Rosmarinic acid inhibits the proliferation and migration of laryngeal carcinoma cells through the PI3K/AKT/mTOR signaling pathway

YAN Qing; YANG Hua-rong; WANG Na-na

doi:10.11659/jjssx.08E022111

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Rosmarinic acid inhibits the proliferation and migration of laryngeal carcinoma cells through the PI3K/AKT/mTOR signaling pathway

Basic Research | 更新时间：2023-09-26

- Rosmarinic acid inhibits the proliferation and migration of laryngeal carcinoma cells through the PI3K/AKT/mTOR signaling pathway
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 9, Pages: 778-782(2023)
- 作者机构：
  
  延安大学附属医院鼻咽喉科，陕西延安716000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.08E022111
  CLC： R739.65
- Published：25 September 2023，
  
  Received：19 August 2022，
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延青,杨花荣,王娜娜.迷迭香酸通过PI3K/AKT/mTOR信号通路抑制喉癌细胞的增殖和迁移[J].局解手术学杂志,2023,32(09):778-782.

YAN Qing,YANG Hua-rong,WANG Na-na.Rosmarinic acid inhibits the proliferation and migration of laryngeal carcinoma cells through the PI3K/AKT/mTOR signaling pathway[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(09):778-782.
延青,杨花荣,王娜娜.迷迭香酸通过PI3K/AKT/mTOR信号通路抑制喉癌细胞的增殖和迁移[J].局解手术学杂志,2023,32(09):778-782. DOI： 10.11659/jjssx.08E022111.

YAN Qing,YANG Hua-rong,WANG Na-na.Rosmarinic acid inhibits the proliferation and migration of laryngeal carcinoma cells through the PI3K/AKT/mTOR signaling pathway[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(09):778-782. DOI： 10.11659/jjssx.08E022111.

摘要

目的

探讨迷迭香酸（RA）对喉鳞状细胞癌（LSCC）细胞增殖、迁移的影响，并分析其潜在机制。

方法

取对数生长期的Hep-2和TU686细胞，分为对照组（未处理的Hep-2和TU686细胞）、低剂量组（20 μmol/L的RA处理）、中剂量组（50 μmol/L的RA处理）、高剂量组（100 μmol/L的RA处理）、胰岛素生长因子1（IGF-1）组（10 nmol/mL IGF-1处理）、IGF-1+RA组（100 μmol/L的RA与10 nmol/mL IGF-1同时处理）。倒置显微镜观察各组Hep-2和TU686细胞形态；CCK-8法检测各组Hep-2和TU686细胞增殖能力；细胞划痕实验检测各组Hep-2和TU686细胞迁移能力；Western blot检测各组Hep-2和TU686细胞中Ki-67、MMP-2、MMP-9及PI3K/AKT/mTOR信号通路相关蛋白的表达。

结果

RA可显著抑制Hep-2和TU686细胞的增殖、迁移，降低Ki-67、MMP-2、MMP-9蛋白表达和p-PI3K/PI3K、p-AKT/AKT、p-mTOR/mTOR水平（

0.05）；IGF-1可有效逆转RA对Hep-2和TU686细胞增殖、迁移以及PI3K/AKT/mTOR信号通路的抑制作用（

0.05）。

结论

RA可能通过PI3K/AKT/mTOR信号通路抑制LSCC细胞的增殖和迁移。

Abstract

Objective

To investigate the effect of rosmarinic acid （RA） on the proliferation and migration of laryngeal squamous cell carcinoma （LSCC） cells， and analyze its underlying mechanism.

Methods

Hep-2 and TU686 cells in logarithmic growth phase were taken and divided into control group （untreated Hep-2 and TU686 cells），low-dose group （treated with 20 μmol/L RA），middle-dose group （treated with 50 μmol/L RA），high-dose group （treated with 100 μmol/L RA），insulin-like growth factor 1 （IGF-1） group （treated with 10 nmol/L IGF-1） and IGF-1+RA group （co-treated with 100 μmol/L RA and 10 nmol/mL IGF-1）.Inverted microscope was used to observe the morphology of Hep-2 and TU686 cells in each group； CCK-8 method was used to detect the proliferation of Hep-2 and TU686 cells in each group； scratch wound healing assay was used to detect the migration ability of Hep-2 and TU686 cells in each group； Western blot was used to detect the expression of Ki-67， MMP-2， MMP-9 and PI3K/pAKT/mTOR signaling pathway-related proteins in Hep-2 and TU686 cells of each group.

Results

RA could significantly inhibit the proliferation and migration of Hep-2 and TU686 cells， reduce the protein expression of Ki-67， MMP-2， MMP-9 and the levels of p-PI3K/PI3K， p-AKT/AKT， and p-mTOR/mTOR （

0.05）； IGF-1 could effectively reverse the inhibitory effect of RA on Hep-2 and TU686 cell proliferation， migration and PI3K/AKT/mTOR signaling pathway （

0.05）.

Conclusion

RA may inhibit the proliferation and migration of LSCC cells through PI3K/AKT/mTOR signaling pathway.

关键词

迷迭香酸PI3K/AKT/mTOR信号通路喉鳞状细胞癌细胞增殖细胞迁移

Keywords

rosmarinic acidPI3K/AKT/mTOR signaling pathwaylaryngeal squamous cell carcinomacell proliferationcell migration

references

Vander Poorten V， Meulemans J， Beitler JJ， et al. Salvage surgery for residual or recurrent laryngeal squamous cell carcinoma after （Chemo）radiotherapy： oncological outcomes and prognostic factors［J］. Eur J Surg Oncol， 2021， 47（11）：2711-2721. doi： 10.1016/j.ejso.2021.05.035http://dx.doi.org/10.1016/j.ejso.2021.05.035.

Lee NY， Ferris RL， Psyrri A， et al. Avelumab plus standard-of-care chemoradiotherapy versus chemoradiotherapy alone in patients with locally advanced squamous cell carcinoma of the head and neck： a randomised， double-blind， placebo-controlled， multicentre， phase 3 trial［J］. Lancet Oncol， 2021， 22（4）：450-462. doi： 10.1016/S1470-2045（20）30737-3http://dx.doi.org/10.1016/S1470-2045（20）30737-3.

Liu Y， Song Y， Chen X， et al. miR-206 inhibits laryngeal carcinoma cell multiplication， migration， and invasion［J］. J Healthc Eng， 2021（1）：5614861. doi： 10.1155/2021/5614861http://dx.doi.org/10.1155/2021/5614861.

Hitl M， Kladar N， Gavarić N， et al. Rosmarinic acid-human pharmacokinetics and health benefits［J］. Planta Med， 2021， 87（4）：273-282. doi： 10.1055/a-1301-8648http://dx.doi.org/10.1055/a-1301-8648.

An Y， Zhao J， Zhang Y， et al. Rosmarinic acid induces proliferation suppression of hepatoma cells associated with NF-κB signaling pathway［J］. Asian Pac J Cancer Prev， 2021， 22（5）：1623-1632. doi： 10.31557/APJCP.2021.22.5.1623http://dx.doi.org/10.31557/APJCP.2021.22.5.1623.

Mahmoud MA， Okda TM， Omran GA， et al. Rosmarinic acid suppresses inflammation， angiogenesis， and improves paclitaxel induced apoptosis in a breast cancer model via NF3 κB-p53-caspase-3 pathways modulation［J］. J Appl Biomed， 2021， 19（4）：202-209. doi： 10.32725/jab.2021.024http://dx.doi.org/10.32725/jab.2021.024.

Wang L， Yang H， Wang C， et al. Rosmarinic acid inhibits proliferation and invasion of hepatocellular carcinoma cells SMMC 7721 via PI3K/AKT/mTOR signal pathway［J］.Biomed Pharmacother，2019，120（1）：109443. doi： 10.1016/j.biopha.2019.109443http://dx.doi.org/10.1016/j.biopha.2019.109443.

Wu H， Wang W， Zhu J. Knockdown of long non-coding RNA RP11-297P16.3 inhibits the migration and invasion of laryngeal squamous carcinoma cells［J］. Clin Transl Oncol， 2021， 23（10）：2057-2065. doi： 10.1007/s12094-021-02609-6http://dx.doi.org/10.1007/s12094-021-02609-6.

卜倩倩，高伟，吴勇延，等. microRNA在喉鳞状细胞癌中的研究进展［J］. 临床耳鼻咽喉头颈外科杂志， 2021， 35（10）：947-951. doi： 10.13201/j.issn.2096-7993.2021.10.019http://dx.doi.org/10.13201/j.issn.2096-7993.2021.10.019.

冯国泰，李阳松，张玲莉. 大黄素通过上调lncRNA MEG3表达并抑制PI3K/AKT信号通路影响喉癌细胞的增殖和凋亡［J］. 中国药师， 2021， 24（8）：463-467. doi： 10.19962/j.cnki.issn1008-049X.2021.08.010http://dx.doi.org/10.19962/j.cnki.issn1008-049X.2021.08.010.

Baranauskaite J， Sadauskiene I， Liekis A， et al. Natural compounds rosmarinic acid and carvacrol counteract aluminium-induced oxidative stress［J］. Molecules， 2020， 25（8）：1807. doi： 10.3390/molecules25081807http://dx.doi.org/10.3390/molecules25081807.

Guo C， Shangguan Y， Zhang M， et al. Rosmarinic acid alleviates ethanol-induced lipid accumulation by repressing fatty acid biosynthesis［J］. Food Funct， 2020， 11（3）：2094-2106. doi： 10.1039/c9fo02357ghttp://dx.doi.org/10.1039/c9fo02357g.

Yang K， Shen Z， Zou Y， et al. Rosmarinic acid inhibits migration， invasion， and p38/AP-1 signaling via miR-1225-5p in colorectal cancer cells［J］. J Recept Signal Transduct Res， 2021， 41（3）：284-293. doi： 10.1080/10799893.2020.1808674http://dx.doi.org/10.1080/10799893.2020.1808674.

Huang L， Chen J， Quan J， et al. Rosmarinic acid inhibits proliferation and migration， promotes apoptosis and enhances cisplatin sensitivity of melanoma cells through inhibiting ADAM17/EGFR/AKT/GSK3β axis［J］. Bioengineered， 2021， 12（1）：3065-3076. doi： 10.1080/21655979.2021.1941699http://dx.doi.org/10.1080/21655979.2021.1941699.

Awan MJ， Gittleman H， Barnholtz-Sloan J， et al. Risk groups of laryngeal cancer treated with chemoradiation according to nomogram scores--a pooled analysis of RTOG 0129 and 0522［J］. Oral Oncol， 2021， 116（10）：105241. doi： 10.1016/j.oraloncology.2021. 105241http://dx.doi.org/10.1016/j.oraloncology.2021.105241.

Song Z， Wang J， Su Q， et al. The role of MMP-2 and MMP-9 in the metastasis and development of hypopharyngeal carcinoma［J］.Braz J Otorhinolaryngol，2021，87（5）：521-528. doi： 10.1016/j.bjorl.2019.10.009http://dx.doi.org/10.1016/j.bjorl.2019.10.009.

张生义，张己为，张旭峰，等. 华蟾素对非小细胞肺癌细胞株A549细胞增殖及PTEN/AKT/mTOR信号通路表达的影响［J］. 蚌埠医学院学报， 2020， 45（9）：1159-1162. doi： 10.13898/j.cnki.issn.1000-2200.2020.09.004http://dx.doi.org/10.13898/j.cnki.issn.1000-2200.2020.09.004.

王雨，罗璨，吉兆宁. 鸦胆子苦素D通过PI3K/Akt信号通路抑制人乳腺癌MDA-MB-231细胞的能量代谢研究［J］. 蚌埠医学院学报， 2020， 45（5）：561-565. doi： 10.13898/j.cnki.issn.1000-2200.2020.05.001http://dx.doi.org/10.13898/j.cnki.issn.1000-2200.2020.05.001.

Wang B， Qin H， Wang Y， et al. Effect of DJ-1 overexpression on the proliferation， apoptosis， invasion and migration of laryngeal squamous cell carcinoma SNU-46 cells through PI3K/AKT/mTOR［J］. Oncol Rep， 2014， 32（3）：1108-1116. doi： 10.3892/or.2014.3286http://dx.doi.org/10.3892/or.2014.3286.

Tan AC. Targeting the PI3K/Akt/mTOR pathway in non-small cell lung cancer （NSCLC）［J］. Thorac Cancer， 2020， 11（3）：511-518. doi： 10.1111/1759-7714.13328http://dx.doi.org/10.1111/1759-7714.13328.

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