Midazolam inhibits proliferation, migration and induces apoptosis of osteosarcoma cells

JI Ying-long; ZHOU Quan-jun; JI Guo-li

doi:10.11659/jjssx.07E022126

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Midazolam inhibits proliferation, migration and induces apoptosis of osteosarcoma cells

Basic Research | 更新时间：2023-07-17

- Midazolam inhibits proliferation, migration and induces apoptosis of osteosarcoma cells
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 7, Pages: 586-591(2023)
- 作者机构：
  
  潍坊市中医院麻醉二科，山东潍坊 261041
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.07E022126
  CLC： R738
- Published：25 July 2023，
  
  Received：30 July 2022，
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季英龙,周全军,冀国力.咪达唑仑抑制骨肉瘤细胞增殖、迁移并诱导其凋亡[J].局解手术学杂志,2023,32(07):586-591.

JI Ying-long,ZHOU Quan-jun,JI Guo-li.Midazolam inhibits proliferation, migration and induces apoptosis of osteosarcoma cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(07):586-591.
季英龙,周全军,冀国力.咪达唑仑抑制骨肉瘤细胞增殖、迁移并诱导其凋亡[J].局解手术学杂志,2023,32(07):586-591. DOI： 10.11659/jjssx.07E022126.

JI Ying-long,ZHOU Quan-jun,JI Guo-li.Midazolam inhibits proliferation, migration and induces apoptosis of osteosarcoma cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(07):586-591. DOI： 10.11659/jjssx.07E022126.

摘要

目的

探讨咪达唑仑对骨肉瘤细胞增殖、凋亡、迁移的影响及其作用机制。

方法

收集我院38例骨肉瘤患者的肿瘤组织和邻近的非肿瘤骨组织样本。体外培养人骨肉瘤细胞系（MG63、U2OS、SAOS2、HOS、143B）和正常成骨细胞系（hFOB1.19）。qRT-PCR检测组织和细胞中circNOL10表达。将U2OS细胞分为对照组、低咪达唑仑组、中咪达唑仑组、高咪达唑仑组、pcDNA-circNOL10组、pcDNA组、咪达唑仑+si-NC组、咪达唑仑+si-circNOL10组。运用流式细胞仪、Transwell、CCK-8和qRT-PCR分别检测U2OS细胞凋亡率、迁移数量、增殖活性以及circNOL10表达水平。

结果

与正常组织和细胞比较，circNOL10在骨肉瘤组织和细胞中的表达显著降低（

0.05）。与对照组比较，低咪达唑仑组、中咪达唑仑组、高咪达唑仑组U2OS细胞凋亡率、抑制率、E-cadherin蛋白水平、circNOL10表达水平显著升高（

0.05），迁移数量、N-cadherin蛋白水平显著减少/降低（

0.05）。与pcDNA组比较，pcDNA-circNOL10组U2OS细胞凋亡率、抑制率、E-cadherin蛋白水平显著升高（

0.05），迁移数量、N-cadherin蛋白水平显著减少/降低（

0.05）。与咪达唑仑+si-NC组比较，咪达唑仑+si-circNOL10组U2OS细胞凋亡率、抑制率、E-cadherin蛋白水平显著降低（

0.05），迁移数量、N-cadherin蛋白水平显著增多/升高（

0.05）。

结论

咪达唑仑通过上调circNOL10表达来诱导骨肉瘤细胞凋亡，并抑制其增殖和迁移。

Abstract

Objective

To investigate the effect of midazolam on the proliferation， apoptosis and migration of osteosarcoma cells and its mechanism.

Methods

The tumor tissue and adjacent non-tumor bone tissue samples of 38 patients with osteosarcoma in our hospital were collected. Human osteosarcoma cell lines （MG63， U2OS， SAOS2， HOS， 143B） and normal osteoblast cell lines （hFOB1.19） were cultured in vitro. qRT-PCR was used to detect the expression of circNOL10 in tissues and cells. U2OS cells were divided into the control group， the low midazolam group， the middle midazolam group， the high midazolam group， the pcDNA-circNOL10 group， the pcDNA group， the midazolam+si-NC group， and the midazolam+si-circNOL10 group. Flow cytometry， Transwell， CCK-8 and qRT-PCR were used to detect the apoptosis rate， migration number， proliferation activity and circNOL10 expression level of U2OS cells.

Results

Compared with normal tissues and cells， the expression of circNOL10 in osteosarcoma tissues and cells was significantly decreased （

0.05）. Compared with the control group， the apoptosis rate， inhibition rate， E-cadherin protein level， and circNOL10 expression level of U2OS cells in the low midazolam group， the middle midazolam group， and the high midazolam group were significantly increased （

0.05）， while the migration number and N-cadherin protein level were significantly reduced （

0.05）. Compared with the pcDNA group， the apoptosis rate， inhibition rate and E-cadherin protein level of U2OS cells in the pcDNA-circNOL10 group were significantly increased （

0.05）， while the migration number and N-cadherin protein level were significantly reduced （

0.05）. Compared with the midazolam+si-NC group， the apoptosis rate， inhibition rate and E-cadherin protein level of U2OS cells in the midazolam+si-circNOL10 group were significantly reduced （

0.05）， while the migration number and N-cadherin protein level were significantly increased （

0.05）.

Conclusion

Midazolam induces apoptosis of osteosarcoma cells， and inhibits its proliferation and migration by up-regulating the expression of circNOL10.

关键词

骨肉瘤咪达唑仑circNOL10增殖迁移凋亡

Keywords

osteosarcomamidazolamcircNOL10proliferationmigrationapoptosis

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