Effect of antisense oligodeoxynucleotide targeting against telomerase on proliferation of laryngeal carcinoma cells

PU Yiping; et al

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Effect of antisense oligodeoxynucleotide targeting against telomerase on proliferation of laryngeal carcinoma cells

更新时间：2024-01-17

- Effect of antisense oligodeoxynucleotide targeting against telomerase on proliferation of laryngeal carcinoma cells
- Journal of Chongqing Medical University Issue 2, Pages: 160-162(2002)
- 作者机构：
  
  1. 重庆医科大学第二临床学院耳鼻咽喉科
  2. 贵州安顺市第二人民医院五官科
  3. 重庆医科大学第二临床学院耳鼻咽喉科,重庆,400010
  4. ,安顺,561000
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.65
- Published：2002，
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[1]蒲一平,刘勇,吴家廉,曹前.反义寡核苷酸抑制端粒酶活性对喉癌细胞Hep-2增殖的影响[J].重庆医科大学学报,2002(02):160-162.

PU Yiping, et al. Effect of antisense oligodeoxynucleotide targeting against telomerase on proliferation of laryngeal carcinoma cells. [J]. Journal of Chongqing Medical University (2):160-162(2002)
[1]蒲一平,刘勇,吴家廉,曹前.反义寡核苷酸抑制端粒酶活性对喉癌细胞Hep-2增殖的影响[J].重庆医科大学学报,2002(02):160-162. DOI：

PU Yiping, et al. Effect of antisense oligodeoxynucleotide targeting against telomerase on proliferation of laryngeal carcinoma cells. [J]. Journal of Chongqing Medical University (2):160-162(2002) DOI：

摘要

目的:检测端粒酶的反义寡核苷酸对喉癌细胞的影响。Hep-2方法:采用法及平板集落形成实验检测靶向于端MTT粒酶的反义寡核苷酸对喉癌细胞株增殖的抑制作用。用法检测反义寡核苷酸对喉癌细胞株细胞内端粒Hep-2 PCR-ELISAHep-2酶活性的影响。结果:反义寡核苷酸明显抑制了喉癌细胞的增殖

此作用有剂量、时间依赖性。反义寡核苷酸可抑制喉Hep-2癌细胞端粒酶活性

此作用有浓度依赖性。结论:靶向于端粒酶的反义寡核苷酸能抑制喉癌细胞的增殖。Hep-2

Abstract

Objective: To investigate the effect of the antisense oligodeoxynucleotide (AS-ODN) targeting against telomerase on laryngeal carcinoma cells Hep-2 strain in vitro. Methods: MTT test and plat clone forming test were used to measure the proliferation inhibition of the AS-ODN targeting against telomerase on Hep-2 cell strain. Telomerase activity of Hep-2 cell strain was analysed by PCR-ELISA technique. Results: The AS-ODN inhibited Hep-2 cell strain obviously with dose dependence and time dependence. The AS-ODN inhibited telomerase activity of Hep-2 cell strain with density dependence. Conclusion: The AS-ODN targeting against telomerase could inhibit the proliferation of Hep-2 cell strain.

关键词

喉癌反义寡核苷酸端粒酶

Keywords

laryngeal carcinomaAntisense oligodeoxynucleotidetelomerase

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