Effect of exogenous direct current electric fields inducing angiogenesis in dilated skin soft tissue of rats

LIU Tian; ZHANG Jing-hong; LIU Jie; ZHANG Ze; WU Chao; ZHANG Jia-ping

doi:10.11659/jjssx.04E023024

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Effect of exogenous direct current electric fields inducing angiogenesis in dilated skin soft tissue of rats

Basic Research | 更新时间：2023-09-26

- Effect of exogenous direct current electric fields inducing angiogenesis in dilated skin soft tissue of rats
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 9, Pages: 747-752(2023)
- 作者机构：
  
  1.陆军军医大学第一附属医院整形外科，重庆400038
  2.重庆医科大学附属第二医院整形美容与颌面外科，重庆 400010
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.04E023024
  CLC： R654.3
- Published：25 September 2023，
  
  Received：07 April 2023，
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刘天,张警泓,刘杰等.外源性直流电场诱导大鼠扩张皮肤软组织血管新生的作用研究[J].局解手术学杂志,2023,32(09):747-752.

LIU Tian,ZHANG Jing-hong,LIU Jie,et al.Effect of exogenous direct current electric fields inducing angiogenesis in dilated skin soft tissue of rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(09):747-752.
刘天,张警泓,刘杰等.外源性直流电场诱导大鼠扩张皮肤软组织血管新生的作用研究[J].局解手术学杂志,2023,32(09):747-752. DOI： 10.11659/jjssx.04E023024.

LIU Tian,ZHANG Jing-hong,LIU Jie,et al.Effect of exogenous direct current electric fields inducing angiogenesis in dilated skin soft tissue of rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(09):747-752. DOI： 10.11659/jjssx.04E023024.

摘要

目的

探索外源性直流电场对大鼠扩张皮肤软组织血管新生的诱导作用与机制。

方法

采用SD大鼠作为实验对象，在头皮下埋置微型皮肤扩张器，建立头皮软组织扩张模型。在大鼠扩张头皮头尾两端贴附电极片并施加外源性直流电场刺激(并分为100 mV/mm、200 mV/mm和300 mV/mm电场组)，未刺激的大鼠作为对照组。观察大鼠病理学、组织学和血管生成相关分子表达等变化。

结果

100 mV/mm和200 mV/mm电场组大鼠表现出良好的电场耐受性，300 mV/mm组大鼠出现不同程度的间断性抽搐、焦虑、烦躁等不适宜继续实验的症状表现，终止实验。与对照组相比，100 mV/mm和200 mV/mm电场组大鼠扩张头皮组织角质层厚度显著增大（

0.05），真皮层内及真皮下层微小血管数均显著增加（

0.05)，血管生成相关分子Ki67和FGF-2的表达明显上升（

0.05），组织匀浆中SDF-1和VEGF-α的表达显著升高（

0.05）。

结论

一定强度的外源性直流电场可以有效诱导大鼠扩张皮肤软组织血管新生，为临床上采用电场技术原位诱导血管新生、增加皮瓣切取面积和提高皮瓣存活率提供了实验依据。

Abstract

Objective

To explore the induction effect and mechanism of exogenous direct current electric fields on angiogenesis of dilated skin soft tissue in rats.

Methods

The SD rats were used as experimental subjects， and a micro skin expander was buried under the scalp to establish a dilated scalp skin soft tissue model. The electrode sheets were attached to the head and tail of the dilated scalp and the exogenous direct current electric fields stimulation was conducted （divided into the 100 mV/mm， 200 mV/mm and 300 mV/mm electric field groups）， and the unstimulated rats were used as the control group. The changes of rats in pathology， histology and expression of angiogenesis-related molecules were observed.

Results

The rats in the 100 mV/mm and 200 mV/mm electric field groups showed good electric field tolerances， while rats in the 300 mV/mm group had different degrees of symptoms unsuitable for continuing the experiments including intermittent convulsions， anxiety， irritability，etc.，and their following experiments were terminated. Compared with the control group， the thickness of the stratum corneum of the dilated scalp skin soft tissues of rats in the 100 mV/mm and 200 mV/mm electric field groups increased significantly （

0.05）， the numbers of tiny blood vessels in the dermis and subdermis layer increased significantly （

0.05）， the expression of angiogenesis-related molecules including Ki67 and FGF-2 increased significantly （

0.05）， and the expression of SDF-1 and VEGF-α in tissue homogenate increased significantly （

0.05）.

Conclusion

A certain intensity of exogenous direct current electric fields can effectively induce the angiogenesis of the dilated skin soft tissues in rats， which provides an experimental basis for the clinical use of electric field technology on inducing the angiogenesis in situ， increasing the incision area of flap and improving the survival rate of flap.

关键词

直流电场扩张皮瓣皮瓣移植血管新生血管密度

Keywords

direct current electric fielddilated flapflap transplantationangiogenesisblood vessel density

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