The effects of ultrasound on expression of integrins in mouse embryo and embryo implantation capability

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The effects of ultrasound on expression of integrins in mouse embryo and embryo implantation capability

更新时间：2024-01-17

- The effects of ultrasound on expression of integrins in mouse embryo and embryo implantation capability
- Journal of Chongqing Medical University Issue 1, Pages: 40-41(2001)
- 作者机构：
  
  1. 重庆医科大学第二临床学院妇产科,重庆,400010
  2. 重庆医科大学医学超声工程研究所,重庆,400046
- 作者简介：
- 基金信息：
- DOI：
  CLC： R445.1
- Published：2001，
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[1]李真,顾美礼,王智彪,胡凯,艾玲,杜永洪.超声波对小鼠胚胎表达整合素及其着床能力的影响[J].重庆医科大学学报,2001(01):40-41+44.

The effects of ultrasound on expression of integrins in mouse embryo and embryo implantation capability. [J]. Journal of Chongqing Medical University (1):40-41(2001)
[1]李真,顾美礼,王智彪,胡凯,艾玲,杜永洪.超声波对小鼠胚胎表达整合素及其着床能力的影响[J].重庆医科大学学报,2001(01):40-41+44. DOI：

The effects of ultrasound on expression of integrins in mouse embryo and embryo implantation capability. [J]. Journal of Chongqing Medical University (1):40-41(2001) DOI：

摘要

目的：研究超声波照射对小鼠胚胎表达整合素α1、α5、β1、 β3 的影响以及与其着床能力的关系。方法：取小鼠二细胞胚经超声波照射后体外培养至囊胚期，用免疫组化法检测其整合素α1、α5、β1、 β3 的表达及其着床能力的改变，并与未经照射的正常胚胎比较。结果：1、超声波照射组胚胎贴附率、滋养细胞外延生长率均显著低于正常胚胎。2、正常胚胎桑椹胚期即有α5、β1、β3 整合素表达，而无α1 表达；囊胚期α5、β1、β3 表达增强，仍无α1 表达；α1 只出现在外延生长的滋养细胞。3、超声波照射组胚胎β3 整合素的表达明显弱于正常组，且未见α1 表达；而α5、 β1 的表达两组间无差异。结论：超声波照射使胚胎着床能力明显降低，可能与其影响胚胎细胞α1、β3 整合素的正常表达有关。

Abstract

Objective: To study the effects of ultrasound on expression of integrinα1

α5

β1

β3 in mouse embryos and its implantation capability. Methods: Two cells mouse embryos irradiated by ultrasound were cultured into blastocysts. Results: 1. The attachment rate and the trophoblasts outgrowth rate in irradiated embryos were lower significantly than that in the normals. 2. The expression of subunits α5

β1

β3 was detected in normal morulas and increased in blastocysts. The expression of subunit α1 was not detected in morulas or blastocysts

but detected only in outgrowing trophocytes. 3. Expression of integrin β3 in irradiated group embryos was weaker than that of normal group. There was no expression of integrin α1 both in morulas and blastocysts in irradiated group and no difference in the expression of subunits α5 and β1 between these two groups. Conclusion: Ultrasound may damage the embryo implantation capability by impacting the nomal expression of α1

β3 integrins in embryo cells.

关键词

超声波胚胎着床整合素

Keywords

UltrasoundEmbryosImplantationIntegrins

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Related Institution

Department of Obstetrics and Gynecology,The First Affiliated Hospital of Chongqing Medical University,China-Canada-New Zealand Joint Laboratory of Maternal and Fetal Medicine,Chongqing Medical University

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重庆医科大学生殖医学系

Department of Reproductive Medical Science,Chongqing Medical University

重庆医科大学生殖医学系遗传优生教研室

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