Effect mechanism of dihydromyricetin on the tumor biological characteristics of endometrial cancer cells

CUI Wei-hua; SONG Jing-ying; QIAO Shu-xin; DUAN Shu-zhong

doi:10.11659/jjssx.03E024080

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Effect mechanism of dihydromyricetin on the tumor biological characteristics of endometrial cancer cells

Basic Research | 更新时间：2024-12-10

- Effect mechanism of dihydromyricetin on the tumor biological characteristics of endometrial cancer cells
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 33, Issue 12, Pages: 1039-1043(2024)
- 作者机构：
  
  1.承德医学院第二临床学院/承德市中心医院药学部，河北承德 067000
  2.承德医学院第二临床学院/承德市中心医院内分泌科，河北承德 067000
  3.承德医学院附属医院肾内科，河北承德 067000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.03E024080
  CLC： R915;R737.33
- Published：25 December 2024，
  
  Received：15 March 2024，
- 稿件说明：
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崔伟华,宋静莹,乔淑新等.二氢杨梅素对子宫内膜癌细胞肿瘤生物学特性的影响机制研究[J].局解手术学杂志,2024,33(12):1039-1043.

CUI Wei-hua,SONG Jing-ying,QIAO Shu-xin,et al.Effect mechanism of dihydromyricetin on the tumor biological characteristics of endometrial cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(12):1039-1043.
崔伟华,宋静莹,乔淑新等.二氢杨梅素对子宫内膜癌细胞肿瘤生物学特性的影响机制研究[J].局解手术学杂志,2024,33(12):1039-1043. DOI： 10.11659/jjssx.03E024080.

CUI Wei-hua,SONG Jing-ying,QIAO Shu-xin,et al.Effect mechanism of dihydromyricetin on the tumor biological characteristics of endometrial cancer cells[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(12):1039-1043. DOI： 10.11659/jjssx.03E024080.

摘要

目的

探讨二氢杨梅素对子宫内膜癌（EC）细胞增殖、凋亡及侵袭的影响，并分析其可能的作用机制。

方法

取对数生长期Ishikawa细胞，分为对照组、20 µmol/L二氢杨梅素干预组、40 µmol/L二氢杨梅素干预组、80 µmol/L二氢杨梅素干预组，分别用不同终浓度的二氢杨梅素（0 µmol/L、20 µmol/L、40 µmol/L、80 µmol/L）处理Ishikawa细胞。CCK-8法和流式细胞术检测二氢杨梅素对细胞增殖和凋亡的影响。Transwell实验检测二氢杨梅素对细胞迁移、侵袭的影响。qRT-PCR和Western blot检测二氢杨梅素对细胞miR-21和PTEN表达的影响。

结果

与对照组比较，二氢杨梅素干预组细胞增殖抑制率和凋亡率明显升高（

0.05），且随着二氢杨梅素浓度的增加而逐渐升高（

0.05）；与对照组比较，二氢杨梅素干预组迁移及侵袭细胞数明显减少（

0.05），且随着二氢杨梅素浓度的增加而逐渐减少（

0.05）；与对照组比较，二氢杨梅素干预组细胞miR-21表达明显降低（

0.05），且随着二氢杨梅素浓度的增加而逐渐降低（

0.05），PTEN mRNA和蛋白的表达水平明显增高（

0.05），且随着二氢杨梅素浓度的增加而逐渐升高（

0.05）。

结论

二氢杨梅素能够抑制EC细胞的生长及转移，其抑制作用与浓度呈正相关，其机制可能与二氢杨梅素对EC细胞miR-21/PTEN信号通路的影响有关。

Abstract

Objective

To explore the effects of dihydromyricetin on the proliferation，apoptosis，and invasion of endometrial cancer （EC） cells and its possible mechanisms.

Methods

Ishikawa cells in the logarithmic growth phase were taken and divided into the control group，20 µmol/L dihydromyricetin intervention group，40 µmol/L dihydromyricetin intervention group，and 80 µmol/L dihydromyricetin intervention group，which were treated with different final concentrations of dihydromyricetin （0 µmol/L，20 µmol/L，40 µmol/L，80 µmol/L）. Then，CCK-8 assay and flow cytometry were used to detect the effects of dihydromyricetin on the cell proliferation and apoptosis. Transwell experiment was used to detect the effect of dihydromyricetin on the cell invasion. qRT-PCR and Western blot were used to detect the effects of dihydromyricetin on the cell expression of miR-21 and PTEN.

Results

Compared with the control group，the cell proliferation inhibition rate and apoptosis rate in the dihydromyricetin intervention group were significantly increased （

0.05），and gradually increased with the increase of dihydromyricetin concentration （

0.05）. Compared with the control group，the numbers of migration and invasion cells in the dihydromyricetin intervention group were significantly decreased （

0.05），and gradually decreased with the increase of dihydromyricetin concentration （

0.05）. Compared with the control group，the cell expression of miR-21 in the dihydromyricetin intervention group was significantly decreased （

0.05），and gradually decreased with the increase of dihydrom

yricetin concentration （

0.05），the expression levels of PTEN mRNA and protein were significantly increased （

0.05），and gradually increased with the increase of dihydromyricetin concentration （

0.05）.

Conclusion

Dihydromyricetin can inhibit the growth and metastasis of EC cells，and the inhibitory effect is positively correlated with its concentration. The mechanism may be related to the effect of dihydromyricetin on the miR-21/PTEN signaling pathway of EC cells.

关键词

二氢杨梅素子宫内膜癌细胞增殖细胞凋亡细胞侵袭miR-21PTEN

Keywords

dihydromyricetinendometrial cancercell proliferationcell apoptosiscell invasionmiR-21PTEN

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