Expression of miR-205 and miR-367 in cutaneous malignant melanoma tissue and their clinical significances

ZHANG Li-jun; YAN Wei-heng; HUANG Guo-qing; SUN Chong-zhou

doi:10.11659/jjssx.05E023043

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Expression of miR-205 and miR-367 in cutaneous malignant melanoma tissue and their clinical significances

Clinical Research | 更新时间：2024-01-22

- Expression of miR-205 and miR-367 in cutaneous malignant melanoma tissue and their clinical significances
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 33, Issue 1, Pages: 84-88(2024)
- 作者机构：
  
  电子科技大学医学院附属绵阳医院/绵阳市中心医院烧伤整形外科，四川绵阳 621000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.05E023043
  CLC： R739.5
- Published：25 January 2024，
  
  Received：10 May 2023，
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张丽君,严伟恒,黄国庆等.皮肤恶性黑色素瘤组织miR-205、miR-367表达及其临床意义[J].局解手术学杂志,2024,33(01):84-88.

ZHANG Li-jun,YAN Wei-heng,HUANG Guo-qing,et al.Expression of miR-205 and miR-367 in cutaneous malignant melanoma tissue and their clinical significances[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):84-88.
张丽君,严伟恒,黄国庆等.皮肤恶性黑色素瘤组织miR-205、miR-367表达及其临床意义[J].局解手术学杂志,2024,33(01):84-88. DOI： 10.11659/jjssx.05E023043.

ZHANG Li-jun,YAN Wei-heng,HUANG Guo-qing,et al.Expression of miR-205 and miR-367 in cutaneous malignant melanoma tissue and their clinical significances[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):84-88. DOI： 10.11659/jjssx.05E023043.

摘要

目的

探讨皮肤恶性黑色素瘤（CMM）组织中miR-205、miR-367的表达及与临床病理特征和预后的关系。

方法

选取2013年5月至2019年12月我院收治的85例CMM患者术中切除的肿瘤组织作为CMM组，另选取同期于本院治疗的80例皮肤良性色素痣患者的痣组织标本作为对照组。采用qRT-PCR法检测miR-205、miR-367的表达水平。收集CMM患者的临床病理资料，分析miR-205、miR-367表达与临床病理特征的关系。

Kaplan-Meier

法绘制患者术后3年的生存曲线，采用

Log-rank

检验分析患者生存率。

COX

比例风险回归模型分析预后的影响因素。

结果

CMM组患者miR-205表达水平低于对照组（

0.05），miR-367表达水平高于对照组（

0.05）。miR-205低表达组患者溃疡、临床分期高、肿瘤侵袭、KPS评分低、原发灶厚、Clark分级高、淋巴结转移的比例高于miR-205高表达组（

0.05），miR-367高表达组患者以上指标比例高于miR-367低表达组（

0.05）。随访3年，中位时间为28.3个月，失访1例。

Kaplan-Meier

生存曲线显示，miR-205高表达组患者3年生存率高于miR-205低表达组（

0.05）；miR-367低表达组患者3年生存率高于miR-367高表达组（

0.05）。

COX

多因素分析显示，临床分期高、KPS评分低、Clark分级高、淋巴结转移及miR-367表达升高为患者预后的危险因素，miR-205表达升高为其保护因素（

0.05）。

结论

CMM组织中miR-205低表达，miR-367高表达，且均与患者溃疡、临床分期高、肿瘤侵袭、KPS评分低、原发灶厚、Clark分级高、淋巴结转移和预后不良有关，可作为CMM患者预后评估的潜在标志物。

Abstract

Objective

To study the expression of miR-205 and miR-367 in cutaneous malignant melanoma （CMM） tissue and their relationships with clinicopathological characteristics and prognosis.

Methods

The tumor tissues from 85 patients with CMM who were admitted to our hospital from May 2013 to December 2019 were selected as the CMM group， and the nevus tissue samples from 80 patients with benign pigmented nevi who were treated in our hospital during the same period were selected as the control group. The expression level of miR-205 and miR-367 of two groups were detected by qRT-PCR. The clinical and pathological data of patients with CMM were collected， and the relationships between the expression of miR-205 and miR-367 and clinicopathological characteristics were analyzed.

Kaplan-Meier

was used to draw the survival curves of patients 3 years after surgery， and the survival rate of patients was analyzed by

Log-rank

test. The influencing factors of prognosis was analyzed by

COX

proportional risk regression model.

Results

The expression level of miR-205 of patients in the CMM group was lower than that in the control group， and the expression level of miR-367 was higher than that in the control group （

0.05）. The proportions of patients with ulceration， high clinical stage，tumor invasion， low KPS score， thick primary lesion， high Clark grade， lymph node metastasis in the miR-205 low-expression group were higher than those in the miR-205 high-expression group（

0.05）， the proportions of patients with the above indexes in the miR-367 high-expression group were higher than those in the miR-367 low-expression group（

0.05）.Patietns were followed up for 3 years， with a median time of 28.3 months， and 1 case was lost to followed-up. The

Kaplan-Meier

survival curve showed that the 3-year survival rate of patients in the miR-205 high-expression group was higher than that in the miR-205 low-expression group （

0.05），and the 3-year survival rate of patients in the miR-367 low-expression group was higher than that in the miR-367 high-expression group （

0.05）.

COX

multivariate regression analysis showed that the high clinical stage， low KPS score， high Clark grade， lymph node metastasis， and high expression of miR-367 were the risk factors for prognosis of patients，while high expression of miR-205 was a protective factor（

0.05）.

Conclusion

Low expression of miR-205 and high expression of miR-367 in CMM tissue are associated with ulceration，high clinical stage，tumor invasion，low KPS score，thick primary lesion，high Clark grade，lymph node metastasis， and poor prognosis. These two indicators may serve as potential biomarkers for prognostic evaluation in CMM patients.

关键词

皮肤恶性黑色素瘤miR-205miR-367临床病理特征预后

Keywords

cutaneous malignant melanomamiR-205miR-367clinicopathological characteristicsprognosis

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