hsa_circ_0011946 regulates the proliferation, migration and invasion of cervical cancer cells via targeting miR-767-3p

LI Wen-ya; XIE Cheng; PENG Li

doi:10.11659/jjssx.02E023047

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hsa_circ_0011946 regulates the proliferation, migration and invasion of cervical cancer cells via targeting miR-767-3p

Basic Research | 更新时间：2024-02-02

- hsa_circ_0011946 regulates the proliferation, migration and invasion of cervical cancer cells via targeting miR-767-3p
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 33, Issue 2, Pages: 118-124(2024)
- 作者机构：
  
  1.长江大学附属仙桃市第一人民医院妇产科，湖北仙桃 433000
  2.长江大学附属仙桃市第一人民医院肾内科，湖北仙桃 433000
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.02E023047
  CLC： R737.33
- Published：25 February 2024，
  
  Received：10 February 2023，
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李文亚,谢程,彭莉.hsa_circ_0011946靶向miR-767-3p调控宫颈癌细胞增殖、迁移和侵袭[J].局解手术学杂志,2024,33(02):118-124.

LI Wen-ya,XIE Cheng,PENG Li.hsa_circ_0011946 regulates the proliferation, migration and invasion of cervical cancer cells via targeting miR-767-3p[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(02):118-124.
李文亚,谢程,彭莉.hsa_circ_0011946靶向miR-767-3p调控宫颈癌细胞增殖、迁移和侵袭[J].局解手术学杂志,2024,33(02):118-124. DOI： 10.11659/jjssx.02E023047.

LI Wen-ya,XIE Cheng,PENG Li.hsa_circ_0011946 regulates the proliferation, migration and invasion of cervical cancer cells via targeting miR-767-3p[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(02):118-124. DOI： 10.11659/jjssx.02E023047.

摘要

目的

探讨hsa_circ_0011946靶向miR-767-3p在宫颈癌进展中的作用。

方法

RT-qPCR分析hsa_circ_0011946、miR-767-3p在宫颈癌组织、癌旁正常组织、人宫颈上皮永生化细胞（H8）及宫颈癌细胞（SiHa、HeLa、Caski）中的表达水平。分别用si-NC、si-hsa_circ_0011946、miR-NC、miR-767-3p、pcDNA、pcDNA-hsa_circ_0011946、anti-miR-NC+si-hsa_circ_0011946、anti-miR-767-3p+si-hsa_circ_0011946转染SiHa细胞，并设为si-NC组、si-hsa_circ_0011946组、miR-NC组、miR-767-3p组、pcDNA组、pcDNA-hsa_circ_0011946组、anti-miR-NC+si-hsa_circ_0011946组、anti-miR-767-3p+si-hsa_circ_0011946组，未转染的细胞作为NC组。采用RT-qPCR检测各组SiHa细胞中hsa_circ_0011946和miR-767-3p的表达；CCK-8法检测细胞活力；平板克隆形成实验检测细胞克隆数；Transwell实验检测细胞迁移和侵袭数；Western blot检测MMP-2、MMP-9蛋白表达。circular RNA interactome预测miR-767-3p与hsa_circ_0011946的结合位点，双荧光素酶报告基因实验分析hsa_circ_0011946和miR-767-3p的靶向关系。

结果

与癌旁正常组织比较，宫颈癌组织中hsa_circ_0011946表达水平显著升高（

0.05），miR-767-3p表达水平显著降低（

0.05）。与H8细胞比较，SiHa、HeLa、Caski细胞中hsa_circ_0011946表达水平显著升高（

0.05），miR-767-3p表达水平显著降低（

0.05）。与NC组比较，si-hsa_circ_0011946组SiHa细胞hsa_circ_0011946表达水平、吸光度（A）值、克隆数、迁移数、侵袭数及MMP-2、MMP-9蛋白表达水平显著降低/减少（

0.05）。与miR-NC组比较，miR-767-3p组SiHa细胞miR-767-3p表达水平显著升高（

0.05），A值、克隆数、迁移数、侵袭数及MMP-2、MMP-2蛋白表达水平显著降低/减少（

0.05）。与pcDNA组比较，pcDNA-hsa_circ_0011946组SiHa细胞miR-767-3p表达水平显著降低（

0.05），hsa_circ_0011946表达水平显著升高（

0.05）。共转染miR-767-3p mimics和WT-hsa_circ_0011946的SiHa细胞相对荧光素酶活性低于共转染miR-NC和WT-hsa_circ_0011946（

0.05）。hsa_circ_0011946与miR-767-3p直接特异性结合。与anti-miR-NC+si-hsa_circ_0011946组比较，anti-miR-767-3p+si-hsa_circ_0011946组SiHa细胞miR-767-3p表达水平显著降低（

0.05），A值、克隆数、迁移数、侵袭数及MMP-2、MMP-9蛋白表达水平显著升高/增加（

0.05）。

结论

干扰hsa_circ_0011946通过靶向上调miR-767-3p抑制宫颈癌细胞的增殖、迁移和侵袭。

Abstract

Objective

To investigate the role of hsa_circ_0011946 targeting miR-767-3p in the progression of cervical cancer.

Methods

The expression levels of hsa_circ_0011946 and miR-767-3p in cervical cancer tissues， adjacent tissues， immortalized human cervical epithelial cells （H8） and cervical cancer cells （SiHa， HeLa， and Cashi） were determined by RT-qPCR. SiHa cells were transfected with si-NC， si-hsa_circ_0011946， miR-NC， miR-767-3p， pcDNA， pcDNA-hsa_circ_0011946， anti-miR-NC+si-hsa_circ_0011946， anti-miR-767-3p+si-hsa_circ_0011946， respectively， as the si-NC group， the si-hsa_circ_0011946 group， the miR-NC group， the miR-767-3p group， the pcDNA group， the pcDNA-hsa_circ_0011946 group， the anti-miR-NC+si-hsa_circ_0011946 group， and the anti-miR-767-3p+si-hsa_circ_0011946 group， and the untransfected cells were used as the NC group. The expression of hsa_circ_0011946 and miR-767-3p of SiHa cells in each group was detected by RT-qPCR， the cell viability was detected by CCK-8， the number of cell clone was detected by plate clone formation assay， the cell migration and invasion numbers were detected by Transwell assay， and the protein expression of MMP-2 and MMP-9 was detected by Western blot. The binding site of miR-767-3p to hsa_circ_0011946 was predicted by circular RNA interactome， and the targeted relationship between hsa_circ_0011946 and miR-767-3p was analyzed by dual luciferase reporter assay.

Results

Compared with the adjacent tissues， the expression level of hsa_circ_0011946 in the cervical cancer tissues was significantly increased （

0.05）， while the expression level of miR-767-3p was significantly decreased （

0.05）. Compared with H8 cells， the expression levels of hsa_circ_0011946 in SiHa， HeLa and Caski cells were significantly increased （

0.05）， while the expression levels of miR-767-3p were significantly decreased （

0.05）. Compared with the NC group， the expression level of hsa_circ_0011946，absorbance （A） value， number of clone， number of migration， number of invasion and the protein expression levels of MMP-2 and MMP-9 were significantly decreased in SiHa cells in the miR-767-3p group （

0.05）. Compared with the miR-NC group， the expression level of miR-767-3p in SiHa cells in the miR-767-3p group was significantly increased （

0.05）， and the cell A value， number of clone， number of migration， number of invasion and the protein expression levels of MMP-2 and MMP-9 were significantly decreased （

0.05）. Compared with the pcDNA group， the expression level of miR-767-3p in SiHa cells in the pcDNA-hsa_circ_0011946 group was significantly decreased （

0.05）， and the expression level of hsa_circ_0011946 was significantly increased （

0.05）. The relative luciferase activity of SiHa cells co-transfected with miR-767-3p mimics and WT-hsa_circ_0011946 was lower than that of co-transfected with miR-NC and WT-hsa_circ_0011946 （

0.05）. hsa_circ_0011946 bound to miR-767-3p directly and specifically. Compared with the anti-miR-NC+si-hsa_circ_0011946 group， the expression level of miR-767-3p in SiHa cells in the anti-miR-767-3p+si-hsa_circ_0011946 group was significantly decreased （

0.05）， and the cell A value， number of clone， number of migration， number of invasion and protein expression levels of MMP-2 and MMP-9 were significantly increased （

0.05）.

Conclusion

Interfering hsa_circ_0011946 can inhibit the proliferation， migration and invasion of cervical cancer cells through targeted up-regulation of miR-767-3p.

关键词

hsa_circ_0011946miR-767-3p宫颈癌增殖迁移侵袭

Keywords

hsa_circ_0011946miR-767-3pcervical cancerproliferationmigrationinvasion

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