Expression and biological role of DCST1-AS1 in non-small cell lung cancer

GUO Xiao-dan; MA Zhen-yu; WANG Chen-jing; HAN Tian-yun; ZHANG Fei-yan

doi:10.11659/jjssx.11E022106

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Expression and biological role of DCST1-AS1 in non-small cell lung cancer

Basic Research | 更新时间：2023-12-13

- Expression and biological role of DCST1-AS1 in non-small cell lung cancer
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 12, Pages: 1035-1039(2023)
- 作者机构：
  
  1.保定市第二医院病理科，河北保定 071000
  2.保定市第二医院药剂科，河北保定 071000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.11E022106
  CLC： R734.2
- Published：25 December 2023，
  
  Received：21 November 2022，
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郭晓丹,马振禹,王晨静等.DCST1-AS1在非小细胞肺癌中的表达及其生物学作用[J].局解手术学杂志,2023,32(12):1035-1039.

GUO Xiao-dan,MA Zhen-yu,WANG Chen-jing,et al.Expression and biological role of DCST1-AS1 in non-small cell lung cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(12):1035-1039.
郭晓丹,马振禹,王晨静等.DCST1-AS1在非小细胞肺癌中的表达及其生物学作用[J].局解手术学杂志,2023,32(12):1035-1039. DOI： 10.11659/jjssx.11E022106.

GUO Xiao-dan,MA Zhen-yu,WANG Chen-jing,et al.Expression and biological role of DCST1-AS1 in non-small cell lung cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(12):1035-1039. DOI： 10.11659/jjssx.11E022106.

摘要

目的

检测DCST1-AS1在非小细胞肺癌中的表达，并探讨其对癌细胞恶性生物学行为的影响。

方法

收集65例非小细胞肺癌患者的癌组织及其癌旁组织，体外培养正常人支气管上皮细胞16HBE和非小细胞肺癌细胞系（A549、H1299、H1650和HCC827）。RT-qPCR法检测组织和细胞中DCST1-AS1、miR-29b表达水平，并分析DCST1-AS1表达与非小细胞肺癌患者临床特征的相关性。将A549细胞分为对照组、si-NC组、si-DCST1-AS1组、si-DCST1-AS1+anti-NC组和si-DCST1-AS1+anti-miR-29b组。采用CCK-8法、克隆形成实验检测细胞增殖；Transwell检测细胞侵袭及迁移；Western blot检测E-cadherin、Vimentin、N-cadherin表达。

结果

非小细胞肺癌组织和细胞中DCST1-AS1的表达均升高（

0.05），miR-29b的表达均降低（

0.05）；DCST1-AS1的表达与非小细胞肺癌患者TNM分期、组织分化程度、淋巴结转移及病理类型有关（

0.05）。与对照组或si-NC组比较，si-DCST1-AS1组A549细胞中DCST1-AS1表达、OD值、克隆形成数、迁移细胞数、侵袭细胞数及Vimentin、N-cadherin表达降低（

0.05），miR-29b、E-cadherin表达升高（

0.05）。敲低miR-29b可明显减弱下调DCST1-AS1表达对A549细胞恶性生物学行为的影响。

结论

在非小细胞肺癌中DCST1-AS1呈高表达，敲低DCST1-AS1可能通过上调miR-29b表达抑制非小细胞肺癌细胞的恶性生物学行为。

Abstract

Objective

To detect the expression of DCST1-AS1 in non-small cell lung cancer and explore its effect on malignant biological behavior of cancer cells.

Methods

The tissues and corresponding adjacent tissues from 65 patients with non-small cell lung cancer were collected， and the normal human bronchial epithelial cells 16HBE and the non-small cell lung cancer cell lines （A549， H1299， H1650 and HCC827） were cultured in vitro. The expression levels of DCST1-AS1 and miR-29b in tissues and cells were detected by RT-qPCR assay， and the correlation between the DCST1-AS1 expression and the clinical characteristics of patients with non-small cell lung cancer were analyzed. A549 cells were divided into the control group， the si-NC group， the si-DCST1-AS1 group， the si-DCST1-AS1+anti-NC group and the si-DCST1-AS1+anti-miR-29b group. The cell proliferation was detected by CCK-8 assay and clone formation assay，the invasion and migration of cells were detected by Transwell； the expression of E-cadherin， Vimentin and N-cadherin was detected by Western blot.

Results

The expression of DCST1-AS1 increased and the expression of miR-29b decreased in non-small cell lung cancer tissues and cells （

0.05）. The expression of DCST1-AS1 was correlated with TNM stage， differentiated degree of tissue， lymph node metastasis and pathological types in non-small cell lung cancer patients（

0.05）. Compared with the control group or the si-NC group， the expression of DCST1-AS1， OD value， number of colony-forming cells， migration cells and invasion cells and the expression of Vimentin and N-cadherin in A549 cells of the si-DCST1-AS1 group decreased（

0.05）， while the expression of miR-29b and E-cadherin increased （

0.05）. Knocking down miR-29b could significantly reduce the effect of down-regulation of DCST1-AS1 expression on the malignant biological behavior of A549 cells.

Conclusion

DCST1-AS1 is highly expressed in non-small cell lung cancer， knocking down DCST1-AS1 may inhibit the malignant biological behavior of non-small cell lung cancer cells by up-regulating the expression of miR-29b.

关键词

非小细胞肺癌DCST1-AS1miR-29b细胞增殖迁移侵袭

Keywords

non-small cell lung cancerDCST1-AS1miR-29bcell proliferationmigrationinvasion

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