Neuroprotective effect of Zhenbao pill mediated NF-κB/p65 pathway on rats with spinal cord injury

WANG Xing; LI Si-qin; ZHANG Bao-xin

doi:10.11659/jjssx.10E023135

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Neuroprotective effect of Zhenbao pill mediated NF-κB/p65 pathway on rats with spinal cord injury

Basic Research | 更新时间：2024-10-22

- Neuroprotective effect of Zhenbao pill mediated NF-κB/p65 pathway on rats with spinal cord injury
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 33, Issue 10, Pages: 887-891(2024)
- 作者机构：
  
  1.巴彦淖尔市医院脊柱科，内蒙古巴彦淖尔 015000
  2.巴彦淖尔市医院神经外科，内蒙古巴彦淖尔 015000
  3.内蒙古医科大学第二附属医院创伤骨科，内蒙古呼和浩特 010050
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.10E023135
  CLC： R285.5
- Published：25 October 2024，
  
  Received：30 October 2023，
- 稿件说明：
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王兴,李斯琴,张葆鑫.珍宝丸介导NF-κB/p65通路对脊髓损伤大鼠的神经保护作用[J].局解手术学杂志,2024,33(10):887-891.

WANG Xing,LI Si-qin,ZHANG Bao-xin.Neuroprotective effect of Zhenbao pill mediated NF-κB/p65 pathway on rats with spinal cord injury[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(10):887-891.
王兴,李斯琴,张葆鑫.珍宝丸介导NF-κB/p65通路对脊髓损伤大鼠的神经保护作用[J].局解手术学杂志,2024,33(10):887-891. DOI： 10.11659/jjssx.10E023135.

WANG Xing,LI Si-qin,ZHANG Bao-xin.Neuroprotective effect of Zhenbao pill mediated NF-κB/p65 pathway on rats with spinal cord injury[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(10):887-891. DOI： 10.11659/jjssx.10E023135.

摘要

目的

探讨珍宝丸对脊髓损伤大鼠的神经保护作用及相关机制。

方法

60只SD大鼠随机均分为对照组、模型组和珍宝丸组，对照组大鼠仅暴露脊髓，模型组和珍宝丸组大鼠均构建脊髓损伤模型。造模成功后，珍宝丸组大鼠给予口服0.6 g/kg珍宝丸干预治疗，间隔24 h给药1次，持续给药28 d，对照组和模型组大鼠则给予相同剂量的生理盐水灌胃。采用巴索·比蒂·布雷斯纳汉（BBB）评分法评估干预治疗前和治疗后4周各组大鼠运动行为学变化；实验结束取大鼠脊髓组织标本，通过HE染色和TUNEL染色法观察评估大鼠脊髓组织病理学变化及神经细胞凋亡情况；通过qRT-PCR法检测炎症因子TNF-α、IL-6和IL-10的表达情况；采用Western blot法检测核因子κB（NF-κB）/p65通路相关蛋白的表达情况。

结果

干预治疗后4周，模型组大鼠BBB评分低于对照组，珍宝丸组大鼠BBB评分高于模型组，差异均有统计学意义(

0.05)。对照组大鼠脊髓组织结构、形态正常；模型组大鼠脊髓组织结构紊乱，有明显出血、坏死、水肿，大量炎性细胞浸润；珍宝丸组大鼠脊髓组织病变程度较模型组明显减轻。珍宝丸组脊髓组织细胞凋亡数较模型组减少，差异有统计学意义（

0.05）。与对照组相比，模型组脊髓组织中TNF-α和IL-6表达升高，IL-10表达下降，差异均有统计学意义（

0.05）；与模型组相比，珍宝丸组脊髓组织中TNF-α和IL-6表达显著降低，IL-10表达显著升高，差异均有统计学意义（

0.05）。模型组大鼠脊髓组织中NF-κB/p65和p-NF-κB/p65蛋白表达均较对照组上调，差异有统计学意义（

0.05）；珍宝丸组大鼠脊髓组织中NF-κB/p65和p-NF-κB/p65蛋白表达均较模型组下调，差异有统计学意义（

0.05）。

结论

珍宝丸治疗能够有效改善脊髓损伤大鼠行为学症状，减轻其病理变化，具有神经保护作用，其作用机制可能与阻断NF-κB/p65通路、抑制炎症因子表达有关。

Abstract

Objective

To explore the neuroprotective effect and related mechanism of Zhenbao pill on rats with spinal cord injury.

Methods

A total of 60 SD rats were randomly divided into the control group， the model group and the Zhenbao pill group. Rats in the control group only exposed the spinal cord， and rats in the model group and the Zhenbao pill group established spinal cord injury models. After the model was successfully established， rats in the Zhenbao Pill group was given 0.6 g/kg Zhenbao pill orally once every 24 hours for 28 days， and rats in the control group and the model group were given the same dose of normal saline by gavage. Basso，Beattie，Bresnahan （BBB） score was used to evaluate the changes in motor behavior of rats in each group before and 4 weeks after intervention treatment. At the end of the experiment， the spinal cord tissue samples were collected， and the histopathological changes and apoptosis of nerve cells were observed and evaluated by HE staining and TUNEL staining. The expression of inflammatory factors TNF-α， IL-6 and IL-10 was detected by qRT-PCR. The expression of nuclear factor κB （NF-κB）/p65 pathway related proteins was detected by Western blot.

Results

After 4 weeks of intervention treatment，the BBB score of rats in the model group was lower than that in the control group， and the BBB score of rats in the Zhenbao pill group was higher than that in the model group， and the differences were statistically significant （

0.05）. The structure and morphology of spinal cord tissue of rats in the control group were normal； the spinal cord tissue structure of rats in the model group was disordered，with obvious bleeding， necrosis and edema，and a large number of inflammatory cells infiltrated；the degree of lesion of spinal cord tissue of rats in the Zhenbao pill gr

oup was significantly reduced than that in the model group.The number of apoptosis of spinal cord tissue in the Zhenbao pill group was decreased compared with that in the model group，and the difference was statistically significant （

0.05）. Compared with the control group， the expressions of TNF-α and IL-6 in spinal cord tissue in the model group were increased， while the expression of IL-10 was decreased，with statistically significant differences （

0.05）.Compared with the model group，the expressions of TNF-α and IL-6 in spinal cord tissue in the Zhenbao pill group were significantly decreased， while the expression of IL-10 was increased， with statistically significant differences （

0.05）. The protein expressions of NF-κB/p65 and p-NF-κB/p65 in spinal cord tissue of rats in the model group was up-regulated compared with those in the control group， and the differences were statistically significant （

0.05）； the protein expressions of NF-κB/p65 and p-NF-κB/p65 in spinal cord tissue of rats in the Zhenbao pill group were down-regulated compared with those in the model group， and the differences were statistically significant （

0.05）.

Conclusion

Zhenbao pill can effectively improve the behavioral symptoms of rats with spinal cord injury， alleviate its pathological changes， which has neuroprotective effects. Its mechanism may be related to blocking the NF-κB/p65 pathway and inhibiting the expression of inflammatory factors.

关键词

珍宝丸脊髓损伤神经损伤NF-κB/p65通路

Keywords

Zhenbao pillspinal cord injurynerve injuryNF-κB/p65 pathway

references

Badhiwala JH，Wilson JR，Fehlings MG.Global burden of traumatic brain and spinal cord injury［J］.Lancet Neurol，2019，18（1）：24-25.doi：10.1016/S1474-4422（18）30444-7http://dx.doi.org/10.1016/S1474-4422（18）30444-7.

Karsy M，Hawryluk G.Modern medical management of spinal cord injury［J］.Curr Neurol Neurosci Rep，2019，19（9）：65.doi：10.1007/s11910-019-0984-1http://dx.doi.org/10.1007/s11910-019-0984-1.

Hutson TH，Di Giovanni S.The translational landscape in spinal cord injury：focus on neuroplasticity and regeneration［J］.Nat Rev Neurol，2019，15（12）：732-745.doi：10.1038/s41582-019-0280-3http://dx.doi.org/10.1038/s41582-019-0280-3.

Zhang Q，Michael JL，Baltimore D.30 years of NF-κB：a blossoming of relevance to human pathobiology［J］.Cell，2017，168（1）：37-57.doi：10.1016/j.cell.2016.12.012http://dx.doi.org/10.1016/j.cell.2016.12.012.

Anjum A，Yazid MD，Fauzi Daud M，et al.Spinal cord injury：pathophysiology，multimolecular interactions，and underlying recovery mechanisms［J］.Int J Mol Sci，2020，21（20）：7533.doi：10.3390/ijms21207533http://dx.doi.org/10.3390/ijms21207533.

宝莉莉，宋福顺，都格尔，等.蒙药额尔敦-乌日勒的研究进展［J］.中国民族民间医药，2019，28（3）：53-56.doi：10.3969/j.issn.1007-8517.2019.3.zgmzmjyyzz201903014http://dx.doi.org/10.3969/j.issn.1007-8517.2019.3.zgmzmjyyzz201903014.

朱晓宇，王红月，李怀平，等.如意珍宝丸对斑马鱼神经损伤的保护及促再生作用研究［J］.药物评价研究，2017，40（3）：307-313.doi：10.7501/j.issn.1674-6376.2017.03.004http://dx.doi.org/10.7501/j.issn.1674-6376.2017.03.004.

金光明，李军，冀小伟，等.蒙药珍宝丸治疗脑梗死后遗症的疗效观察［J］.中国民族医药杂志，2021，27（7）：3.doi：10.3969/j.issn.1006-6810.2021.07.003http://dx.doi.org/10.3969/j.issn.1006-6810.2021.07.003.

飞翔，哈斯巴拉，道日娜，等.蒙药额日敦·乌日勒对急性脊髓损伤大鼠神经元凋亡的影响及机制研究［J］.实用临床医药杂志，2023，27（22）：37-43.doi：10.7619/jcmp.20232286http://dx.doi.org/10.7619/jcmp.20232286.

陶名，贺永雄.脊髓损伤药物治疗研究进展［J］.内蒙古医学杂志，2022，54（11）：1340-1342.doi：10.16096/J.cnki.nmgyxzz. 2022.54.11.016http://dx.doi.org/10.16096/J.cnki.nmgyxzz.2022.54.11.016.

Barros Filho TE，Molina AE.Analysis of the sensitivity and reproducibility of the Basso，Beattie，Bresnahan （BBB） scale in Wistar rats［J］.Clinics （Sao Paulo），2008，63（1）：103-108.doi：10.1590/s1807-59322008 000100018http://dx.doi.org/10.1590/s1807-59322008000100018.

Shank CD，Walters BC，Hadley MN.Current topics in the management of acute traumatic spinal cord injury［J］.Neurocrit Care，2019，30（2）：261-271.doi：10.1007/s12028-018-0537-5http://dx.doi.org/10.1007/s12028-018-0537-5.

Zheng Y，Mao YR，Yuan TF，et al.Multimodal treatment for spinal cord injury：a sword of neuroregeneration upon neuromodulation［J］.Neural Regen Res，2020，15（8）：1437-1450.doi：10.4103/1673-5374. 274332http://dx.doi.org/10.4103/1673-5374.274332.

姬智，郭建光，张建宇.珍宝丸对脊髓损伤大鼠NGF的调节作用［J］.内蒙古医学杂志，2023，55（3）：286-287.doi：10.16096/J.cnki.nmgyxzz.2023.55.03.007http://dx.doi.org/10.16096/J.cnki.nmgyxzz.2023.55.03.007.

黄敏聪，杨正标，徐志伟，等.如意珍宝丸对慢性神经源性疼痛大鼠炎症因子水平的影响［J］.中国现代应用药学，2019，36（22）：2790-2793.doi：10.13748/j.cnki.issn1007-7693.2019.22.008http://dx.doi.org/10.13748/j.cnki.issn1007-7693.2019.22.008.

Zhao XC，Zhao XP，Wang ZG.Synergistic neuroprotective effects of hyperbaric oxygen and N-acetylcysteine against traumatic spinal cord injury in rat［J］.J Chem Neuroanat，2021，118：102037.doi：10.1016/j.jchemneu.2021.102037http://dx.doi.org/10.1016/j.jchemneu.2021.102037.

Zarate MA，Nguyen LM，Dios RK，et al.Maturation of the acute hepatic TLR4/NF-κB mediated innate immune response is p65 dependent in mice［J］.Front Immunol，2020，11：1892.doi：10.3389/fimmu.2020. 01892http://dx.doi.org/10.3389/fimmu.2020.01892.

Zhang T，Li K，Zhang ZL，et al.LncRNA Airsci increases the inflammatory response after spinal cord injury in rats through the nuclear factor kappa B signaling pathway［J］.Neural Regen Res，2021，16（4）：772-777.doi：10.4103/1673-5374.295335http://dx.doi.org/10.4103/1673-5374.295335.

Han FX，Zhang R，Yang XX，et al.Neuroprotective effect of aldose reductase knockout in a mouse model of spinal cord injury involves NF-κB pathway［J］.Exp Brain Res，2022，240（3）：853-859.doi：10.1007/s00221-021-06223-4http://dx.doi.org/10.1007/s00221-021-06223-4.

Tang R，Botchway BOA，Meng Y，et al.The inhibition of inflammatory signaling pathway by secretory leukocyte protease inhibitor can improve spinal cord injury［J］.Cell Mol Neurobiol，2020，40（7）：1067-1073.doi：10.1007/s10571-020-00799-1http://dx.doi.org/10.1007/s10571-020-00799-1.

Guo K，Chang Y，Jin Y，et al.circ-Ncam2 （mmu_circ_0006413） participates in LPS-induced microglia activation and neuronal apoptosis via the TLR4/NF-κB pathway［J］.J Mol Neurosci，2022，72（8）：1738-1748.doi：10.1007/s12031-022-02018-6http://dx.doi.org/10.1007/s12031-022-02018-6.

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