Effect of TSP-1 on wound healing of skin combined radiation-wound injury in mice

NAN Sha; LIU Sui-yi; RAN Yong-hong; ZHAO Ya-zhen; HAO Yu-hui

doi:10.11659/jjssx.03E023173

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Effect of TSP-1 on wound healing of skin combined radiation-wound injury in mice

Basic Research | 更新时间：2023-10-23

- Effect of TSP-1 on wound healing of skin combined radiation-wound injury in mice
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 10, Pages: 865-869(2023)
- 作者机构：
  
  陆军军医大学军事预防医学系复合伤研究所，重庆 400038
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.03E023173
  CLC： R146
- Published：25 October 2023，
  
  Received：28 March 2023，
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南莎,柳随义,冉永红等.TSP-1对小鼠皮肤放创复合伤伤口愈合的影响[J].局解手术学杂志,2023,32(10):865-869.

NAN Sha,LIU Sui-yi,RAN Yong-hong,et al.Effect of TSP-1 on wound healing of skin combined radiation-wound injury in mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):865-869.
南莎,柳随义,冉永红等.TSP-1对小鼠皮肤放创复合伤伤口愈合的影响[J].局解手术学杂志,2023,32(10):865-869. DOI： 10.11659/jjssx.03E023173.

NAN Sha,LIU Sui-yi,RAN Yong-hong,et al.Effect of TSP-1 on wound healing of skin combined radiation-wound injury in mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):865-869. DOI： 10.11659/jjssx.03E023173.

摘要

目的

探讨TSP-1在小鼠皮肤放创复合伤（CRWI）伤口愈合过程中的作用。

方法

以TSP-1敲除（KO）小鼠为主要研究对象，以野生型（WT）小鼠作为对照，每组15只，比较WT小鼠与TSP-1KO小鼠皮肤CRWI的伤口愈合情况。取WT小鼠与TSP-1KO小鼠皮肤CRWI建模后第7天的伤口组织进行免疫组化染色以及免疫荧光染色，Western blot分析NF-кB蛋白表达水平，并使用RT-qPCR检测伤口部位炎症细胞因子IL-1β、IL-6和TNF-α的表达水平，以确定2组小鼠皮肤CRWI伤口部位的炎症反应状态及血管生成情况。

结果

与WT小鼠相比，从CRWI建模第3天开始TSP-1KO小鼠皮肤CRWI伤口愈合显著延迟（

0.05）。CRWI建模后第7天TSP-1KO小鼠伤口部位的F4/80巨噬细胞浸润减少（

0.05），NF-кB蛋白表达水平降低（

0.01），炎症细胞因子IL-1β和IL-6的表达水平也相对降低（

0.01），并且伤口部位CD31阳性细胞以及α-SMA阳性细胞数量减少（

0.05）。

结论

敲除TSP-1会导致小鼠皮肤CRWI炎症反应减弱，血管生成受到抑制，伤口愈合延迟。

Abstract

Objective

To investigate the effect of TSP-1 on wound healing process of skin combined radiation-wound injury （CRWI） in mice.

Methods

TSP-1 knockout （KO） mice were used as the main study subjects， and wild-type （WT） mice were used as the control， with 15 mice in each group. The wound healing of skin CRWI between WT mice with TSP-1KO mice was compared. The wound tissues of WT mice and TSP-1KO mice on the 7th day after skin CRWI modeling were taken for immunohistochemical staining and immunofluorescence staining， the NF-кB protein expression levels were analyzed by Western blot， and the expression levels of inflammatory cytokines IL-1β，IL-6 and TNF-α in the wound site were detected by RT-qPCR， so as to determine the inflammatory response and angiogenesis in the skin CRWI wound site of mice in the two groups.

Results

Compared with WT mice， skin CRWI wound healing was significantly delayed in TSP-1KO mice from the 3th day of CRWI modeling （

0.05）. On the 7th day after CRWI modeling， the infiltration of F4/80 macrophages in the wound site of TSP-1KO mice decreased （

0.05）， the NF-кB protein expression level decreased （

0.01）， the inflammatory cytokines IL-1β and IL-6 expression levels were also relatively reduced （

0.01）， and the number of CD31-positive cells as well as α-SMA-positive cells in the wound site were decreased （

0.05）.

Conclusion

Knockout of TSP-1 can weaken the inflammatory response， inhibit angiogenesis and delay wound healing of skin CRWI in mice.

关键词

TSP-1放创复合伤伤口愈合炎症反应血管生成

Keywords

TSP-1combined radiation-wound injurywound healinginflammatory responseangiogenesis

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