Role of lncRNA RMRP in the proliferation and apoptosis of endometrial carcinoma cells through JAK/STAT signaling pathway

FANG Qiu-man; DENG Qing-chun; ZHOU Xiao-fei; HUANG Cong-mei

doi:10.11659/jjssx.01E023012

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Role of lncRNA RMRP in the proliferation and apoptosis of endometrial carcinoma cells through JAK/STAT signaling pathway

Basic Research | 更新时间：2024-04-01

- Role of lncRNA RMRP in the proliferation and apoptosis of endometrial carcinoma cells through JAK/STAT signaling pathway
- JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY Vol. 33, Issue 3, Pages: 200-207(2024)
- 作者机构：
  
  海南医学院第二附属医院妇科，海南海口 570311
- 作者简介：
- 基金信息：
- DOI：10.11659/jjssx.01E023012
  CLC： R737.33
- Published：25 March 2024，
  
  Received：03 January 2023，
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方秋满,邓青春,周小飞等.lncRNA RMRP通过JAK/STAT信号通路在子宫内膜癌细胞增殖和凋亡中的作用研究[J].局解手术学杂志,2024,33(03):200-207.

FANG Qiu-man,DENG Qing-chun,ZHOU Xiao-fei,et al.Role of lncRNA RMRP in the proliferation and apoptosis of endometrial carcinoma cells through JAK/STAT signaling pathway[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(03):200-207.
方秋满,邓青春,周小飞等.lncRNA RMRP通过JAK/STAT信号通路在子宫内膜癌细胞增殖和凋亡中的作用研究[J].局解手术学杂志,2024,33(03):200-207. DOI： 10.11659/jjssx.01E023012.

FANG Qiu-man,DENG Qing-chun,ZHOU Xiao-fei,et al.Role of lncRNA RMRP in the proliferation and apoptosis of endometrial carcinoma cells through JAK/STAT signaling pathway[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(03):200-207. DOI： 10.11659/jjssx.01E023012.

摘要

目的

探讨长链非编码RNA RMRP（lncRNA RMRP）在子宫内膜癌中的生物学功能及主要分子机制。

方法

收集在我院接受手术治疗的30例子宫内膜癌患者的癌组织和癌旁组织标本，RT-qPCR法检测lncRNA RMRP在子宫内膜癌组织和癌旁组织、HESC细胞和HEC-1-A细胞中的表达。体外培养子宫内膜癌细胞系HEC-1-A，将空载体、pcDNA-RMRP、NC-siRNA、RMRP-siRNA、NC mimic、miR-580-3p mimic、pcDNA-RMRP+NC mimic、pcDNA-RMRP+miR-580-3p mimic、RMRP-siRNA+空载体、RMRP-siRNA+pcDNA-JAK2、NC inhibitor、miR-580-3p inhibitor分别转染至HEC-1-A细胞中，作为空载体组、pcDNA-RMRP组、NC-siRNA组、RMRP-siRNA组、NC mimic组、miR-580-3p mimic组、pcDNA-RMRP+NC mimic组、pcDNA-RMRP+miR-580-3p mimic组、RMRP-siRNA+空载体组、RMRP-siRNA+pcDNA-JAK2组、NC inhibitor组、miR-580-3p inhibitor组。RT-qPCR检测lncRNA RMRP、miR-580-3p在细胞中的表达；CCK-8法检测细胞增殖率；流式细胞术检测细胞凋亡率；生物信息学软件和双荧光素酶报告基因实验分别预测和验证miR-580-3p与lncRNA RMRP、JAK2的靶向互作关系；Western blot检测JAK/STAT信号通路蛋白表达。

结果

与癌旁组织相比，lncRNA RMRP在子宫内膜癌组织中均显著高表达（

0.05）。与HESC细胞比较，lncRNA RMRP在HEC-1-A细胞中的表达显著升高（

0.05）。pcDNA-RMRP可显著促进细胞增殖，抑制细胞凋亡，而RMRP-siRNA可显著抑制细胞增殖，促进细胞凋亡，差异均有统计学意义（

0.05）。miR-580-3p是lncRNA RMRP的下游靶miRNA，lncRNA RMRP可负向调控miR-580-3p的表达。JAK2是miR-580-3p的下游靶基因，miR-580-3p可负向调控JAK2蛋白的表达。pcDNA-RMRP可显著增加细胞中JAK2、p-JAK2和p-STAT3蛋白水平，而pcDNA-RMRP与miR-580-3p mimic共转染后，细胞中JAK2、p-JAK2、p-STAT3蛋白水平显著降低，差异均有统计学意义（

0.05）。RMRP-siRNA可显著降低细胞中JAK2、p-JAK2、p-STAT3蛋白水平，而RMRP-siRNA与pcDNA-JAK2共转染后，细胞中JAK2、p-JAK2、p-STAT3蛋白水平显著升高，差异均有统计学意义（

0.05）。此外，RMRP-siRNA与pcDNA-JAK2共转染后，细胞增殖率升高，细胞凋亡率降低，差异均有统计学意义（

0.05）。

结论

敲低lncRNA RMRP通过调控JAK2/STAT3信号通路抑制子宫内膜癌细胞增殖，促进细胞凋亡，可能成为子宫内膜癌潜在的治疗靶点。

Abstract

Objective

To investigate the biological function and main molecular mechanism of long noncoding RNA RMRP （lncRNA RMRP） in endometrial carcinoma.

Methods

The specimens of carcinoma tissues and adjacent tissues of 30 patients with endometrial carcinoma who received surgical treatment in our hospital were collected.RT-qPCR was used to detect the expression of lncRNA RMRP in endometrial carcinoma tissues and adjacent tissues，and HESC cells and HEC-1-A cells.The endometrial carcinoma cell line HEC-1-A was cultured in vitro，and the Vector，pcDNA-RMRP，NC-siRNA，RMRP-siRNA，NC mimic，miR-580-3p mimic，pcDNA-RMRP+NC mimic，pcDNA-RMRP+miR-580-3p mimic，RMRP-siRNA+Vector，RMRP-siRNA+pcDNA-JAK2，NC inhibitor，and miR-580-3p inhibitor were transfected into HEC-1-A cells as the Vector group，the pcDNA-RMRP group，the NC-siRNA group，the RMRP-siRNA group，the NC mimic group，the miR-580-3p mimic group，the pcDNA-RMRP+NC mimic group，the pcDNA-RMRP+miR-580-3p mimic group，the RMRP siRNA+Vector group，the RMRP-siRNA+pcDNA-JAK2 group，the NC inhibitor group， and the miR-580-3p inhibitor group respectively.RT-qPCR was used to detect the expression of lncRNA RMRP and miR-580-3p in cells.CCK-8 was used to detect cell proliferation rate.The apoptosis rate was detected by flow cytometry analysis.Bioinformatics software and dual luciferase reporter gene experiment were used to predict and verify the targeted relationships between miR-580-3p and lncRNA RMRP，as well as miR-580-3p and JAK2.Western blot was used to detect the protein expression of JAK/STAT signaling pathway.

Results

Compared with adjacent tissues，lncRNA RMRP was highly expressed in endometrial carcinoma tissues （

0.05）. Compared with HESC cells，the expression of lncRNA RMRP in HEC-1-A cells was significantly increased （

0.05）.pcDNA-RMRP significantly promoted cell proliferation and inhibited cell apoptosis，while RMRP-siRNA significantly inhibited cell proliferation and promoted cell apoptosis，with statistically significant diferences （

0.05）.miR-580-3p was the downstream target miRNA of lncRNA RMRP，and lncRNA RMRP could negatively regulate the expression of miR-580-3p.JAK2 was the downstream target gene of miR-580-3p，and miR-580-3p could negatively regulate the expression of JAK2 protein.pcDNA-RMRP significantly increased the protein levels of JAK2，p-JAK2 and p-STAT3 in the cells，while co-transfection of pcDNA-RMRP and miR-580-3p mimic significantly decreased the protein levels of JAK2，p-JAK2 and p-STAT3，with statistically significant diferences （

0.05）.RMRP-siRNA could significantly reduce the protein levels of JAK2，p-JAK2 and p-STAT3 in cells.After co-transfection of RMRP-siRNA and pcDNA-JAK2，the protein levels of JAK2，p-JAK2 and p-STAT3 were significantly increased，with statistically significant diferences （

0.05）.In addition，co-transfection of RMRP-siRNA and pcDNA-JAK2 increased cell proliferation and decreased cell apoptosis，with statistically significant diferences （

0.05）.

Conclusion

Knockdown of lncRNA RMRP could inhibit endometrial carcinoma cell proliferation and promote cell apoptosis by regulating JAK2/STAT3 signaling pathway，which might be a potential therapeutic target for endometrial carcinoma.

关键词

长链非编码RNA RMRP子宫内膜癌增殖凋亡JAK/STAT信号通路

Keywords

long noncoding RNA RMRPendometrial carcinomaproliferationapoptosisJAK/STAT signaling pathway

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