Correlation analysis of miR-4500, NGF expressions with preoperative magnetic resonance signs in breast cancer

YANG Fan; CHEN Jing; ZHENG Bin; LI Jiao-jiao; ZHANG Li; BIAN Gui-feng; BIAN Ya-jing; LANG Xiao-yan

doi:10.11659/jjssx.12E022002

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Correlation analysis of miR-4500, NGF expressions with preoperative magnetic resonance signs in breast cancer

Clinical Research | 更新时间：2024-01-22

- Correlation analysis of miR-4500, NGF expressions with preoperative magnetic resonance signs in breast cancer
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 33, Issue 1, Pages: 47-50(2024)
- 作者机构：
  
  河北北方学院附属第一医院医学影像部，河北张家口 075000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.12E022002
  CLC： R445.2;R737.9
- Published：25 January 2024，
  
  Received：01 December 2022，
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杨帆,陈静,张斌等.乳腺癌miR-4500、NGF表达与术前磁共振征象的相关性研究[J].局解手术学杂志,2024,33(01):47-50.

YANG Fan,CHEN Jing,ZHENG Bin,et al.Correlation analysis of miR-4500, NGF expressions with preoperative magnetic resonance signs in breast cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):47-50.
杨帆,陈静,张斌等.乳腺癌miR-4500、NGF表达与术前磁共振征象的相关性研究[J].局解手术学杂志,2024,33(01):47-50. DOI： 10.11659/jjssx.12E022002.

YANG Fan,CHEN Jing,ZHENG Bin,et al.Correlation analysis of miR-4500, NGF expressions with preoperative magnetic resonance signs in breast cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):47-50. DOI： 10.11659/jjssx.12E022002.

摘要

目的

分析乳腺癌患者中miR-4500、神经生长因子（NGF）表达与术前磁共振（MR）征象的相关性。

方法

选取我院收治的乳腺癌患者105例，通过qRT-PCR法检测患者乳腺癌组织及癌旁组织中miR-4500、NGF mRNA表达水平，免疫组化法测定乳腺癌组织及癌旁组织中NGF的阳性表达率。分析乳腺癌组织中miR-4500、NGF表达与患者临床病理特征及MR征象的相关性。生物信息网站预测miR-4500与NGF的靶向关系，并分析二者表达的相关性。

结果

与癌旁组织相比，乳腺癌组织中miR-4500表达水平降低（

0.05），NGF mRNA水平、NGF阳性表达率升高（

0.05）。年龄、病理类型、肿瘤分型、实质背景、表观弥散系数、强化曲线在不同miR-4500表达及NGF表达间差异无统计学意义（

0.05），组织学分级、淋巴结转移、肿瘤直径、肿瘤形态、环形强化、瘤周水肿与miR-4500表达及NGF表达有关（

0.05）。生物信息网站预测显示，miR-4500与NGF存在结合位点，乳腺癌组织中miR-4500表达与NGF mRNA表达呈负相关（

=-0.576，

0.05）。

结论

乳腺癌组织miR-4500低表达，NGF高表达，二者与患者术前MR征象具有相关性，为MR征象提供了分子学依据。

Abstract

Objective

To analyze the correlations of the expressions of miR-4500 and nerve growth factor （NGF） in breast cancer patients with preoperative magnetic resonance （MR） signs.

Methods

A total of 105 patients with breast cancer admitted to our hospital were selected， the mRNA expression levels of miR-4500 and NGF in breast cancer tissues and adjacent tissues of patients were detected by qRT-PCR， and the positive expression rates of NGF in breast cancer tissues and adjacent tissues were determined by immunohistochemistry method. The correlations of the expressions of miR-4500 and NGF in breast cancer tissues with clinicopathological features and MR signs of patients were analyzed. The targeting relationship between miR-4500 and NGF was predicted by the bioinformatics website， and the correlation between the expressions of the two was analyzed.

Results

Compared with the adjacent tissues， the expression level of miR-4500 in breast cancer tissue decreased （

0.05）， while the level of NGF mRNA and the positive expression rate of NGF increased （

0.05）.There was no significant difference in age， pathological type， tumor classification， parenchymal background， apparent diffusion coefficient or enhancement curve among different expressions of miR-4500 and NGF （

0.05）. The histological grade， lymph node metastasis， tumor diameter， tumor morphology， ring-like enhancement， and peritu-moral brain edema were related to the expressions of miR-4500 and NGF（

0.05）. The bioinformatics website prediction showed that miR-4500 and NGF had binding sites， and the expressions of miR-4500 and NGF mRNA in breast cancer tissues were negatively correlated （

=-0.576，

0.05）.

Conclusion

The expression of miR-4500 in breast cancer tissue is low， and the expression of NGF is high， which are correlated with the preoperative MR signs in patients， providing a molecular basis for MR signs.

关键词

乳腺癌miR-4500神经生长因子MR征象

Keywords

breast cancermiR-4500nerve growth factorMR signs

references

da Silva BAO，Dias IS，Sarto LE，et al.Cytotoxicity induced by newly synthesized palladium （Ⅱ） complexes lead to the death of MCF-7 and MDA-MB-435 cancer cell lines［J］.Adv Pharm Bull，2023，13（1）：160-169.doi：10.34172/apb.2023.017http://dx.doi.org/10.34172/apb.2023.017.

Min J，Kim JY，Yeon S，et al.Change in shoulder function in the early recovery phase after breast cancer surgery：a prospective observational study［J］.J Clin Med，2021，10（15）：3416.doi：10.3390/jcm10153416http://dx.doi.org/10.3390/jcm10153416.

Khalki LE，Maire V，Dubois T，et al.Berberine impairs the survival of triple negative breast cancer cells：cellular and molecular analyses［J］.Molecules，2020，25（3）：506.doi：10.3390/molecules25030506http://dx.doi.org/10.3390/molecules25030506.

呙月，刘诗月，黄玉琴，等.miR-4500通过转录后下调LIN28B表达抑制乳腺癌细胞的迁移和增殖［J］.山西医科大学学报，2020，51（2）：119-123.doi：10.13753/j.issn.1007-6611.2020.02.003http://dx.doi.org/10.13753/j.issn.1007-6611.2020.02.003.

Li S，Mai H，Zhu Y，et al.MicroRNA-4500 inhibits migration，invasion and angiogenesis of breast cancer cells via RRM2-dependent MAPK signaling pathway［J］.Mol Ther Nucleic Acids，2020，21：278-289.doi：10.1016/j.omtn.2020.04.018http://dx.doi.org/10.1016/j.omtn.2020.04.018.

Jafari M，Delaviz H，Torabi S，et al.The effect of muscle graft with nerve growth factor and laminin on sciatic nerve repair in rats［J］.Basic Clin Neurosci，2019，10（4）：333-344.doi：10.32598/bcn.9.10.145http://dx.doi.org/10.32598/bcn.9.10.145.

Bruno F，Arcuri D，Vozzo F，et al.Expression and signaling pathways of nerve growth factor （NGF） and pro-NGF in breast cancer：a systematic review［J］.Curr Oncol，2022，29（11）：8103-8120.doi：10.3390/curroncol29110640http://dx.doi.org/10.3390/curroncol29110640.

朱景航，高小玲.MR征象与乳腺癌VEGF表达的相关性分析［J］.影像诊断与介入放射学，2021，30（4）：261-265.doi：10.3969/j.issn.1005-8001.2021.04.004http://dx.doi.org/10.3969/j.issn.1005-8001.2021.04.004.

Wilkinson L，Gathani T.Understanding breast cancer as a global health concern［J］.Br J Radiol，2022，95（1130）：20211033.doi：10.1259/bjr.20211033http://dx.doi.org/10.1259/bjr.20211033.

Ding W，Fan Z，Xu Y，et al.Magnetic resonance imaging in screening women at high risk of breast cancer：a meta-analysis［J］.Medicine （Baltimore），2023，102（10）：e33146.doi：10.1097/MD.0000000000033146http://dx.doi.org/10.1097/MD.0000000000033146.

Bruno F，Abondio P，Montesanto A，et al.The nerve growth factor receptor （NGFR/p75NTR）：a major player in Alzheimer's disease［J］.Int J Mol Sci，2023，24（4）：3200.doi：10.3390/ijms24043200http://dx.doi.org/10.3390/ijms24043200.

Vera DB，Fredes AN，Garrido MP，et al.Role of mitochondria in interplay between NGF/TRKA，miR-145 and possible therapeutic strategies for epithelial ovarian cancer［J］.Life，2021，12（1）：8.doi：10.3390/life12010008http://dx.doi.org/10.3390/life12010008.

Mazaki A，Yamauchi K，Orita S，et al.Nerve growth factor in breast cancer cells promotes axonal growth and expression of calcitonin gene-related peptide in a rat model of spinal metastasis［J］.Anticancer Res，2022，42（1）：581-587.doi：10.21873/anticanres.15515http://dx.doi.org/10.21873/anticanres.15515.

Li ZY，Zhang ZZ，Bi H，et al.MicroRNA4500 suppresses tumor progression in nonsmall cell lung cancer by regulating STAT3［J］.Mol Med Rep，2019，20（6）：4973-4983.doi：10.3892/mmr.2019.10737http://dx.doi.org/10.3892/mmr.2019.10737.

Fu D，Ren Y，Wang C，et al.LINC01287 facilitates proliferation，migration，invasion and EMT of colon cancer cells via miR-4500/MAP3K13 pathway［J］.BMC Cancer，2021，21（1）：782.doi：10.1186/s12885-021-08528-7http://dx.doi.org/10.1186/s12885-021-08528-7.

Peng W，Dong N，Wu S，et al.miR‐4500 suppresses cell proliferation and migration in bladder cancer via inhibition of STAT3/CCR7 pathway［J］.J Cell Biochem，2019，121（8/9）：3913-3922.doi：10.1002/jcb.29558http://dx.doi.org/10.1002/jcb.29558.

岳叶洼团，熊春来.磁共振T2WI序列瘤周水肿征象用于润性乳腺癌早期复发诊断的价值分析［J］.影像研究与医学应用，2021，5（21）：44-46.doi：10.3969/j.issn.2096-3807.2021.21.021http://dx.doi.org/10.3969/j.issn.2096-3807.2021.21.021.

Lee HJ，Lee JE，Jeong WG，et al.HER2-positive breast cancer：association of MRI and clinic opathologic features with tumor-infiltrating lymphocytes［J］.AJR Am J Roentgenol，2022，218（2）：258-269.doi：10.2214/AJR.21.26400http://dx.doi.org/10.2214/AJR.21.26400.

黎冠峰.MR动态增强对不同组织类型乳腺癌的诊断及预后评价［J］.现代医用影像学，2019，28（2）：240-243.

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