Metabonomic study of blood of mice with high-voltage electrical injury
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 33, Issue 2, Pages: 100-105(2024)
- 作者机构：
陆军军医大学军事预防医学系军队劳动卫生学教研室/教育部电磁辐射医学防护重点实验室，重庆 400038
- 作者简介：
- 基金信息：
DOI：10.11659/jjssx.01E023037
CLC： R135.99Published：25 February 2024，
Received：08 January 2023，
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陈思羽,王徽,罗燕等.高压电击伤小鼠的血液代谢组学研究[J].局解手术学杂志,2024,33(02):100-105.
CHEN Si-yu,WANG Hui,LUO Yan,et al.Metabonomic study of blood of mice with high-voltage electrical injury[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(02):100-105.
陈思羽,王徽,罗燕等.高压电击伤小鼠的血液代谢组学研究[J].局解手术学杂志,2024,33(02):100-105. DOI： 10.11659/jjssx.01E023037.
CHEN Si-yu,WANG Hui,LUO Yan,et al.Metabonomic study of blood of mice with high-voltage electrical injury[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(02):100-105. DOI： 10.11659/jjssx.01E023037.
摘要
目的
2
探讨暴露于高压电击后小鼠的血液代谢组学变化，并筛选出其中显著改变的差异代谢物和代谢通路。
方法
2
将C57BL/6J小鼠头部进行高压电击处理（电击组）和相同电击装置的声光刺激暴露（对照组）后，取全血并分离出血清，进行基于气相色谱-质谱（GC-MS）/液相色谱-反谱（LC-MS）的双平台联用代谢组学分析，采用正交偏最小二乘法（OPLS-DA）模型筛选其差异代谢物和相关代谢通路。
结果
2
高压电击后小鼠的血液代谢组学筛选出415种差异代谢物，包含上调187种，下调228种；这些差异代谢物被显著富集到的代谢通路包括癌症中心碳代谢、胰高血糖素信号通路等。
结论
2
本研究通过建立实验小鼠高压电击伤模型，揭示高压电击伤引起血液中代谢物含量和代谢通路的显著变化，为高压电击伤损害血液代谢活动提供了依据，提示高压电击伤对全身血液代谢活动的潜在危害。
Abstract
Objective
2
To explore the changes of metabonomics in blood of mice after high-voltage electric shock，then screen out the significantly changed differential metabolites and metabolic pathways.
Methods
2
The head of C57BL/6J mice was subjected to high-voltage electric shock （electric shock group） or exposed to acoustic and optical stimulation of high-voltage electric （control group），then the whole blood from mice were collected to separate serum. The dual platform combined metabonomic analysis based on gas chromatography-mass spectrometer （GC-MS） and liquid chromatography-mass spectrometer （LC-MS） was performed and orthogonal partial least squares discriminant analysis（OPLS-DA） was used to screen the differential metabolites and related metabolic pathways.
Results
2
A total of 415 differential metabolites were screened out in metabonomics in blood of mice after high-voltage electric shock，including 187 up-regulated and 228 down-regulated metabolites. These differentially metabolites were significantly enriched in metabolic pathways including central carbon metabolism in cancer，glucagon signaling pathway，etc.
Conclusion
2
By establishing the model of high-voltage electrical injury on experimental mice，this study reveals the significant change of metabolite content and metabolic pathway in blood by high-voltage electrical injury.Which provides a basis for the damage of blood metabolic activity by high-voltage electrical injury，and suggests the potential harm of high-voltage electrical injury to blood metabolic activity in the whole body.
关键词
电工作业高压电击伤血液血生化代谢组学
Keywords
electrician operationhigh-voltage electrical injurybloodblood biochemistrymetabolomics
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