Influences of donor blood transfusion decreasing expressions of IL-2,  TNF-α for pancreas allograft in rats

您当前的位置：

首页 >

文章列表页 >

Influences of donor blood transfusion decreasing expressions of IL-2, TNF-α for pancreas allograft in rats

更新时间：2024-01-17

- Influences of donor blood transfusion decreasing expressions of IL-2, TNF-α for pancreas allograft in rats
- Journal of Chongqing Medical University Issue 1, Pages: 17-19(2001)
- 作者机构：
  
  重庆医科大学临床学院器官移植研究室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R617
- Published：2001，
扫描看全文
[1]孙治君,姚榛祥.输入供体血对大鼠移植胰腺白介素-2和TNF-α的影响[J].重庆医科大学学报,2001(01):17-19.

Influences of donor blood transfusion decreasing expressions of IL-2, TNF-α for pancreas allograft in rats. [J]. Journal of Chongqing Medical University (1):17-19(2001)
[1]孙治君,姚榛祥.输入供体血对大鼠移植胰腺白介素-2和TNF-α的影响[J].重庆医科大学学报,2001(01):17-19. DOI：

Influences of donor blood transfusion decreasing expressions of IL-2, TNF-α for pancreas allograft in rats. [J]. Journal of Chongqing Medical University (1):17-19(2001) DOI：

摘要

目的：了解输入供体血延长大鼠移植胰腺功能存活的机制。方法：取封闭群健康wistar大鼠作为供受体。制成糖尿病模型的受体大鼠分为对照和输入供体血二组。采用胰管开放全胰腺腹腔内移植。术后7天处死动物，取移植胰腺作HE染色和胰岛gomori改良醛复红染色，碱性磷酸酶桥联酶标法检测淋巴细胞和细胞因子。结果：输入供体血组的移植胰腺腺泡和胰岛结构正常，CD+4T细胞显著增高。对照组胰腺腺泡和胰岛结构破坏，CD+8T细胞，IL-2，TNF-α显著增高。对照组CD+3T细胞、NK细胞均比输入供体血组高，但无显著差异。结论：输入供体血延长大鼠移植胰腺功能存活与IL-2，TNF-α降低有关。

Abstract

Objective: To study potential mechanisms of DST(donor-specific transfusion) prolonging pancreas allograft survival in the rat model. Methods: Outbred wistar male rats were used as blood and pancreas donor and diabetic recipients. Diabetic recipients were divided into two groups: control and DST group. Whole pancreas transplantation in abdomen were performed with pancreatic ductopen. Two groups of rats were killed for 7 days after transplantation. Grafted pancreas were stained with Hematoxylin and eosin stain method. Islets were stained with Gomori modified aldehyde fuchsin stain method. Alkaline phosphatase enzyme immunoassay method was used to detect lymphocyte and cytokines in allografts. Results: Analysis of grafts showed that both control and DST rats had lymphocytes infiltrates

higher percentage of CD+4 T cells in DST group and higher percentage of CD+8 T cells in control group. There was no evidence of pancreas and islet injury in DST rats. Grafts in control rats were characterized by pancreas and islet injury and expression of IL-2

TNF-α. Conclusion: These results indicated that DST prolonging pancreas allograft survival was associated with decreasing expression of IL-2

TNF-α in grafts.

关键词

大鼠供体血胰腺移植IL-2TNF-α

Keywords

ratsDSTPancreas transplantIL-2TNF-α

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

THE EFFECT OF INFARCT EXPANSION ON LEFT VENTRICULAR FUNCTION IN RATS

THE TRANSFERABLE AND IMMUNOLOGICAL STUDIES ON SHOCK TOXIN

THE STUDY OF LYMPHOKINE ACTIVITIES IN PRIMARY HUMORAL IMMUNODEFICIENCIES

The model of introabdominal transplantation ofwhole pancreas graft in rats

Effects of recombinant BCG-Sj26GST vaccine of schistome on the expression of TNF-α mRNA during the formation of egg granulomas in the liver of mice

Related Author

Department of Pathophysiology: Du Xiaojun

Gong Xuling

et al

吴崇荣

刘银君

郑明祺

Li Chengrong

Yang xiqiang Hans D .Ochs

Related Institution

重庆医学院病理生理教研室

Department of Pharmacology

重庆医科大学儿科医院美国华盛顿大学医学院儿科免疫实验室教授重庆医科大学名誉教授

美国华盛顿大学医学院儿科免疫实验室教授重庆医科大学名誉教授

Department of pediatrics. Children’s Hospital

⁰