Effect of carthamus tinctorius L.injection on caspase-3,bcl-2 expression related to renal ischemia/reperfusion injury in rats

GAO Fei; et al

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Effect of carthamus tinctorius L.injection on caspase-3,bcl-2 expression related to renal ischemia/reperfusion injury in rats

更新时间：2024-01-17

- Effect of carthamus tinctorius L.injection on caspase-3,bcl-2 expression related to renal ischemia/reperfusion injury in rats
- Journal of Chongqing Medical University Issue 4, Pages: 532-536(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院泌尿外科
  2. 重庆医科大学检验系
  3. 重庆医科大学基础医学院解剖教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285
- Published：2006，
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[1]高飞,吴小候,罗春丽,杨美.红花注射液对大鼠肾缺血再灌注损伤Caspase-3和bcl-2表达的影响[J].重庆医科大学学报,2006(04):532-536.

GAO Fei, et al. Effect of carthamus tinctorius L.injection on caspase-3,bcl-2 expression related to renal ischemia/reperfusion injury in rats. [J]. Journal of Chongqing Medical University (4):532-536(2006)
[1]高飞,吴小候,罗春丽,杨美.红花注射液对大鼠肾缺血再灌注损伤Caspase-3和bcl-2表达的影响[J].重庆医科大学学报,2006(04):532-536. DOI：

GAO Fei, et al. Effect of carthamus tinctorius L.injection on caspase-3,bcl-2 expression related to renal ischemia/reperfusion injury in rats. [J]. Journal of Chongqing Medical University (4):532-536(2006) DOI：

摘要

目的:探讨红花注射液对大鼠肾缺血再灌注损伤凋亡相关基因Caspase-3和bcl-2表达的影响。方法:将75只SD大鼠随机分为正常对照组、缺血再灌注组和治疗组。建立肾缺血再灌注损伤模型

检测肌酐和血尿渗透压值评价肾功能变化

TUNEL法检测凋亡率

免疫组化检测Caspase-3和bcl-2蛋白表达。结果:与正常对照组相比

缺血再灌注组肾功能明显减退

凋亡率显著增加、Caspase-3和bcl-2表达明显增强（P<0.01）;运用红花注射液治疗后

与缺血再灌注组相比

治疗组肾功能明显改善

凋亡率显著减少、Caspase-3的表达明显减弱而bcl-2表达明显增强（P<0.01）。结论:在肾缺血再灌注损伤过程中

红花注射液具有抑制Caspase-3表达和促进bcl-2表达的作用。

Abstract

Objective: To investigate the effect of carthamus tinctorius injection on expression of caspase-3 and bcl-2 after renal ischemia/reperfusion injury.Methods: Seventy-five SD rats were randomly divided into three groups(n=25

in each):normal control group

ischemia/reperfusion group and treatment group.After the renal ischemia/reperfusion injury model was established

the serum creatinine、the urine osmotic pressure and the blood osmotic pressure were measured to evaluate the change of renal function;the apoptosis rate in ischemia/reperfusion renal tissure were measured by TUNEL method;the caspase-3 and bcl-2 concentration were measured by immunohistochemistry.Results:The renal function got worse

and the apoptosis rate

bcl-2 and caspase-3 concentration were significantly higher in ischemia/reperfusion group than those in normal control group.As compared with ischemia/reperfusion group

the renal function was improved

the apoptosis rate decreased

bcl-2 expression increased and caspase-3 expression decreased significantly in treatment group.Conclusion:Carthamus tinctorius injection has significant effect on the up-regulation of bcl-2 and down-regulation of caspase-3 expression in renal ischemia/reperfusion injury.

关键词

红花注射液缺血再灌注凋亡Caspase-3Bcl-2

Keywords

Carthamus tinctorius injectionischemia/reperfusionapoptosisCaspase-3Bcl-2

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