Objective　To understand the short-term effect of practice skill training for general practitioners in Guangdong province and to provide scientific basis for improving the training strategy. Methods　According to the training outline （Outline of job-transfer training for general practitioner in primary health institutions （trial）） of the Ministry of Health， job-transfer training was provided for a total of 451 primary care doctors in economically undeveloped cities for one year. All trainees were investigated before and after training by questionnaire， which were designed by ourselves and included practical skill level and satisfaction degree.SPSS １３.0 software was used to do statistical analysis and data were processed by description analysis， t test， ANOVA and correlation analysis. Results　Average self-evaluation scores of practical skills were 136.37±21.74 and 169.39±17.12 before and after training with significant differences （t=29.028， P=0.000）. Scores of all types of practical skills after training were significantly higher than those before training （P<0.000 1）. Total satisfaction rate was 91.6% and students were not well satis-fied with training environment and training guarantee. Fac-tors influencing the effect of training included educational background， professional title， doctors' de-sire of training， whether taking part in the general practitioner training or not. The effect of practical training was positively correlated with total satisfaction （r＝0.162， P＝0.037）. Conclusions　Remark-able results of practice skill training have been achieved. In the future， more chances for practice should be provided for trainees and contents of skill training should be designed according to the need of community health service， and more attention should be paid to the training project management.