Effects of core decompression combined with collagen-based bone repair material and VEGF on bone mineraldensity and biomechanics in rabbits with osteonecrosis of femoral head

LYU Ya-jun; LI Yi-jiong; ZHAO Zhen-shuan; ZHANG Yu-chen; WANG Wei

doi:10.11659/jjssx.10E022102

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Effects of core decompression combined with collagen-based bone repair material and VEGF on bone mineraldensity and biomechanics in rabbits with osteonecrosis of femoral head

Basic Research | 更新时间：2023-07-17

- Effects of core decompression combined with collagen-based bone repair material and VEGF on bone mineraldensity and biomechanics in rabbits with osteonecrosis of femoral head
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 7, Pages: 592-596(2023)
- 作者机构：
  
  河北医科大学第一医院骨二科，河北石家庄 050031
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.10E022102
  CLC： R681.8
- Published：25 July 2023，
  
  Received：19 October 2022，
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吕亚军,李宜炯,赵振拴等.髓芯减压联合胶原基骨修复材料、VEGF对股骨头坏死家兔骨密度及生物力学的影响[J].局解手术学杂志,2023,32(07):592-596.

LYU Ya-jun,LI Yi-jiong,ZHAO Zhen-shuan,et al.Effects of core decompression combined with collagen-based bone repair material and VEGF on bone mineraldensity and biomechanics in rabbits with osteonecrosis of femoral head[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(07):592-596.
吕亚军,李宜炯,赵振拴等.髓芯减压联合胶原基骨修复材料、VEGF对股骨头坏死家兔骨密度及生物力学的影响[J].局解手术学杂志,2023,32(07):592-596. DOI： 10.11659/jjssx.10E022102.

LYU Ya-jun,LI Yi-jiong,ZHAO Zhen-shuan,et al.Effects of core decompression combined with collagen-based bone repair material and VEGF on bone mineraldensity and biomechanics in rabbits with osteonecrosis of femoral head[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(07):592-596. DOI： 10.11659/jjssx.10E022102.

摘要

目的

探讨髓芯减压联合胶原基骨修复材料、血管内皮生长因子（VEGF）对股骨头坏死（ONFH）家兔模型的股骨头骨密度及生物力学的影响。

方法

30只家兔股骨头内注射无水乙醇建立ONFH模型，随机分为健康对照组（A组）、模型组（B组）、髓芯减压联合自体松质骨组（C组）、髓芯减压联合胶原基骨修复材料组（D组）及髓芯减压联合胶原基骨修复材料和VEGF组（E组），每组6只。治疗8周后，分别检测各组家兔股骨头的骨密度和生物力学参数（最大应力、弹性模量、刚度、最大载荷和能量吸收），HE染色观察股骨头形态。qRT-PCR检测各组家兔的股骨头组织中骨保护素（OPG）、血管内皮生长因子164（VEGF164）、血管内皮生长因子受体2（VEGFR2）、核因子κB受体活化因子配体（RANKL）和核因子κB受体活化因子（RANK）的mRNA水平。

结果

B组的骨密度较A组显著降低（

0.05），E组的骨密度显著高于B组、C组和D组（

0.05）。与A组相比，B组的空骨陷窝比率显著增高（

0.05）；与B组相比，C组、D组和E组的空骨陷窝比率均降低，以E组最低（

0.05）。与A组相比，B组的股骨头生物力学参数显著降低（

0.05）；与B组相比，C组、D组和E组的股骨头生物力学参数均升高，以E组最高（

0.05）。与B组、C组和D组相比，E组股骨头组织中OPG、VEGF164和VEGFR2的mRNA水平均升高（

0.05），RANKL和RANK的mRNA水平均降低（

0.05）。

结论

髓芯减压联合胶原基骨修复材料、VEGF可有效提高ONFH家兔股骨头的骨密度和强度，促进损伤区域的骨形成和血管生成，促进坏死性股骨头的修复。

Abstract

Objective

To investigate the effects of core decompression combined with collagen-based bone repair material and vascular endothelial growth factor （VEGF） on bone mineral density and biomechanics of the femoral head in rabbit model of osteonecrosis of femoral head （ONFH）.

Methods

The ONFH model was established by injecting absolute ethanol into the femoral head of 30 rabbits. Then the rabbits were randomly divided into the healthy control group （group A）， the model group （group B）， the core decompression combined with autologous cancellous bone group （group C），the core decompression combined with collagen-based bone repair material group （group D） and the core decompression combined with collagen-based bone repair material and VEGF group （group E）， with 6 rabbits in each group. After 8 weeks of treatment， the bone mineral density and biomechanical parameters （maximum stress， elastic modulus， stiffness， maximum load and energy absorption） of the femoral head of rabbits in each group were detected respectively， and the morphology of the femoral head was observed by HE staining. The mRNA levels of osteoprotegerin （OPG）， vascular endothelial growth factor 164 （VEGF164）， vascular endothelial growth factor receptor 2 （VEGFR2）， receptor activator of nuclear factor-κB ligand （RANKL） and receptor activator of nuclear factor-κB （RANK） in the femoral head tissue of rabbits in each group were detected by qRT-PCR.

Results

The bone mineral density of group B was significantly lower than that of group A （

0.05）， and the bone mineral density of group E was significantly higher than those of group B， group C and group D （

0.05）. Compared with group A， the ratio of empty bone lacunae in group B was significantly higher （

0.05）. Compared with group B， the ratios of empty bone lacunae in group C， group D and group E were decreased， and group E was the lowest （

0.05）. Compared with group A， the biomechanical parameters of the femoral head in group B were significantly decreased （

0.05）. Compared with group B， the biomechanical parameters of the femoral head in group C， group D and group E were increased， and group E was the highest （

0.05）. Compared with group B， group C and group D， the mRNA levels of OPG， VEGF164 and VEGFR2 in the femoral head tissue of group E were increased（

0.05）， while the mRNA levels of RANKL and RANK were decreased （

0.05）.

Conclusion

Core decompression combined with collagen-based bone repair material and VEGF can effectively improve the bone mineral density and strength of the femoral head of ONFH rabbits， promote bone formation and angiogenesis in the injured area， and promote the repair of necrotic femoral head.

关键词

股骨头坏死髓芯减压胶原基骨修复材料血管内皮生长因子骨密度生物力学

Keywords

osteonecrosis of femoral headcore decompressioncollagen-based bone repair materialvascular endothelial growth factorbone mineral densitybiomechanics

references

Cohen-Rosenblum A，Cui Q.Osteonecrosis of the femoral head［J］.Orthop Clin North Am，2019，50（2）：139-149.doi：10.1016/j.ocl.2018.10.001http://dx.doi.org/10.1016/j.ocl.2018.10.001.

Jing X，Du T，Yang X，et al.Desferoxamine protects against glucocorticoid-induced osteonecrosis of the femoral head via activating HIF-1α expression［J］.J Cell Physiol，2020，235（12）：9864-9875.doi：10.1002/jcp.29799http://dx.doi.org/10.1002/jcp.29799.

Yin BH，Chen HC，Zhang W，et al.Effects of hypoxia environment on osteonecrosis of the femoral head in Sprague-Dawley rats［J］.J Bone Miner Metab，2020，38（6）：780-793.doi：10.1007/s00774-020-01114-0http://dx.doi.org/10.1007/s00774-020-01114-0.

魏波，王黎明，徐燕，等.股骨头髓芯减压为基础治疗早期股骨头坏死［J］.中国组织工程研究，2012，16（39）：7390-7394.doi：10.3969/j.issn.2095-4344.2012.39.034http://dx.doi.org/10.3969/j.issn.2095-4344.2012.39.034.

庄传记，陈文昭，江新民.骨形态发生蛋白9复合胶原基骨修复材料体外成骨性能与体内修复骨缺损［J］.中国组织工程研究，2021，25（10）：1489-1494.

李大鹏，沈铁城，黄永辉，等.兔骨髓间充质干细胞与纳米晶胶原基骨修复材料体外构建组织工程骨的实验研究［J］.江苏大学学报（医学版），2007，17（5）：389-392.doi：10.3969/j.issn.1671-7783.2007.05.007http://dx.doi.org/10.3969/j.issn.1671-7783.2007.05.007.

Chao PC，Cui MY，Li XA，et al.Correlation between miR-1207-5p expression with steroid-induced necrosis of femoral head and VEGF expression［J］.Eur Rev Med Pharmacol Sci，2019，23（7）：2710-2718.doi：10.26355/eurrev_201904_17541http://dx.doi.org/10.26355/eurrev_201904_17541.

Clarkin CE，Emery RJ，Pitsillides AA，et al.Evaluation of VEGF-mediated signaling in primary human cells reveals a paracrine action for VEGF in osteoblast-mediated crosstalk to endothelial cells［J］.J Cell Physiol，2008，214（2）：537-544.doi：10.1002/jcp.21234http://dx.doi.org/10.1002/jcp.21234.

Dai J，Rabie AB.VEGF：an essential mediator of both angiogenesis and endochondral ossification［J］.J Dent Res，2007，86（10）：937-950.doi：10.1177/154405910708601006http://dx.doi.org/10.1177/154405910708601006.

Luo Y，Li D，Xie X，et al.Porous，lithium-doped calcium polyphosphate composite scaffolds containing vascular endothelial growth factor （VEGF）-loaded gelatin microspheres for treating glucocorticoid-induced osteonecrosis of the femoral head［J］.Biomed Mater，2019，14（3）：035013.doi：10.1088/1748-605X/ab0a55http://dx.doi.org/10.1088/1748-605X/ab0a55.

陈东，华文彬，叶树楠，等.骨内注射无水乙醇建立兔股骨头坏死模型［J］.中国组织工程研究，2013，17（2）：205-209.doi：10.3969/j.issn.2095-4344.2013.02.004http://dx.doi.org/10.3969/j.issn.2095-4344.2013.02.004.

Aruwajoye OO，Patel MK，Allen MR，et al.Microcrack density and nanomechanical properties in the subchondral region of the immature piglet femoral head following ischemic osteonecrosis［J］.Bone，2013，52（2）：632-639.doi：10.1016/j.bone.2012.07.028http://dx.doi.org/10.1016/j.bone.2012.07.028.

Gou WL，Lu Q，Wang X，et al.Key pathway to prevent the collapse of femoral head in osteonecrosis［J］.Eur Rev Med Pharmacol Sci，2015，19（15）：2766-2774.

Erken HY，Ofluoglu O，Aktas M，et al.Effect of pentoxifylline on histopathological changes in steroid-induced osteonecrosis of femoral head：experimental study in chicken［J］.Int Orthop，2012，36（7）：1523-1528.doi：10.1007/s00264-012-1497-6http://dx.doi.org/10.1007/s00264-012-1497-6.

Marenzana M，Arnett TR.The key role of the blood supply to bone［J］.Bone Res，2013，1（3）：203-215.doi：10.4248/BR201303001http://dx.doi.org/10.4248/BR201303001.

Hu K，Olsen BR.The roles of vascular endothelial growth factor in bone repair and regeneration［J］.Bone，2016，91：30-38.doi：10.1016/j.bone.2016.06.013http://dx.doi.org/10.1016/j.bone.2016.06.013.

Grosso A，Burger MG，Lunger A，et al.It takes two to tango：coupling of angiogenesis and osteogenesis for bone regeneration［J］.Front Bioeng Biotechnol，2017，5：68.doi：10.3389/fbioe.2017.00068http://dx.doi.org/10.3389/fbioe.2017.00068.

Hang D，Wang Q，Guo C，et al.Treatment of osteonecrosis of the femoral head with VEGF165 transgenic bone marrow mesenchymal stem cells in mongrel dogs［J］.Cells Tissues Organs，2012，195（6）：495-506.doi：10.1159/000329502http://dx.doi.org/10.1159/000329502.

Hong GJ，Lin N，Chen LL，et al.Association between vascular endothelial growth factor gene polymorphisms and the risk of osteonecrosis of the femoral head：systematic review［J］.Biomed Rep，2016，4（1）：92-96.doi：10.3892/br.2015.527http://dx.doi.org/10.3892/br.2015.527.

Apte RS，Chen DS，Ferrara N.VEGF in signaling and disease：beyond discovery and development［J］.Cell，2019，176（6）：1248-1264.doi：10.1016/j.cell.2019.01.021http://dx.doi.org/10.1016/j.cell.2019.01.021.

Gerber HP，Vu TH，Ryan AM，et al.VEGF couples hypertrophic cartilage remodeling，ossification and angiogenesis during endochondral bone formation［J］.Nat Med，1999，5（6）：623-628.doi：10.1038/9467http://dx.doi.org/10.1038/9467.

Midy V，Plouët J.Vasculotropin/vascular endothelial growth factor induces differentiation in cultured osteoblasts［J］.Biochem Biophys Res Commun，1994，199（1）：380-386.doi：10.1006/bbrc. 1994.1240http://dx.doi.org/10.1006/bbrc.1994.1240.

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