Correlation between serum miR-375 and CHSY1 expression and helicobacter pylori infection in gastric cancer

CAI Zhan-huan; CHEN Sheng; WANG Yan-liang

doi:10.11659/jjssx.10E022011

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Correlation between serum miR-375 and CHSY1 expression and helicobacter pylori infection in gastric cancer

Clinical Research | 更新时间：2023-07-17

- Correlation between serum miR-375 and CHSY1 expression and helicobacter pylori infection in gastric cancer
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 7, Pages: 604-606(2023)
- 作者机构：
  
  儋州市人民医院/海南医学院附属儋州医院普通外科，海南儋州 571700
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.10E022011
  CLC： R446;R573.3+2
- Published：25 July 2023，
  
  Received：08 October 2022，
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蔡沾焕,陈升,王艳良.胃癌血清miR-375和CHSY1表达与幽门螺杆菌感染的相关性研究[J].局解手术学杂志,2023,32(07):604-606.

CAI Zhan-huan,CHEN Sheng,WANG Yan-liang.Correlation between serum miR-375 and CHSY1 expression and helicobacter pylori infection in gastric cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(07):604-606.
蔡沾焕,陈升,王艳良.胃癌血清miR-375和CHSY1表达与幽门螺杆菌感染的相关性研究[J].局解手术学杂志,2023,32(07):604-606. DOI： 10.11659/jjssx.10E022011.

CAI Zhan-huan,CHEN Sheng,WANG Yan-liang.Correlation between serum miR-375 and CHSY1 expression and helicobacter pylori infection in gastric cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(07):604-606. DOI： 10.11659/jjssx.10E022011.

摘要

目的

探讨胃癌血清miR-375和硫酸软骨素酶1（CHSY1）表达水平与幽门螺杆菌（Hp）感染的相关性。

方法

选取2018年6月至2021年6月我院收治的胃癌患者96例设为胃癌组，另选取同期在我院就诊的慢性浅表性胃炎患者68例设为对照组。采用

C呼气试验检测Hp感染情况，根据Hp的感染密度将患者分为Hp轻度组（25例）、Hp中度组（30例）、Hp重度组（26例）。采用qPT-PCR测定血清中miR-375和CHSY1 mRNA表达水平。

Spearman

相关性分析血清中miR-375和CHSY1表达水平与胃癌患者Hp感染的相关性。

结果

与对照组相比，胃癌组Hp感染阳性率显著较高(

0.05)，Hp阴性及Hp阳性患者血清中miR-375表达水平均显著降低(

0.05)，CHSY1 mRNA表达水平均显著升高(

0.05)。胃癌组及对照组Hp阳性患者血清中miR-375 表达水平低于Hp阴性患者，CHSY1 mRNA表达水平高于Hp阴性患者(

0.05)。随着Hp的感染程度增加，miR-375的表达水平呈降低趋势(

0.05)，CHSY1 mRNA的表达水平呈升高趋势(

0.05)。

Spearman

相关性分析结果显示，血清中miR-375表达水平与胃癌患者Hp感染呈负相关（

-0.648，

0.001），CHSY1 mRNA表达水平与胃癌患者Hp感染呈正相关（

0.534，

0.001）。

结论

胃癌患者血清中miR-375表达水平降低，CHSY1表达水平升高，且与Hp感染密切相关。

Abstract

Objective

To investigate the correlation between serum miR-375 and chondroitin sulfate synthase 1 （CHSY1） expression levels and helicobacter pylori （Hp） infection in gastric cancer.

Methods

A total of 96 patients with gastric cancer admitted to our hospital from June 2018 to June 2021 were selected as the gastric cancer group， and 68 patients with chronic superficial gastritis admitted to our hospital during the same period were selected as the control group. The

C breath test was performed to detect the Hp infection. According to the Hp infection density， patients were divided into the Hp mild group （25 cases）， the Hp moderate group （30 cases）， and the Hp severe group （26 cases）. The expression levels of miR-375 and CHSY1 mRNA in serum were determined by qPT-PCR.

Spearman

correlation was applied to analyze the correlation between the expression levels of miR-375 and CHSY1 in serum and Hp infection in patients with gastric cancer.

Results

Compared with the control group， the positive rate of Hp infection in the gastric cancer group was significantly higher （

0.05）， the expression levels of miR-375 in serum of HP-negative and HP-positive patients were significantly decreased （

0.05）， and the expression levels of CHSY1 mRNA were significantly increased （

0.05）. The expression levels of miR-375 in serum of Hp-positive patients in the gastric cancer group and the control group were lower than that of Hp-negative patients （

0.05）， and the expression levels of CHSY1 mRNA were higher than that of Hp-negative patients （

0.05）. With the increase of the degree of Hp infection， the expression level of miR-375 decreased and the expression level of CHSY1 mRNA increased （

0.05）. The results of

Spearman

correlation analysis showed that the expression level of miR-375 in serum was negatively correlated with Hp infection in patients with gastric cancer （

=-0.648，

0.001）， and the expression level of CHSY1 mRNA was positively correlated with Hp infection in patients with gastric cancer （

=0.534，

0.001）.

Conclusion

The expression level of miR-375 in serum of patients with gastric cancer decreases， and the expression level of CHSY increases， both of which are closely related to Hp infection.

关键词

幽门螺杆菌胃癌miR-375硫酸软骨素酶1

Keywords

helicobacter pylorigastric cancermiR-375chondroitin sulfate synthase 1

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