Mechanism of estrogen regulating the invasion and migration of endometriosis stromal cells

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Mechanism of estrogen regulating the invasion and migration of endometriosis stromal cells

Basic Research | 更新时间：2023-07-31

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- Mechanism of estrogen regulating the invasion and migration of endometriosis stromal cells
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 8, Pages: 673-678(2023)
- 作者机构：
  
  海南医学院第二附属医院生殖医学科，海南海口 570311
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.05E022110
  CLC： R711.71
- Published：25 August 2023，
  
  Received：30 May 2022，
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廖源,王开举,李浩僡等.雌激素调控子宫内膜异位症基质细胞侵袭和迁移的机制研究[J].局解手术学杂志,2023,32(08):673-678.

LIAO Yuan,WANG Kai-ju,LI Hao-hui,et al.Mechanism of estrogen regulating the invasion and migration of endometriosis stromal cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(08):673-678.
廖源,王开举,李浩僡等.雌激素调控子宫内膜异位症基质细胞侵袭和迁移的机制研究[J].局解手术学杂志,2023,32(08):673-678. DOI： 10.11659/jjssx.05E022110.

LIAO Yuan,WANG Kai-ju,LI Hao-hui,et al.Mechanism of estrogen regulating the invasion and migration of endometriosis stromal cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(08):673-678. DOI： 10.11659/jjssx.05E022110.

摘要

目的

2

探讨雌激素调节长链非编码RNA-339（lncRNA-339）对子宫内膜异位症（EMs）基质细胞侵袭和迁移的作用机制。

方法

2

观察EMs子宫内膜中lncRNA-339的表达。用雌激素受体拮抗剂ICI、白细胞介素-1β（IL-1β）抑制剂ACZ885分别处理经雌二醇（E

2

）刺激的人子宫内膜基质细胞系ThESCs。另外，E

2

处理条件下对ThESCs细胞进行过表达或沉默lncRNA-339，并且过表达lncRNA-339后用ACZ885处理ThESCs细胞。用实时荧光定量PCR（qRT-PCR）法检测lncRNA-339的水平。Western blot检测ThESCs中IL-1β的水平，Transwell法检测ThESCs细胞的迁移率和侵袭率。

结果

2

lncRNA-339在EMs子宫内膜中表达增加（

P

<

0.05）。E

2

或者过表达lncRNA-339都能促进ThESCs细胞中lncRNA-339和IL-1β的表达（

P

<

0.05），促进细胞的迁移和侵袭（

P

<

0.05）。而当抑制E

2

、沉默lncRNA-339及抑制IL-1β时，细胞迁移率和侵袭率降低（

P

<

0.05），IL-1β的表达明显降低（

P

<

0.05）。

结论

2

E

2

刺激lncRNA-339表达升高，并通过上调IL-1β促进EMs基质细胞迁移与侵袭。

Abstract

Objective

2

To investigate the mechanism of estrogen regulating long non-coding RNA-339 （lncRNA-339） on the invasion and migration of stromal cells in endometriosis （EMs）.

Methods

2

The expression of lncRNA-339 in endometrium of EMs was observed. Human endometrial stromal cell lines ThESCs stimulated by estradiol （E

2

） were treated with estrogen receptor antagonists ICI and interleukin-1β （IL-1β） inhibitor ACZ885， respectively. In addition， ThESCs cells were overexpressed or silenced with lncRNA-339 under E

2

treatment， and ThESCs cells were treated with ACZ885 after overexpression of lncRNA-339. The level of lncRNA-339 was detected by quantitative real-time PCR （qRT-PCR）. The level of IL-1β in ThESCs cells was detected by Western blot， and the migration rate and invasion rate of ThESCs cells were detected by Transwell method.

Results

2

The expression of lncRNA-339 was increased in EMs endometrium （

P

<

0.05）. E

2

or overexpression of lncRNA-339 both promoted the expression of lncRNA-339 and IL-1β in ThESCs cells （

P

<

0.05）， and promoted cell migration and invasion （

P

<

0.05）. When E

2

was inhibited， lncRNA-339 was silenced， and IL-1β was inhibited， the cell migration rate and invasion rate were decreased （

P

<

0.05）， and the expression of IL-1β was significantly decreased （

P

<

0.05）.

Conclusion

2

E

2

stimulates the expression of lncRNA-339 to increase， and promotes the migration and invasion of EMs stromal cells by up-regulating IL-1β.

关键词

长链非编码RNA-339白细胞介素-1β子宫内膜异位症子宫内膜基质细胞侵袭迁移

Keywords

long non-coding RNA-339interleukin-1βendometriosisendometrial stromal cellsinvasionmigration

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