Expressions of cytokines and procalcitonin in infective endocarditis

WANG Ruo-xin; FU Liang; ZHAO Jin-long; CHEN Zong-hui; NI Yin-kai; LI Feng

doi:10.11659/jjssx.08E023079

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Expressions of cytokines and procalcitonin in infective endocarditis

Clinical Research | 更新时间：2024-01-22

- Expressions of cytokines and procalcitonin in infective endocarditis
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 33, Issue 1, Pages: 55-58(2024)
- 作者机构：
  
  上海市第六人民医院心血管外科，上海 200233
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.08E023079
  CLC： R654.2
- Published：25 January 2024，
  
  Received：20 August 2023，
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王若昕,付亮,赵金龙等.感染性心内膜炎细胞因子和降钙素原的表达[J].局解手术学杂志,2024,33(01):55-58.

WANG Ruo-xin,FU Liang,ZHAO Jin-long,et al.Expressions of cytokines and procalcitonin in infective endocarditis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):55-58.
王若昕,付亮,赵金龙等.感染性心内膜炎细胞因子和降钙素原的表达[J].局解手术学杂志,2024,33(01):55-58. DOI： 10.11659/jjssx.08E023079.

WANG Ruo-xin,FU Liang,ZHAO Jin-long,et al.Expressions of cytokines and procalcitonin in infective endocarditis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):55-58. DOI： 10.11659/jjssx.08E023079.

摘要

目的

探讨感染性心内膜炎（IE）患者中12项细胞因子（IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IL-12p70、IL-17、IFN-α、IFN-γ、TNF-α）及降钙素原的表达。

方法

纳入我院2021年12月至2022年12月IE患者10例为IE组，随机选择同期入院手术的非感染性、非风湿性瓣膜病的心内膜炎患者10例为对照组，患者均于入院时采血。采用流式细胞术检测12种细胞因子的表达及血常规指标，采用ELISA法检测降钙素原水平，采用

Pearson

法分析IE患者细胞因子表达水平间的相关性，

Spearman

法分析IE患者IL-8水平、白细胞计数与降钙素原的相关性。

结果

与对照组比较，IE组患者中细胞因子IL-1β、IL-2、IL-6、IL-10、TNF-α、IFN-α、IFN-γ、IL-12p70显著升高（

0.05），白细胞计数、中性粒细胞百分比、降钙素原水平显著升高（

0.05）。2组患者单核细胞百分比比较，差异无统计学意义（

0.05）。IE组患者中的IFN-α与IL-2、TNF-α、IL-1β、IL-12p70呈正相关；IL-2与TNF-α、IL-1β呈正相关；IL-12p70与IFN-γ呈正相关；降钙素原与IL-8、白细胞计数呈显著正相关，差异均有统计学意义（

0.05）。

结论

IE患者中IL-1β、IL-2、IL-6、IL-10、TNF-α、IFN-α、IFN-γ、IL-12p70、降钙素原等水平明显高于正常人群，这些指标的检测对于IE的早期诊断及病情严重程度的评估具有指导意义。

Abstract

Objective

To investigate the expressions of 12 cytokines （IL-1β， IL-2， IL-4， IL-5， IL-6， IL-8， IL-10， IL-12p70， IL-17， IFN-α， IFN-γ， TNF-α） and procalcitonin in patients with infective endocarditis （IE）.

Methods

Ten IE patients admitted to our hospital from December 2021 to December 2022 were included into the IE group，10 patients with non-infectious and non-rheumatic valvular diseases who were admitted to our hospital at the same period were randomly selected as the control group， and blood sampling of all patients were conducted at admission. The expressions of 12 cytokines and blood routine indexes were detected by flow cytometry， and the level of procalcitonin was detected by ELISA. The correlations among the expression levels of cytokines in IE patients were analyzed by

Pearson

method and the correlations of IL-8 level and white blood cell count with procalcitonin in IE patients were analyzed by

Spearman

method.

Results

Compared with the control group，the levels of cytokines of IL-1β，IL-2，IL-6，IL-10，TNF-α，IFN-α，IFN-γ and IL-12p70 in the IE group were significantly increased （

0.05）， the white blood cell count，neutrophil percentage and procalcitonin were significantly increased （

0.05）. There was no significant difference in the percentage of monocytes between the two groups（

0.05）.IFN-α of IE patients was positively correlated with IL-2，TNF-α，IL-1β and IL-12p70，IL-2 was positively correlated with TNF-α and IL-1β，IL-12p70 was positively correlated with IFN-γ， and procalcitonin was significantly positively correlated with IL-8 and white blood cell count， with statistically significant differences （

0.05）.

Conclusion

The levels of IL-1β， IL-2， IL-6， IL-10， TNF-α， IFN-α， IFN-γ， IL-12p70 and procalcitonin in IE patients are significantly higher than those in the normal population， and the detections of these indicators are of guiding significance for the early diagnosis of IE and the evaluation of the severity of the disease.

关键词

感染性心内膜炎细胞因子降钙素原心脏瓣膜病血常规检查

Keywords

infective endocarditiscytokinesprocalcitoninvalvular heart diseaseblood routine examination

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