Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor

LI Wei; et al

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Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor

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- Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor
- Journal of Chongqing Medical University Issue 2, Pages: 227-230(2006)
- 作者机构：
  
  1. 成都市妇幼保健院妇产科
  2. 重庆医科大学附属第一医院妇产科
  3. 成都市妇幼保健院妇产科,成都,610031
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R318.0
- Published：2006，
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[1]李玮,漆洪波,陈蓉,周辉.人羊膜上皮细胞在纤维蛋白支架和细胞生长因子作用下的研究[J].重庆医科大学学报,2006(02):227-230.

LI Wei, et al. Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor. [J]. Journal of Chongqing Medical University (2):227-230(2006)
[1]李玮,漆洪波,陈蓉,周辉.人羊膜上皮细胞在纤维蛋白支架和细胞生长因子作用下的研究[J].重庆医科大学学报,2006(02):227-230. DOI：

LI Wei, et al. Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor. [J]. Journal of Chongqing Medical University (2):227-230(2006) DOI：

摘要

目的:以纤维蛋白凝块为支架构建人羊膜上皮细胞（HAEC）生长模型

观察表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）、转化生长因子β1（TGF-b1）对HAEC生长的影响。方法:在体外构建的纤维蛋白支架上培养HAEC

采用倒置显微镜、Giemsa染色和扫描电子显微镜观察HAEC的生长情况。采用TUNEL法检测EGF、bFGF和TGF-β1对纤维蛋白支架上HAEC凋亡的影响。结果:HAEC在构建的纤维蛋白支架上生长。EGF组和bFGF组HAEC凋亡较对照组明显减少（P<0.05）

TGF-β1组HAEC凋亡较对照组明显增加（P<0.05）。结论:HAEC能在构建的纤维蛋白支架上良好生长

EGF、bFGF可抑制HAEC凋亡

TGF-β1则促进HAEC凋亡。

Abstract

Objective: To establish the model of cultured human amniotic epithelial cells(HAECs) on the fibrin clot in order to observe the effect of EGF

bFGF and TGF-β1 on HAEC.Methods:The cultivated HAECs on the formulated fibrin clot were examined by phase contrast microscopy

Giemsa staining and scanning electron microscopy.The effect of growth factor(EGF

bFGF

and TGF-β1) on the apoptosis of HAECs was measured by TUNEL.Results: HAECs generated on the formulated fibrin clot in vitro.HAECs exposed to EGF or bFGF showed a lower rate of apoptosis than the control group(P<0.05)

however

HAECs exposed to TGF-β1 showed an opposite effect(P<0.05).Conclusion: HAECs generate well on the formulated fibrin clot in vitro.Exposure to EGF or bFGF decreases the apoptosis rate of HAEC

however

exposure to TGF-β1 increases the apoptosis.

关键词

人羊膜上皮细胞纤维蛋白生长因子

Keywords

HAECfibrin clotgrowth factor

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刘慧

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ZHANG Lei

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LI Weiwei

Related Institution

Department of Anesthesia,West China Hospital,Sichuan University

Department of Biochemistry

Department of Laboratory Medicine，the Third Affiliated Hospital，Liaoning Medical College

Department of Ophthalmolog，The First Affiliated Hospital of Chongqing Medical University

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