Experimental study on expression of GM-CSF, IL-3 from fetal thymocytes and splenocytes

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Experimental study on expression of GM-CSF, IL-3 from fetal thymocytes and splenocytes

更新时间：2024-01-17

- Experimental study on expression of GM-CSF, IL-3 from fetal thymocytes and splenocytes
- Journal of Chongqing Medical University Issue 4, Pages: 373-375(2002)
- 作者机构：
  
  重庆医科大学基础医学研究所,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2002，
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[1]郑敏,王亚平,姜蓉,吴宏.当归多糖诱导胎儿胸腺细胞、脾细胞表达GM-CSF、IL-3的实验研究[J].重庆医科大学学报,2002(04):373-375.

Experimental study on expression of GM-CSF, IL-3 from fetal thymocytes and splenocytes. [J]. Journal of Chongqing Medical University (4):373-375(2002)
[1]郑敏,王亚平,姜蓉,吴宏.当归多糖诱导胎儿胸腺细胞、脾细胞表达GM-CSF、IL-3的实验研究[J].重庆医科大学学报,2002(04):373-375. DOI：

Experimental study on expression of GM-CSF, IL-3 from fetal thymocytes and splenocytes. [J]. Journal of Chongqing Medical University (4):373-375(2002) DOI：

摘要

目的：研究“补血活血”要药当归的主要有效成分-当归多糖（APS）对胎儿胸腺细胞、脾细胞表达造血生长因子GM-CSF、IL-3的影响。方法：采用造血祖细胞体外培养、造血生长因子生物学活性检测、免疫细胞化学、核酸探针原位杂交等实验技术。结果：APS诱导的胸腺细胞、脾细胞培养上清液可促进早期造血祖细胞CFU-Mix增殖分化；APS诱导可促进胸腺细胞、脾细胞表达GM-CSF、IL-3蛋白及mRNA。结论：APS可促进淋巴细胞表达造血生长因子GM-CSF、IL-3，进而促进人早期造血细胞发生。

Abstract

Objective: To explore the effect of Angelica polysaccharide (APS) on the expression of GM-CSF and IL-3 from fetal thymocytes (FTC) and splenocytes (FSC). Methods: Techniques of hematopoietic progenitor cell culture in vitro

biological assay of hematopoietic growth factor (HGF)

immunocytochemistry and in situ hybridization were used. Results: The conditioned media of FTC and FSC induced by APS can markedly promote the proliferation and differentiation of early multipotential progenitor cell (CFU-Mix)；the expression of GM-CSF

IL-3 protein in FTC and FSC induced by APS were much stronger than that of the control; expression level of GM-CSF and IL-3mRNA showed similar tendency. Conclusion: APS promotes the expression of GM-CSF\IL-3 of FTC andFSC

then proceeds to promote early human hematopoiesis.

关键词

当归多糖淋巴细胞多向性造血祖细胞造血生长因子造血调控

Keywords

Angelica polysaccharidelymphocyteMultipotential progenitor cellHematopoietic growth factorHematopoietic regulation

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Related Institution

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