Study on the relationship between expression of p-glycoprotion in peripheral blood mononuclear cell and multidrug resistance of patients with cancer

JIN Xianqing; et al

您当前的位置：

首页 >

文章列表页 >

Study on the relationship between expression of p-glycoprotion in peripheral blood mononuclear cell and multidrug resistance of patients with cancer

更新时间：2024-01-17

- Study on the relationship between expression of p-glycoprotion in peripheral blood mononuclear cell and multidrug resistance of patients with cancer
- Journal of Chongqing Medical University Issue 4, Pages: 376-378(2002)
- 作者机构：
  
  1. 重庆医科大学儿科学院外科
  2. 重庆医科大学儿科学院外科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：
  CLC： R730.5
- Published：2002，
扫描看全文
[1]金先庆,张喜平,王珊,许嘉陵.恶性肿瘤患者外周血细胞P-糖蛋白表达与肿瘤多药耐药相关性研究[J].重庆医科大学学报,2002(04):376-378+382.

JIN Xianqing, et al. Study on the relationship between expression of p-glycoprotion in peripheral blood mononuclear cell and multidrug resistance of patients with cancer. [J]. Journal of Chongqing Medical University (4):376-378(2002)
[1]金先庆,张喜平,王珊,许嘉陵.恶性肿瘤患者外周血细胞P-糖蛋白表达与肿瘤多药耐药相关性研究[J].重庆医科大学学报,2002(04):376-378+382. DOI：

JIN Xianqing, et al. Study on the relationship between expression of p-glycoprotion in peripheral blood mononuclear cell and multidrug resistance of patients with cancer. [J]. Journal of Chongqing Medical University (4):376-378(2002) DOI：

摘要

目的：本研究通过外周血单个核细胞（PBMC）、CD4+、CD8+细胞P-糖蛋白的表达与肿瘤组织P-糖蛋白表达的相关性研究以及PBMC、CD4+、CD8+细胞P-糖蛋白在化疗过程中的动态变化的观察，寻找动态诊断MDR的新途径。方法：本组共51例肿瘤病人，23例采用免疫组化检测PBMC及肿瘤组织的P-糖蛋白，28例用流式细胞术测定PBMC、CD4+、CD8+ 细胞P-糖蛋白，用免疫组化测定相应的肿瘤组织的P-糖蛋白。结果：①肿瘤患者PBMC、CD4+、CD8+细胞P-糖蛋白表达较正常人高（P＜ 0.05）。②PBMC、CD4+、CD8+细胞P-糖蛋白表达水平与肿瘤组织P-糖蛋白呈显著的相关性（r =0.419

P<0.05）；③PBMC、CD4+P-糖蛋白化疗后较化疗前显著增加，可通过PBMC、CD4+P-糖蛋白的变化动态反映肿瘤细胞的变化。④用FCM检测PBMC P-糖蛋白与IC法比较，FCM方法简单，精确度高，更适合血细胞的检测。结论：PBMC、CD4+、CD8+P-糖蛋白含量与肿瘤P-糖蛋白表达有明显相关性。通过PBMC、CD4+、CD8+P-糖蛋白的检测，为临床判断肿瘤耐药提供了一条简便的途径。

Abstract

Objective: At present

the detection of multidrug resistance depends on resected specimen but it can not be investigated in patients who were not operated on or got a long term chemotherapeutic treatment after operation. In this study

the relationship between p-glycoprotein expression in PBMC，CD 4+，CD8+ and tumor cells has been investigated. In order to obtain a new determination method for multidrug resistance

the changes of p-glycoprotein expression in PBMC，CD 4+，CD 8+ during chemotherapeutic stage has been analysed. Methods: The expression of p-glycoprotein in PBMC，CD 4+，CD8+ and tumor cells has been detected by means of immunohistochemestry assay in 23 cases

flow cytometry and immunohistochemestry assay in 28 cases to identify the relationship of p-glycoprotein between PBMC，CD 4+，CD8+ and tumor cells. P-glycoprotein in PBMC，CD 4+ and CD8+ was measured in 17 of 28 patients with chemotherapeutic treatment for six months. Results: ①The p-glycoprotein expression in PBMC，CD4+，CD8+ of patient with malignant tumor was higher than the control. ②A significance relationship of p-glycoprotein had been found between PBMC，CD 4+，CD8+ and tumor cells. ③ The p-glycoprotein expression in PBMC，CD 4+，CD 8+ increased significantly in chemotherapeutic group as compared with non-chemotherapeutic group. ④Flow cytometry assay was more sensitive than immunohistochemestry in detecting p-glycoprotein expression in PBMC，CD 4+，CD8+ . Conclusion:The detection of p-glycoprotein in PBMC，CD 4+，CD8+ provides a new method in the MDR determination. A relationship of p-glycoprotein has been found between PBMC，CD 4+，CD8+ and tumor cells.

关键词

多药耐药外周血单个核细胞肿瘤免疫组化流式细胞术

Keywords

Peripheral blood mononuclear cellMultidrug resistancetumorFlow cytometryImmunohisto-chemistry

references

Views

128

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Advances in metabolic enzymes in the hexosamine biosynthetic pathway and OGT-mediated O-GlcNAc modification in tumors

Advances in and reflections on bacteria-mediated oncology therapy

Masses in maxillofacial region of 859 children:a retrospective analysis

Research progresses of the relationship between dioxygenases in kynurenine pathway and tumor

DETERMINATION OF ANTICANCER DRUG SENSITIVITY TEST BY THE MTT COLO RIMETRIC ASSAY FOR HUMAN SOLID TUMORS

Related Author

Yang Jiayao

Tang Ni

Wang Kai

Zining Wang

Ying Zhu

Yang Luo

Wei Gu

Li Liqi

Related Institution

Key Laboratory of Molecular Biology of Infectious Diseases，Ministry of Education，Chongqing Medical University

Center for Intelligent Testing and Molecular Medicine， School of Medicine， Chongqing University

Department of Stomatology,the Children’s Hospital,Chongqing Medical University

Department of Gastrointestinal Surgery,the Second Affiliated Hospital of Nanchang University

the Second Affiliated Medical College of Nanchang University

⁰