Hepatic lipidomics study in chronic cadmium-exposed mice
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 33, Issue 3, Pages: 194-199(2024)
- 作者机构：
陆军军医大学军事预防医学系军队劳动卫生学教研室/教育部电磁辐射医学防护重点实验室，重庆 400038
- 作者简介：
- 基金信息：
DOI：10.11659/jjssx.03E023098
CLC： R995Published：25 March 2024，
Received：15 March 2023，
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郝蓉蓉,李玲,田丽等.慢性镉暴露小鼠的肝脂质组学研究[J].局解手术学杂志,2024,33(03):194-199.
HAO Rong-rong,LI Ling,TIAN Li,et al.Hepatic lipidomics study in chronic cadmium-exposed mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(03):194-199.
郝蓉蓉,李玲,田丽等.慢性镉暴露小鼠的肝脂质组学研究[J].局解手术学杂志,2024,33(03):194-199. DOI： 10.11659/jjssx.03E023098.
HAO Rong-rong,LI Ling,TIAN Li,et al.Hepatic lipidomics study in chronic cadmium-exposed mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(03):194-199. DOI： 10.11659/jjssx.03E023098.
摘要
目的
2
研究慢性镉暴露小鼠肝脂质组学的改变，并筛选出具有显著差异的脂质亚类、脂质分子和富集的代谢通路。
方法
2
选用12只SPF级雄性C57BL/6J小鼠（8周龄），随机分成对照组（正常水喂养）和实验组[镉水（0.6 mg /L CdCl
2
）喂养]，每组6只。在镉暴露6个月后处死小鼠，即刻摘取新鲜的肝组织。对肝组织行脂质油红O染色和脂质组学分析。
结果
2
与对照组相比，实验组小鼠肝组织脂质油红O染色未出现红色。通过脂质组学筛选到17种具有显著差异的脂质亚类，144个具有显著差异的脂质分子。将具有显著差异的脂质分子通过KEGG富集得到以下通路：甘油磷脂代谢、亚油酸代谢、α-亚麻酸代谢、糖基磷脂酰肌醇生物合成、甘油脂代谢、花生四烯酸代谢。
结论
2
本研究通过建立慢性镉暴露模型，揭示了慢性镉暴露会诱导小鼠肝中脂质亚类和脂质代谢物的紊乱，为了解慢性镉暴露导致的非酒精性脂肪性肝病提供依据。
Abstract
Objective
2
To study the change of lipidomics in chronic cadmium-exposed mice，thereby screening out lipid subclasses，lipid molecules and enriched metabolic pathways with significant differences.
Methods
2
Twelve SPF male C57BL/6J mice （8 weeks old） were randomly divided into the control group （normal water feeding） and the experimental group ［cadmium water （0.6 mg/L of CdCl
2
） feeding］，with 6 mice in each group.Mice were sacrificed after 6 months of cadmium exposure，and fresh liver tissues were collected immediately.Lipid oil red O staining and lipidomics analysis were performed on liver tissue.
Results
2
Compared with the control group，the liver tissue of mice in the experimental group did not appear red after lipid oil red O staining.Seventeen lipid subclasses with significant differences and 144 lipid molecules with significant differences were screened out by lipidomics.These lipid molecules with significant differences were enriched in glycerophospholipid metabolism，linoleic acid metabolism，alpha-linolenic acid metabolism，glycosylphosphatidylinositol biosynthesis，glycerolipid metabolism and arachidonic acid metabolism by KEGG.
Conclusion
2
This study reveals that chronic cadmium exposure can induce the disorder of lipid subclasses and lipid metabolites in the liver of mice，which provides a basis for understanding the non-alcoholic fatty liver disease caused by chronic cadmium exposure.
关键词
慢性镉暴露肝脂质组学非酒精性脂肪性肝病
Keywords
chronic cadmium exposureliverlipidomicsnon-alcoholic fatty liver disease
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