Preparation and identification of duck hepatitis B virus (DHBV) surface antigen from infected duck serum

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Preparation and identification of duck hepatitis B virus (DHBV) surface antigen from infected duck serum

更新时间：2024-01-17

- Preparation and identification of duck hepatitis B virus (DHBV) surface antigen from infected duck serum
- Journal of Chongqing Medical University Issue 1, Pages: 14-16(2001)
- 作者机构：
  
  重庆医科大学病毒性肝炎研究所,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC： R373.2
- Published：2001，
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[1]唐霓,黄爱龙,郭树华,张定凤.鸭乙型肝炎病毒表面抗原的纯化及应用[J].重庆医科大学学报,2001(01):14-16.

Preparation and identification of duck hepatitis B virus (DHBV) surface antigen from infected duck serum. [J]. Journal of Chongqing Medical University (1):14-16(2001)
[1]唐霓,黄爱龙,郭树华,张定凤.鸭乙型肝炎病毒表面抗原的纯化及应用[J].重庆医科大学学报,2001(01):14-16. DOI：

Preparation and identification of duck hepatitis B virus (DHBV) surface antigen from infected duck serum. [J]. Journal of Chongqing Medical University (1):14-16(2001) DOI：

摘要

目的：从鸭乙型肝炎病毒（DHBV）感染鸭血清中纯化表面抗原（DHBsAg），初步应用于病毒感染后复制的研究。方法：用蔗糖密度梯度离心纯化病毒表面抗原，经Bradford法定量血清中该蛋白含量；建立DHBsAg ELISA检测方法，并与DHBV DNA斑点杂交相比较。结果：纯化DHBsAg 经ELISA检测OD490nm≥1.0，Western杂交显示主要肽分子量为17KDa和35KDa；Bradford法定量血清蛋白含量为2.85～5.10μg/ml

DHBsAg ELISA检测100份血清标本阳性数为84例

斑点杂交检测阳性数为88例

两者吻合率为94%。结论：DHBsAg 的制备及ELISA方法的建立，为进一步研究病毒感染后复制及体内免疫应答状况奠定了基础。

Abstract

Objective: Preparation of DHBsAg from DHBV infected ducks and its application for the study of virus replication. Methods : The positive serum were layered over a step gradient of 20% and 66% sucrose

DHBsAg were harvested at the interface between 20% and 66% sucrose

and then analyzed by Bradford method. Meanwhile

DHBsAg ELISA and DHBV DNA dot blot hybridization assayed 100 serum samples. Results : Purified antigen were detected OD490nm≥1.0 by DHBsAg ELISA assay and quantified 2.85~5.10μg/ml by Bradford method. Both 35KDa and 17KDa pres/s bands were present in DHBV-infected serum. DHBsAg assayed 84 positive in 100 serum samples

while DHBV DNA dot blot hybridization detected 88 positive

showing good accordance. Conclusion: Preparation of DHBsAg from DHBV-infected ducks and application of DHBsAg ELISA assay will be helpful to the study of kinetics of DHBV and immune response of ducks to the virus.

关键词

鸭乙型肝炎病毒表面抗原Western 杂交

Keywords

Duck hepatitis B virus surface antigenWestern blot

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