Objective To discuss the effect of team-based learning(TBL) combined with net-work environment in pathological experiment teaching. Methods Totally 112 clinical medical under-graduates were selected as research object. Digital microscope interactive teaching platform under the network environment was used in experimental group(n=56). In pathology experiment teaching, TBL steps of training, teaching plan demonstrating, grouping, teaching task arranging, asking questions, group learning and communicating were implemented. Traditional method (lecture) was used in con-trol group (n=56). Teaching effect was analyzed through questionnaire survey and score analysis. Stu-dents' evaluation on TBL teaching and network environment resources was expressed as percentage. Results In experiment group, average score was (85.5±3.11) points and average final exam score was (83.8±2.53) points, while in control group, average final exam score was (76.4±11.89) points. There were significance differences between the two groups (t=7.018, P<0.01). Surveys showed that most students accepted TBL teaching combined with network environment. Conclusions TBL teach-ing combined with network environment is feasible and effective in the pathology experimental class. Students' learning enthusiasm is generally improved and effectiveness of TBL teaching is satisfactory.