Experimental study on esophagojejunal magnamosis in rats

TIAN Bo-yan; ZHANG Miao-miao; WAN Jia-hui; LYU Yi; YAN Xiao-peng

doi:10.11659/jjssx.02E023076

您当前的位置：

首页 >

文章列表页 >

Experimental study on esophagojejunal magnamosis in rats

Basic Research | 更新时间：2023-07-31

- Experimental study on esophagojejunal magnamosis in rats
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 32, Issue 8, Pages: 653-656(2023)
- 作者机构：
  
  1.西安交通大学第一附属医院肝胆外科，陕西西安 710061
  2.西安交通大学第一附属医院精准外科与再生医学国家地方联合工程研究中心，陕西西安 710061
  3.西安医学院临床医学院，陕西西安 710021
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.02E023076
  CLC： R656
- Published：25 August 2023，
  
  Received：15 February 2023，
扫描看全文
田波彦,张苗苗,万嘉辉等.大鼠食管空肠磁吻合实验研究[J].局解手术学杂志,2023,32(08):653-656.

TIAN Bo-yan,ZHANG Miao-miao,WAN Jia-hui,et al.Experimental study on esophagojejunal magnamosis in rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(08):653-656.
田波彦,张苗苗,万嘉辉等.大鼠食管空肠磁吻合实验研究[J].局解手术学杂志,2023,32(08):653-656. DOI： 10.11659/jjssx.02E023076.

TIAN Bo-yan,ZHANG Miao-miao,WAN Jia-hui,et al.Experimental study on esophagojejunal magnamosis in rats[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(08):653-656. DOI： 10.11659/jjssx.02E023076.

摘要

目的

以SD大鼠为模型探讨磁吻合技术在食管空肠侧侧吻合重建中的可行性。

方法

10只SD大鼠，麻醉后取上腹部正中切口，将近端空肠提至食管下端附近，经口置入1枚圆形磁体至胃内，棉签推送磁体至近端空肠，此时经口置入另1枚圆形磁体至食管下端，空肠内磁体与食管内磁体自动相吸，建立食管空肠侧侧磁吻合模型。记录吻合操作时间，观察大鼠术后进食情况，记录磁体排出时间。1个月后处死动物，获取食管空肠侧侧吻合口标本，肉眼及组织学观察吻合口形成情况。

结果

10只SD大鼠均成功完成了食管空肠侧侧吻合，整个手术操作过程顺利，术中未出现消化道损伤、出血等并发症，吻合操作时间(4.65±0.75)min；术后大鼠正常进食，无消化道梗阻表现；术后磁体排出时间为(11.30±1.77) d。所有大鼠术后1个月存活率为100%。术后1个月获取吻合口标本，肉眼观察可见吻合口形成良好，HE和Masson染色可见吻合口黏膜光滑平整，吻合口各层组织连续性良好。

结论

利用磁吻合技术可在食管与空肠之间建立良好的侧侧吻合，是对食管空肠磁吻合研究的有益探索。

Abstract

Objective

To investigate the feasibility of magnamosis technology in the reconstruction of side-to-side esophagojejunostomy in SD rats.

Methods

After anesthesia， the midline incision of the upper abdomen in 10 SD rats were made，and the proximal jejunum was lifted to the lower end of the esophagus. A circular magnet was inserted into the stomach through the mouth and pushed into the proximal jejunum by a cotton swab；at this time， another circular magnet was inserted into the lower end of the esophagus through the mouth， and the magnet in the jejunum and the magnet in the esophagus were automatically attracted， the model of esophagojejunal side-to-side magnamosis was established. The operation time of anastomosis was recorded， the postoperative feeding status of rats was observed， and the magnet discharge time was recorded. The animals were killed 1 month later and the side-to-side esophagojejunal anastomosis specimens were obtained， and the formation of anastomotic stoma was observed by naked eye and histology.

Results

The side-to-side esophagojejunostomy in all 10 SD rats were successfully completed， the whole operation process was smooth， and no complications such as gastrointestinal injury or bleeding during the operation occurred. The operation time of anastomosis was （4.65±0.75） minutes. After the operation， the feeding status of rats were normal without gastrointestinal obstruction. The postoperative magnet discharge time was （11.30±1.77） days. The 1-month survival rate of all rats after operation was 100%. The anastomotic specimens were obtained 1 month after the operation. The anastomotic stoma was well formed by naked eye observation. HE and Masson staining showed that the mucosa of anastomotic stoma was smooth， and the tissue continuity of each layer of the anastomotic stoma was good.

Conclusion

Magnamosis technology can be used to establish a good side-to-side anastomosis between the esophagus and jejunum， which is a beneficial exploration in the research of esophagojejunal magnamosis.

关键词

磁吻合磁外科磁压榨技术食管空肠吻合大鼠

Keywords

magnamosismagnetosurgerymagnetic compression techniqueesophagojejunostomyrats

references

Sung H， Ferlay J， Siegel RL， et al. Global cancer statistics 2020： GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］. CA Cancer J Clin， 2021，71（3）：209-249. doi： 10.3322/caac.21660http://dx.doi.org/10.3322/caac.21660.

余佩武，赵永亮. 全腹腔镜全胃切除术消化道重建方式选择及技术要点［J］. 中国实用外科杂志，2016，36（9）：942-945. doi： 10.7504/CJPS.ISSN1005-2208.2016.09.05http://dx.doi.org/10.7504/CJPS.ISSN1005-2208.2016.09.05.

Edholm D. Systematic review and meta-analysis of circular- and linear-stapled gastro-jejunostomy in laparoscopic Roux-en-Y gastric bypass［J］. Obes Surg， 2019，29（6）：1946-1953. doi： 10.1007/s11695-019-03803-whttp://dx.doi.org/10.1007/s11695-019-03803-w.

苗儒林，李子禹，王胤奎，等. 腹腔镜全胃切除术消化道重建吻合器选择回顾性对照研究［J］. 中国实用外科杂志，2016，36（9）：968-972. doi： 10.7504/CJPS.ISSN1005-2208.2016.09.13http://dx.doi.org/10.7504/CJPS.ISSN1005-2208.2016.09.13.

张苗苗，吉琳，牟星宜，等. 磁吻合研究现状与发展趋势［J］. 中国医疗设备，2020，35（11）：45-48. doi：11.3969/j.issn.1674-1633.2020.11.013http://dx.doi.org/11.3969/j.issn.1674-1633.2020.11.013.

严小鹏，刘雯雁，李涤尘，等. 消化外科手术的内镜化途径：磁吻合联合内镜［J］. 世界华人消化杂志，2014，22（19）：2716-2721.

张苗苗，吉琳，刘培楠，等. 磁压榨技术用于食管吻合重建的实验研究［J］. 中国胸心血管外科临床杂志，2022，29（1）：95-99. doi：10.7507/1007-4848.202103066http://dx.doi.org/10.7507/1007-4848.202103066.

叶丹，陈雯雯，高慧敏，等. 食管胃磁吻合动物实验研究［J］. 中国医疗设备，2020，35（11）：52-54. doi：11.3969/j.issn.1674-1633.2020.11.015http://dx.doi.org/11.3969/j.issn.1674-1633.2020.11.015.

吝怡，樊茜，朱森林，等. 磁吻合环建立兔小肠侧侧吻合的实验研究［J］. 医疗卫生装备，2020，41（4）：18-21. doi：10.19745/j.1003-8868.2020079http://dx.doi.org/10.19745/j.1003-8868.2020079.

Bai J， Huo X， Ma J， et al. Magnetic compression technique for colonic anastomosis in rats［J］. J Surg Res， 2018，231：24-29. doi： 10.1016/j.jss.2018.05.006http://dx.doi.org/10.1016/j.jss.2018.05.006.

吉琳，常凯曦，王俊翔，等. 磁环构建大鼠胆肠吻合模型的实验研究［J］. 中国医疗设备，2020，35（11）：49-51. doi：11.3969/j.issn.1674-1633.2020.11.014http://dx.doi.org/11.3969/j.issn.1674-1633.2020.11.014.

Wang HH， Ma J， Wang SP， et al. Magnetic anastomosis rings to create portacaval shunt in a canine model of portal hypertension［J］. J Gastrointest Surg， 2019，23（11）：2184-2192. doi： 10.1007/s11605-018-3888-5http://dx.doi.org/10.1007/s11605-018-3888-5.

Zhang M， Ma J， An Y， et al. Construction of an intrahepatic portosystemic shunt using the magnetic compression technique： preliminary experiments in a canine model［J］. Hepatobiliary Surg Nutr， 2022，11（4）：611-615. doi： 10.21037/hbsn-22-209http://dx.doi.org/10.21037/hbsn-22-209.

邓博，徐庶钦，王方舟，等. 磁压榨技术治疗输尿管远端梗阻的实验研究［J］. 中国临床解剖学杂志，2021，39（3）：319-322. doi：10.13418/j.issn.1001-165x.2021.03.013http://dx.doi.org/10.13418/j.issn.1001-165x.2021.03.013.

张苗苗，冒健骐，任宇翔，等. 基于磁压榨技术的膀胱造瘘动物实验研究［J］. 中国医疗设备，2022，37（6）：12-14. doi：10.3969/j.issn.1674-1633.2022.06.004http://dx.doi.org/10.3969/j.issn.1674-1633.2022.06.004.

张苗苗，吉琳，盖敬慈，等. 基于磁压榨技术的无创化胃造瘘的大鼠模型研究［J］. 中国普通外科杂志，2021，30（10）：1175-1183. doi：10.7659/j.issn.1005-6947.2021.10.007http://dx.doi.org/10.7659/j.issn.1005-6947.2021.10.007.

高慧敏，邱明龙，张砚超，等. 磁压榨超微创技术建立兔获得性气管食管瘘动物模型［J］.中国临床解剖学杂志，2019，37（2）：223-227. doi：10.13418/j.issn.1001-165x.2019.02.022http://dx.doi.org/10.13418/j.issn.1001-165x.2019.02.022.

叶丹，邱明龙，高慧敏，等. 磁压榨技术治疗小儿食管闭锁和狭窄的临床应用探索［J］. 中华小儿外科杂志，2020，41（4）：370-374. doi： 10.3760/cma.j.cn421158-20190110-00011http://dx.doi.org/10.3760/cma.j.cn421158-20190110-00011.

Zhang M， Lyu X， Zhao G， et al. Establishment of Yan-Zhang's staging of digestive tract magnetic compression anastomosis in a rat model［J］. Sci Rep， 2022，12（1）：12445. doi： 10.1038/s41598-022-16794-zhttp://dx.doi.org/10.1038/s41598-022-16794-z.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Animal experimental study of single-channel transanal magnetic anastomosis ring in the treatment of rectal stenosis

Chrysin attenuating renal tissue damage in rats with diabetes mellitus by inhibiting IRE1α/TXNIP/NLRP3 pathway

Establishment skills of a rat model of abdominal heterotopic heart transplantation by single operator

Design and experimental study of magnetic control device for the treatment of female stress urinary incontinence

Related Author

SHI Ai-hua

ZHAO Lin

GONG Ming-yan

WANG Juan-juan

LI Chen-guang

TIAN Xiao-hui

ZHANG Tao

ZHANG Wen-tao

Related Institution

Department of Critical Care Medicine， China-Japan Union Hospital of Jilin University

Qide College， Xi´an Jiaotong University， Xi´an Shaanxi

Zonglian College， Xi´an Jiaotong University， Xi´an Shaanxi

Department of Hematology and Endocrinology，Xianyang Hospital of Yan'an University

Department of Nephrology，Xianyang Hospital of Yan'an University

⁰