Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing

Xin Qiu; Hao-Yu Wang; Zhen-Yu Yang; Li-Ming Sun; Shu-Nan Liu; Chui-Qin Fan; Feng Zhu

doi:10.1016/j.gendis.2023.01.005

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Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing

Full-length article | 更新时间：2024-02-04

- Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing
- Genes & Diseases Vol. 1, Issue 6, Pages: 2597-2613(2023)
- 作者机构：
  
  1. Department of Spinal Surgery, The University of Hong Kong-Shenzhen Hospital,Guangdong,Shenzhen,China,518000
  2. Department of Orthopedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong,Hong Kong SAR,China,999077
  3. College of Life Sciences, University of Chinese Academy of Sciences,Beijing,China,100000
  4. Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-, Qingdao,Shandong,Qingdao,China,266000
  5. Department of Plastic and Reconstructive Surgery, Xijing Hospital, Forth Military Medical University,Shaanxi,Xi'an,China,710000
  6. China Medical University,Liaoning,Shenyang,China,110000
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2023.01.005
  CLC：
- Published：2023，
  
  Published Online：1 November 2023，
扫描看全文
Xin Qiu, Hao-Yu Wang, Zhen-Yu Yang, et al. Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing. [J]. Genes & Diseases 1(6):2597-2613(2023)
DOI：

Xin Qiu, Hao-Yu Wang, Zhen-Yu Yang, et al. Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing. [J]. Genes & Diseases 1(6):2597-2613(2023) DOI： 10.1016/j.gendis.2023.01.005.

摘要

Abstract

To uncover the role of satellite cells (SCs) in paravertebral muscle development and aging

we constructed a single-nucleus transcriptomic atlas of mouse paravertebral muscle across seven timepoints spanning the embryo (day 16.5) to old (month 24) stages. Eight cell types

including SCs

fast muscle cells

and slow muscle cells

were identified. An energy metabolism-related gene set

TCA CYCLE IN SENESCENCE

was enriched in SCs. Forty-two skeletal muscle disease-related genes were highly expressed in SCs and exhibited similar expression patterns. Among them

Pdha1

was the core gene in the TCA CYCLE IN SENESCENCE;

Pgam2

Sod1

and

Suclg1

are transcription factors closely associated with skeletal muscle energy metabolism. Transcription factor enrichment analysis of the 42 genes revealed that

Myod1

and

Mef2a

were also highly expressed in SCs

which regulated

Pdha1

expression and were associated with skeletal muscle development. These findings hint that energy metabolism may be pivotal in SCs development and aging. Three ligand-receptor pairs of extracellular matrix (ECM)-receptor interactions

Lamc1

Dag1

Lama2

Dag1

and

Hspg2

Dag1

may play a vital role in SCs interactions with slow/fast muscle cells and SCs self-renewal. Finally

we built the first database of a skeletal muscle single-cell transcriptome

the Musculoskeletal Cell Atlas (http://www.mskca.tech)

which lists 630

040 skeletal muscle cells and provides interactive visualization

a useful resource for revealing skeletal muscle cellular heterogeneity during development and aging. Our study could provide new targets and ideas for developing drugs to inhibit skeletal muscle aging and treat skeletal muscle diseases.

关键词

Keywords

NicheParaspinal muscleSatellite cellsSenescenceSingle-nucleus RNA sequencing

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