Mechanism of down-regulation of MAC30 on pyrophosis,angiogenesis and ATP1A2 protein in breast cancer cells

PAN Lei-lei; MA Jun; YE Ting-ting; CHEN Jian-ping

doi:10.11659/jjssx.09E022120

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Mechanism of down-regulation of MAC30 on pyrophosis,angiogenesis and ATP1A2 protein in breast cancer cells

Basic Research | 更新时间：2023-06-13

- Mechanism of down-regulation of MAC30 on pyrophosis,angiogenesis and ATP1A2 protein in breast cancer cells
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 6, Pages: 481-486(2023)
- 作者机构：
  
  1.宣城中心医院心胸乳腺外科，安徽宣城 242000
  2.皖南医学院弋矶山医院甲乳外科，安徽芜湖 241000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.09E022120
  CLC： R737.9
- Published：25 June 2023，
  
  Received：22 September 2022，
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潘蕾蕾,马俊,叶亭亭等.下调MAC30对乳腺癌细胞焦亡、血管生成及ATP1A2蛋白的作用机制[J].局解手术学杂志,2023,32(06):481-486.

PAN Lei-lei,MA Jun,YE Ting-ting,et al.Mechanism of down-regulation of MAC30 on pyrophosis,angiogenesis and ATP1A2 protein in breast cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(06):481-486.
潘蕾蕾,马俊,叶亭亭等.下调MAC30对乳腺癌细胞焦亡、血管生成及ATP1A2蛋白的作用机制[J].局解手术学杂志,2023,32(06):481-486. DOI： 10.11659/jjssx.09E022120.

PAN Lei-lei,MA Jun,YE Ting-ting,et al.Mechanism of down-regulation of MAC30 on pyrophosis,angiogenesis and ATP1A2 protein in breast cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(06):481-486. DOI： 10.11659/jjssx.09E022120.

摘要

目的

探讨下调MAC30对乳腺癌细胞焦亡、血管生成及ATP1A2蛋白水平的影响。

方法

MCF-7细胞制成单个细胞悬液，分为乳腺癌组（MCF-7细胞）、NC组（MCF-7细胞转染MAC30-NC-shRNA）、MAC30 shRNA组（MCF-7细胞转染MAC30-shRNA）、ATP1A2组（MCF-7细胞转染ATP1A2-shRNA）及MAC30 shRNA+ATP1A2组（MCF-7细胞转染MAC30-shRNA、ATP1A2-shRNA）。qRT-PCR检测各组MCF-7细胞中MAC30 mRNA表达以证实转染效率，采用CCK-8法检测细胞增殖活性；流式细胞仪检测细胞焦亡率；体外血管形成实验检测血管形成数目；Western blot检测ATP1A2、SCr、AKT及PI3K水平。

结果

乳腺癌组与NC组MCF-7细胞中MAC30 mRNA表达水平、MCF-7细胞焦亡率及MCF-7细胞血管形成数目比较，差异均无统计学意义（

0.05）。与乳腺癌组和NC组比较，MAC30 shRNA组、ATP1A2组及MAC30 shRNA+ATP1A2组MCF-7细胞中MAC30 mRNA表达水平及血管形成数目降低，MCF-7细胞焦亡率升高，差异有统计学意义（

0.05），其中MAC30 shRNA+ATP1A2组降低/升高最明显（

0.05）。与NC组相比，MAC30 shRNA组、ATP1A2组及MAC30 shRNA+ATP1A2组细胞增殖活性均降低（

0.05）。与NC组相比，MAC30 shRNA组ATP1A2、SCr、AKT及PI3K表达水平降低（

0.05）。

结论

下调MAC30可以抑制乳腺癌细胞增殖及血管生成，加快焦亡，其机制与抑制ATP1A2蛋白表达相关。

Abstract

Objective

To explore the effect of down-regulation of MAC30 on pyrophosis， angiogenesis and ATP1A2 protein level in breast cancer cells.

Methods

MCF-7 cells were made into single cell suspension and divided into the breast cancer group （MCF-7 cells）， the NC group （MCF-7 cells transfected with MAC30-NC-shRNA）， the MAC30 shRNA group （MCF-7 cells transfected with MAC30-shRNA）， the ATP1A2 group （MCF-7 cells transfected with ATP1A2-shRNA） and the MAC30 shRNA +ATP1A2 group （MCF-7 cells transfected with MAC30-shRNA and ATP1A2-shRNA）. qRT-PCR was used to detect the MAC30 mRNA expression in MCF-7 cells of each group to verify the transfection efficiency. CCK-8 assay was used to detect the cell proliferation activity. Flow cytometry was used to detect the pyrophosis rate. In vitro angiogenesis experiment was used to detect the number of angiogenesis. Western blot was used to detect the levels of ATP1A2， SCr， AKT and PI3K.

Results

There was no statistically significant difference in the MAC30 mRNA expression level in MCF-7 cells， the pyrophosis rate of MCF-7 cells or the number of angiogenesis in MCF-7 cells between the breast cancer group and the NC group （

0.05）. Compared with the breast cancer group and the NC group， the MAC30 mRNA expression levels and the number of angiogenesis in MCF-7 cells of the MAC30 shRNA group， the ATP1A2 group， and the MAC30 shRNA+ATP1A2 group were decreased， and the pyrophosis rate in MCF-7 cells was increased， with statistical significance （

0.05）， of which the MAC30 shRNA+ATP1A2 group decreased/increased most significantly （

0.05）. Compared with the NC group， the cell proliferative activities of the MAC30 shRNA group， the ATP1A2 group， and the MAC30 shRNA+ATP1A2 group were decreased （

0.05）. Compared with the NC group， the expression levels of ATP1A2， SCr， AKT， and PI3K of the MAC30 shRNA group were decreased （

0.05）.

Conclusion

Down-regulation of MAC30 can inhibit the proliferation and angiogenesis of breast cancer cells and accelerate pyroptosis， which may be related to the inhibition of ATP1A2 protein expression.

关键词

乳腺癌脑膜瘤相关蛋白焦亡血管生成Na+-K+-ATP酶α2亚基基因

Keywords

breast cancermeningioma-associated proteinpyroptosisangiogenesisNa+-K+-ATPase α2 subunit gene

references

Neemat K，Hebatallah K，Loay K，et al.Detection of activating mutations in liquid biopsy of Egyptian breast cancer patients using targeted next-generation sequencing：a pilot study［J］.J Egypt Natl Canc Inst，2021，33（1）：10.doi：10.1186/s43046-021-00067-3http://dx.doi.org/10.1186/s43046-021-00067-3.

Zhou J，Zhu X，Wu S，et al.Epithelial-mesenchymal transition status of circulating tumor cells in breast cancer and its clinical relevance［J］.Cancer Biol Med，2020，17（1）：169-180.doi：10.20892/j.issn.2095-3941.2019.0118http://dx.doi.org/10.20892/j.issn.2095-3941.2019.0118.

Lee D，Lee YH，Lee KH，et al.Aviculin isolated from lespedeza cuneata induce apoptosis in breast cancer cells through mitochondria-mediated caspase activation pathway［J］.Molecules，2020，25（7）：1708.doi：10.3390/molecules25071708http://dx.doi.org/10.3390/molecules25071708.

Zou G，Zhang X，Wang L，et al.Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA ranscription［J］.Theranostics，2020，10（15）：6839-6853.doi：10.7150/thno. 43622http://dx.doi.org/10.7150/thno.43622.

Razak NA，Abu N，Ho WY，et al.Cytotoxicity of eupatorin in MCF-7 and MDA-MB-231 human breast cancer cells via cell cycle arrest， anti-angiogenesis and induction of apoptosis［J］.Sci Rep，2019，9（1）：1514.doi：10.1038/s41598-018-37796-whttp://dx.doi.org/10.1038/s41598-018-37796-w.

覃绎，袁洪范，曾蓓蕾，等.ATP1A2通过Src/PI3K/Akt信号通路抑制人乳腺癌细胞的侵袭和迁移［J］.第三军医大学学报，2019，41（22）：2166-2173.doi：10.16016/j.1000-5404.201906041http://dx.doi.org/10.16016/j.1000-5404.201906041.

Wu X，Zhang Y，Guo J，et al.MAC30 knockdown inhibits proliferation and enhance apoptosis of gastric cancer by suppressing wnt/β-cateninsignaling pathway［J］.Gastroenterol Res Pract，2020，20（17）：1-11.doi：10.1155/2020/6358685http://dx.doi.org/10.1155/2020/6358685.

Foukakis T，Von Minckwitz G，Bengtsson NO，et al.Cyclin E1 （CCNE1） and E2 （CCNE2） as prognostic and predictive markers for endocrine therapy （ET） in early breast cancer［J］.JAMA，2020，316（18）：1888.

Zhang Y，Li H，Wang J，et al.Meningioma-associated protein 30 accelerates the proliferation and invasion of hepatocellular carcinoma by modulating Wnt/GSK-3β/β-catenin signaling［J］.J Bioenerg Biomembr，2021，53（1）：73-83.doi：10.1007/s10863-020-09864-2http://dx.doi.org/10.1007/s10863-020-09864-2.

Song GQ，Yi Z.MAC30 knockdown involved in the activation of the Hippo signaling pathway in breast cancer cells［J］.Biol Chem，2018，399（11）：1305-1311.doi：10.1515/hsz-2018-0250http://dx.doi.org/10.1515/hsz-2018-0250.

Kayed H，Kleeff J，Ding J，et al.Expression analysis of MAC30 in human pancreatic cancer and tumors of the gastrointestinal tract［J］.Histol Histopathol，2004，19（4）：1021-1031.doi：10.14670/HH-19.1021http://dx.doi.org/10.14670/HH-19.1021.

Yang S，Li H，Liu Y，et al.Elevated expression of MAC30 predicts lymph node metastasis and unfavorable prognosis in patients with epithelial ovarian cancer［J］.Med Oncol，2013，30（1）：324.doi：10.1007/s12032-012-0324-7http://dx.doi.org/10.1007/s12032-012-0324-7.

陈毅翀，赵毅.敲减MAC30通过PI3K/AKT抑制人乳腺癌细胞增殖并诱导其凋亡［J］.现代肿瘤医学，2019，27（18）：3167-3171.doi：10.3969/j.issn.1672-4992.2019.18.001http://dx.doi.org/10.3969/j.issn.1672-4992.2019.18.001.

Xu XY，Zhang LJ，et al.Down-regulated MAC30 expression inhibits proliferation and mobility of human gastric cancer cells［J］.Cell Physiol Biochem，2014，33（5）：1359-1368.doi：10.1159/0003 58703http://dx.doi.org/10.1159/000358703.

乔恩奇，陈道宝，杨红健.miR-150基于PI3K/Akt信号通路调控乳腺癌细胞生物学特性的研究［J］.中国妇幼保健，2019，34（16）：3805-3809.doi：10.7620/zgfybj.j.issn.1001-4411.2019.16.56http://dx.doi.org/10.7620/zgfybj.j.issn.1001-4411.2019.16.56.

张慧敏，刘晓旭，谢佩玲，等.盐酸青藤碱通过抑制bFGF介导的乳腺癌血管新生发挥抑瘤作用［J］.西安交通大学学报（医学版），2021，42（6）：947-952.doi：10.7652/jdyxb202106026http://dx.doi.org/10.7652/jdyxb202106026.

Zhao S，Geng Y，Cao L，et al.Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway［J］.Am J Cancer Res，2021，11（5）：2142-2158.

Asemani S，Montazeri V，Foroutan-Ghaznavi M，et al.Dietary patterns and relative expression levels of PPAR-γ，VEGF-A and HIF-1α genes in benign breast diseases：case-control and consecutive case-series designs［J］.Br J Nutr，2020，124（8）：832-843.doi：10.1017/S0007114520001737http://dx.doi.org/10.1017/S0007114520001737.

陈毅翀.MAC30通过PI3K/AKT促进人乳腺癌细胞的增殖并抑制凋亡［D］.沈阳：中国医科大学.2019.doi：10.27652/d.cnki.gzyku.2019.000326http://dx.doi.org/10.27652/d.cnki.gzyku.2019.000326.

徐其锋，温晓琼.竹叶提取物通过调控Akt/HIF-1α通路抑制低氧条件下人乳腺癌MCF-7细胞的恶性生长［J］.西安交通大学学报（医学版），2018，39（1）：121-126.doi：10.7652/jdyxb201801026http://dx.doi.org/10.7652/jdyxb201801026.

Bogdanov A，Moiseenko F，Dubina M.Abnormal expression of ATP1A1 and ATP1A2 in breast cancer［J］.F1000Res，2017，6：10.doi：10.12688/f1000research.10481.1http://dx.doi.org/10.12688/f1000research.10481.1.

Chen F，Li Y，Qin N，et al.RNA-seq analysis identified hormone-related genes associated with prognosis of triple negative breast cancer［J］.J Biomed Res.2020 Jan 29；34（2）：129-138.doi：10.7555/JBR.34.20190111http://dx.doi.org/10.7555/JBR.34.20190111.

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