Study on the influencing mechanism of pachymoic acid on myocardial ischemia-reperfusion injury in rats

SANG Jing; LI Lu; JIANG Jie; FAN Wei

doi:10.11659/jjssx.05E023034

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Study on the influencing mechanism of pachymoic acid on myocardial ischemia-reperfusion injury in rats

Basic Research | 更新时间：2023-10-23

- Study on the influencing mechanism of pachymoic acid on myocardial ischemia-reperfusion injury in rats
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 10, Pages: 859-864(2023)
- 作者机构：
  
  1.青岛大学附属青岛市中心医院/青岛市肿瘤医院急诊科，山东青岛 266013
  2.青岛大学附属青岛市中心医院/青岛市肿瘤医院输血科，山东青岛 266013
  3.青岛大学附属青岛市中心医院/青岛市肿瘤医院肝胆血管外科，山东青岛 266013
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.05E023034
  CLC： R543.3
- Published：25 October 2023，
  
  Received：09 May 2023，
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桑晶,李璐,姜洁等.茯苓酸对大鼠心肌缺血再灌注损伤的影响机制研究[J].局解手术学杂志,2023,32(10):859-864.

SANG Jing,LI Lu,JIANG Jie,et al.Study on the influencing mechanism of pachymoic acid on myocardial ischemia-reperfusion injury in rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):859-864.
桑晶,李璐,姜洁等.茯苓酸对大鼠心肌缺血再灌注损伤的影响机制研究[J].局解手术学杂志,2023,32(10):859-864. DOI： 10.11659/jjssx.05E023034.

SANG Jing,LI Lu,JIANG Jie,et al.Study on the influencing mechanism of pachymoic acid on myocardial ischemia-reperfusion injury in rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):859-864. DOI： 10.11659/jjssx.05E023034.

摘要

目的

探究茯苓酸调节Shh/Gli1信号通路对大鼠心肌缺血再灌注损伤的影响。

方法

将SD大鼠分为假手术组、模型组、茯苓酸低剂量组（5 mg/kg）、茯苓酸中剂量组（10 mg/kg）、茯苓酸高剂量组（20 mg/kg）和茯苓酸高剂量(20 mg/kg)+环巴胺（10 mg/kg）组，建立大鼠心肌缺血再灌注损伤模型。TTC染色检测心肌梗死情况；HE染色观察大鼠心肌组织病理学变化；ELISA法检测大鼠血清谷草转氨酶（AST）、肌酸激酶（CK）、心肌肌钙蛋白T（cTnT）水平；TUNEL染色检测心肌细胞凋亡情况；RT-PCR法检测心肌组织Shh、Gli1表达；Western blot检测大鼠心肌组织中Bax、Bcl-2以及Shh/Gli1信号通路相关蛋白表达。

结果

与假手术组相比，模型组大鼠心肌组织有大面积白色梗死区，心肌细胞固缩，形态结构不规则，肌纤维紊乱，有较多炎症细胞浸润，细胞凋亡率升高，血清AST、CK、cTnT水平及Bax表达显著升高（

0.05），Shh、Gli1和Bcl-2蛋白表达显著降低（

0.05）；与模型组相比，不同剂量茯苓酸处理后大鼠梗死面积显著减小，心肌纤维排列稍整齐，炎症细胞浸润明显减少，细胞凋亡率降低，血清AST、CK、cTnT水平及Bax蛋白表达显著降低（

0.05），Shh、Gli1和Bcl-2蛋白表达显著升高（

0.05）；与茯苓酸高剂量组相比，茯苓酸高剂量+环巴胺组大鼠心肌梗死面积明显增大，心肌纤维排列紊乱，炎症细胞浸润明显增多，细胞凋亡率升高，血清AST、CK、cTnT水平及Bax蛋白表达显著升高（

0.05），Shh、Gli1和Bcl-2蛋白表达显著降低（

0.05）。

结论

茯苓酸对心肌缺血再灌注大鼠损伤具有改善作用，可能通过激活Shh/Gli1信号通路发挥作用。

Abstract

Objective

To investigate the impact of pachymoic acid on myocardial ischemia-reperfusion injury in rats by regulating Shh/Gli1 signaling pathway.

Methods

The SD rats were divided into the sham operation group， model group， low dose （5 mg/kg） pachymoic acid group， medium dose （10 mg/kg） pachymoic acid group， high dose （20 mg/kg） pachymoic acid group， and high dose （20 mg/kg） pachymoic acid+cyclopamine （10 mg/kg） group， and the myocardial ischemia-reperfusion model of rats was established. The myocardial infarction was determined by TTC staining，the pathological changes of myocardial tissue in rats was observed by HE staining，the serum levels of aspartate aminotransferase （AST），creatine kinase （CK），and cardiac troponin T （cTnT） were detected by ELISA，the cardiomyocyte apoptosis was detected by TUNEL staining， the expression of Shh and Gli1 of myocardial tissue were detected by RT-PCR； and the expression of Bax， Bcl-2， and Shh/Gli1 signaling pathway related proteins in the rat myocardial tissue were detected by Western blot.

Results

Compared with the sham operation group， there were a large area of white infarction area， cardiomyocytes contraction， irregular morphological structure and muscle fiber disorder of the rat myocardial tissue in the model group， with great inflammatory cell infiltration， a increased apoptosis rate， significantly high levels of serum AST， CK， cTnT and expression of Bax protein（

0.05）， as well as decreased protein expression of Shh，Gli1 and Bcl-2 （

0.05）.Compared with the model group，the infarction area of rats was significantly reduced after treated with different doses of pachymoic acid， the myocardial fibers were arranged slightly neatly， the inflammatory cell infiltration was significantly reduced， with a decreased apoptosis rate， significantly low serum AST， CK， cTnT levels and Bax protein expression （

0.05）， as well as significantly increased protein expression of Shh， Gli1 and Bcl-2 （

0.05）.Compared with the high dose pachymic acid group， the myocardial infarction area of rats in the high-dose poria acid+cyclopamine group was significantly increased， myocardial fiber arrangement was disordered， inflammatory cell infiltration was significantly increased， with a increased apoptosis rate， significantly high serum AST， CK， cTnT levels and Bax protein expression （

0.05）， as well as significantly decreased protein expression of Shh， Gli1 and Bcl-2 （

0.05）.

Conclusion

The pachymoic acid can improve the myocardial ischemia-reperfusion injury in rats， which may be achieved by activating Shh/Gli1 signaling pathway.

关键词

茯苓酸Shh/Gli1信号通路心肌缺血再灌注大鼠

Keywords

pachymoic acidShh/Gli1 signaling pathwaymyocardial ischemia-reperfusionrat

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