analyzed by

receiver operating characteristic

（

ROC

） curve.

Results

The level of serum miR-384 in the DR group was obviously lower than that in the non-DR group and the control group（

0.05）， and the non-DR group was lower than the control group （

0.05）. The level of serum LIN28B in the DR group was obviously higher than that in the non-DR group and the control group （

0.05）， and the non-DR group was higher than the control group （

0.05）. The expression levels of miR-384 and LIN28B were related to the course of diabetes and blood glucose （

0.05）.

Pearson's

correlation analysis showed that the expression of miR-384 was negatively correlated with LIN28B in serum of DR patients （

=-0.296，

0.05）.

Logistic

regression analysis showed that miR-384 and LIN28B were the independent influencing factors for DR （

0.05）. The results of

ROC

curve analysis showed that the

area under the curve

（

AUC

） of miR-384， LIN28B and the combination of two in DR patients was 0.625， 0.646， and 0.682， respectively， and the specificity was 67.50%， 85.00%， and 97.50%， respectively.

Conclusion

The serum expression of miR-384 is low and LIN28B is high in DR patients. Both miR-384 and LIN28B are the influencing factors for the occurrence of DR and are expected to be potential serological markers for the diagnosis of DR.

关键词

糖尿病视网膜病变miR-384LIN28B诊断价值

Keywords

diabetic retinopathymicroRNA-384LIN28Bdiagnostic value

references

Simó-Servat O， Hernández C， Simó R. Diabetic retinopathy in the context of patients with diabetes［J］. Ophthalmic Res， 2019， 62（4）：211-217. doi： 10.1159/000499541http://dx.doi.org/10.1159/000499541.

仇秀娟，刘彦铄，刘凤桐. 全视网膜激光光凝对糖尿病视网膜病变患者黄斑区脉络膜厚度的影响及与预后的相关性［J］. 局解手术学杂志，2022，31（3）：216-220. doi：10.11659/jjssx.08E021065http://dx.doi.org/10.11659/jjssx.08E021065.

Lin KY， Hsih WH， Lin YB， et al. Update in the epidemiology， risk factors， screening， and treatment of diabetic retinopathy［J］. J Diabetes Investig，2021，12（8）：1322-1325. doi： 10.1111/jdi.13480http://dx.doi.org/10.1111/jdi.13480.

何琏，李珊，季学磊.老年2型糖尿病病人血红蛋白和糖化血红蛋白水平与视网膜病变风险的相关研究［J］.实用老年医学，2020，34（12）：1243-1245. doi：10.3969/j.issn.1003-9198.2020. 12.008http://dx.doi.org/10.3969/j.issn.1003-9198.2020.12.008.

Han N， Xu H， Yu N， et al. MiR-203a-3p inhibits retinal angiogenesis and alleviates proliferative diabetic retinopathy in oxygen-induced retinopathy （OIR） rat model via targeting VEGFA and HIF-1α［J］. Clin Exp Pharmacol Physiol， 2020， 47（1）：85-94. doi：10.1111/1440-1681.13163http://dx.doi.org/10.1111/1440-1681.13163.

Xia F， Sun JJ， Jiang YQ， et al. MicroRNA-384-3p inhibits retinal neovascularization through targeting hexokinase 2 in mice with diabetic retinopathy［J］. J Cell Physiol， 2018， 234（1）：721-730. doi： 10.1002/jcp.26871http://dx.doi.org/10.1002/jcp.26871.

马楠，苏鹏程.Lin28B在三阴乳腺癌的表达及作用机制分析［J］.临床医药文献电子杂志，2019，6（22）：104.

Zhang W， Sui Y. CircBPTF knockdown ameliorates high glucose-induced inflammatory injuries and oxidative stress by targeting the miR-384/LIN28B axis in human umbilical vein endothelial cells［J］. Mol Cell Biochem， 2020， 471（1-2）：101-111. doi：10.1007/s11010-020-03770-2http://dx.doi.org/10.1007/s11010-020-03770-2.

翁建平.中国2型糖尿病防治指南（2013年版）［J］.中华内分泌代谢杂志，2014，30（10）：893-942. doi：10.3760/cma.j.issn.1000-6699.2014.10.020http://dx.doi.org/10.3760/cma.j.issn.1000-6699.2014.10.020.

Lechner J， O'Leary OE， Stitt AW. The pathology associated with diabetic retinopathy［J］. Vision Res， 2017， 139（1）：7-14. doi：10.1016/j.visres.2017.04.003http://dx.doi.org/10.1016/j.visres.2017.04.003.

Wang W， Lo ACY. Diabetic retinopathy： pathophysiology and treatments［J］. Int J Mol Sci， 2018， 19（6）：1816. doi：10.3390/ijms19061816http://dx.doi.org/10.3390/ijms19061816.

刘琪，王绍飞，丁琳，等.血清miR-211对糖尿病视网膜病变的诊断价值及其影响因素分析［J］.临床检验杂志，2021，39（6）：445-448. doi：10.13602/j.cnki.jcls.2021.06.10http://dx.doi.org/10.13602/j.cnki.jcls.2021.06.10.

蒙秋，黄守国.CircKIF4A靶向调控miR-384表达对卵巢癌SKOV3细胞增殖和凋亡的影响［J］.中国妇产科临床杂志，2021，22（6）：635-638. doi：10.13390/j.issn.1672-1861.2021.06.021http://dx.doi.org/10.13390/j.issn.1672-1861.2021.06.021.

刘皎皎，杨跃青，戴玲，等.microRNA-384靶向HDAC9调控肝癌细胞增殖迁移侵袭和凋亡的机制研究［J］.中国中西医结合消化杂志，2020，28（8）：573-579. doi：10.3969/j.issn.1671-038X. 2020.08.02http://dx.doi.org/10.3969/j.issn.1671-038X.2020.08.02.

Bao Y， Lin C， Ren J， et al. MicroRNA-384-5p regulates ischemia-induced cardioprotection by targeting phosphatidylinositol-4，5-bisphosphate 3-kinase， catalytic subunit delta （PI3K p110δ）［J］. Apoptosis，2013， 18（3）：260-270. doi： 10.1007/s10495-013-0802-1http://dx.doi.org/10.1007/s10495-013-0802-1.

Huang Q， Niu Y， Song L， et al. Does LIN28B gene dysregulation make women more likely to abort？［J］. Reprod Fertil，2021， 2（3）：211-220. doi：10.1530/RAF-21-0033http://dx.doi.org/10.1530/RAF-21-0033.

West RC， McWhorter ES， Ali A， et al. HMGA2 is regulated by LIN28 and BRCA1 in human placental cells［J］. Biol Reprod， 2019， 100（1）：227-238. doi：10.1093/biolre/ioy183http://dx.doi.org/10.1093/biolre/ioy183.

Ma L， Zhao Q， Chen W， et al. Oncogene Lin28B increases chemosensitivity of colon cancer cells in a let-7-independent manner［J］. Oncol Lett， 2018， 15（5）：6975-6981. doi： 10.3892/ol.2018. 8250http://dx.doi.org/10.3892/ol.2018.8250.

Chen D， Cox J， Annam J， et al. LIN28B promotes neuroblastoma metastasis and regulates PDZ binding kinase［J］. Neoplasia， 2020， 22（6）：231-241. doi：10.1016/j.neo.2020.04.001http://dx.doi.org/10.1016/j.neo.2020.04.001.

Brennan E， Wang B， McClelland A， et al. Protective effect of let-7 miRNA family in regulating inflammation in diabetes-associated atherosclerosis［J］. Diabetes， 2017， 66（8）：2266-2277. doi：10.2337/db16-1405http://dx.doi.org/10.2337/db16-1405.

Fu X， Ou B. miR-152/LIN28B axis modulates high-glucose-induced angiogenesis in human retinal endothelial cells via VEGF signaling［J］. J Cell Biochem， 2020， 121（2）：954-962. doi：10.1002/jcb.28978http://dx.doi.org/10.1002/jcb.28978.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

糖尿病视网膜病变；黄斑区脉络膜厚度；全视网膜激光光凝；最佳矫正视力；影响因素

Related Author

孙成

孟晓红

张敏芳

冉黎

QIU Xiu-juan

LIU Yan-shuo

LIU Feng-tong

Related Institution

第三军医大学西南医院眼科

Department of Ophthalmology,Beijing Friendship Hospital Pinggu Campus,Capital Medical University

⁰