Study of the expression of Ki-67 and apoptosis tested by TUNEL in the ER(-) breast cancer cell line

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Study of the expression of Ki-67 and apoptosis tested by TUNEL in the ER(-) breast cancer cell line

更新时间：2024-01-17

- Study of the expression of Ki-67 and apoptosis tested by TUNEL in the ER(-) breast cancer cell line
- Journal of Chongqing Medical University Issue 2, Pages: 151-153(2001)
- 作者机构：
  
  重庆医科大学临床学院普外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9
- Published：2001，
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[1]刘大川,姚榛祥.TUNEL法检测ER~((-))乳癌细胞凋亡和Ki-67表达的研究[J].重庆医科大学学报,2001(02):151-153.

Study of the expression of Ki-67 and apoptosis tested by TUNEL in the ER(-) breast cancer cell line. [J]. Journal of Chongqing Medical University (2):151-153(2001)
[1]刘大川,姚榛祥.TUNEL法检测ER~((-))乳癌细胞凋亡和Ki-67表达的研究[J].重庆医科大学学报,2001(02):151-153. DOI：

Study of the expression of Ki-67 and apoptosis tested by TUNEL in the ER(-) breast cancer cell line. [J]. Journal of Chongqing Medical University (2):151-153(2001) DOI：

摘要

目的：探讨雌激素受体阴性乳癌细胞中Ki-67与细胞凋亡之间的关系。方法：通过1

25（OH）2D3诱发雌激素受体阴性乳癌细胞株MDA-MB-435s凋亡，采用MTT法检测细胞增殖情况，TUNEL法检测细胞凋亡指数，免疫组化法检测Ki-67阳性表达指数，进行相关性分析。结果：在10-8mmol/L 1

25（OH）2D3诱发MDA-MB-435s凋亡过程中，肿瘤细胞凋亡指数与Ki-67阳性表达指数呈负相关。结论：对凋亡指数及Ki-67阳性表达指数的综合分析，可全面反映细胞凋亡和增殖的平衡状态，对于判断肿瘤细胞的增生具有参考价值。

Abstract

Objective: To test the relationship between Ki-67 index and apoptosis in the ER(-) breast cancer cell line. Methods: Apoptosis was induced by 1

25(OH)2D3 in the ER(-) breast cancer cell line. We compared the cell number by MTT

determined quantitatively apoptosis cells by TUNEL

and detected the expression of Ki-67 index immunohistochemically. Results: The expression of the Ki-67 index was decreased during apoptosis that induced by 10-8mmol/L 1

25(OH)2D3 in MDA-MB-435s cell line. there was negative correlation between the apoptosis and the expression of Ki-67 index. Conclusion: A comprehensive analysis of the apoptosis and Ki-67 index reflected the balance of proliferation and apoptosis.

关键词

125（OH）2D3雌激素受体阴性MDA-MB-435s凋亡Ki-67

Keywords

OH)2D3ER (-)MDA-MB-435sapoptosisKi-67

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