Objective　To evaluate the application method and effect of standardized scenario simulation teaching based on Kirkpatrick model in vocational protection education for nursing students. Methods　A historical controlled trial study was designed. Practical nursing students enrolled in 2018-2019 and 2017-2018 were selected into the experimental group (n=203) and control group (n=196)

respectively. The experimental group adopted standardized scenario simulation teaching in the prevention and control education of needlestick injuries

and the control group adopted traditional classroom lecture. Using the Kirkpatrick model

the teaching effect of needlestick injuries protection for nursing students were compared between the 2 groups from such 4 levels as in reaction level

learning level

behavior level and results level. Results　There was no significant difference in the baseline data between the two groups in terms of age

gender

educational background and test scores of nursing professional knowledge as compared to that before practice. In reaction level: the nursing students' satisfaction of experimental group in teaching methods (t=25.149

P<0.001) and teaching environment (t=12.827

P<0.001) are higher than that of the control group

with statistical significance. In learning level: the test scores of needlestick injury knowledge in experimental group were significantly higher than those in control group (t=8.221

P<0.001). In behavior level: the level of needlestick injury protection behavior in the experimental group was significantly higher than that in the control group (t=9.250

P<0.001)

and the knowledge conversion rate in the experimental group was higher than that in the control group (t=6.054

P<0.001). In results level: the needlestick injuries incidence of experimental group was significantly lower than that of the control group ( ?字2=15.815

P<0.001)

the reported rate of needlestick injuries of experimental group was significantly lower than that of the control group ( ?字2=14.185

P<0.001). Conclusion　The implementation of standardized scenario simulation teaching can effectively improve the effectiveness of vocational protection learning and reduce the incidence of needlestick injuries.