Expression of AQP1 in ocular tissues of rat

LI Jiawen; et al

您当前的位置：

首页 >

文章列表页 >

Expression of AQP1 in ocular tissues of rat

更新时间：2024-01-17

- Expression of AQP1 in ocular tissues of rat
- Journal of Chongqing Medical University Issue 2, Pages: 223-226(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院眼科
  2. 重庆医科大学附属第一医院眼科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q954.6
- Published：2006，
扫描看全文
[1]李嘉文,李平华,张黎.大鼠眼内组织水通道蛋白1的表达[J].重庆医科大学学报,2006(02):223-226.

LI Jiawen, et al. Expression of AQP1 in ocular tissues of rat. [J]. Journal of Chongqing Medical University (2):223-226(2006)
[1]李嘉文,李平华,张黎.大鼠眼内组织水通道蛋白1的表达[J].重庆医科大学学报,2006(02):223-226. DOI：

LI Jiawen, et al. Expression of AQP1 in ocular tissues of rat. [J]. Journal of Chongqing Medical University (2):223-226(2006) DOI：

摘要

目的:观察水通道蛋白1（AQP1）在正常大鼠眼内组织的表达部位。方法:雄性Wistar大鼠断头放血取出眼球

4%多聚甲醛固定

石蜡包埋。做矢状面5μm连续切片

每个标本切3张

分别用于AQP1免疫组化染色、阴性对照和HE染色

用已知有AQP1表达的大鼠肾脏肾小管切片为阳性对照。结果:光镜下观察AQP1免疫组化染色切片

细胞中出现棕色粗大颗粒为阳性

反之为阴性。大鼠眼内组织AQP1阳性表达部位有:（1）角膜内皮细胞;（2）虹膜上皮细胞;（3）晶状体上皮细胞;（4）睫状体非色素上皮（NPE）细胞;（5）视网膜神经节细胞层、内核层;（6）视神经胶质细胞。结论:AQP1在眼内与水代谢有关的多个部位呈阳性表达。

Abstract

Objective:To detect the expression of aquaporin-1(AQP1) in ocular tissues of normal rats.Methods:The eyeballs were enucleated from the male Wistar rats

fixed with 4% formaldehydum polymerisatum and embeded in paraffin.Then

5μm serial section was made on the sagittal plane.Each sample was cut into 3 pieces for AQP1 HE staining and immunohistochemical staining

the latter of which included negative control and positive control.The immunohistochemical staining of renal tubule

known as positive expression of AQP1

was used as positive control.Results:Under the light microscope

the brown cellular granules indicated positive-AQP1 cells.AQP1 was expressed in corneal endothelial cells

iris epithelial cells

crystalline lens epithelial cells

ciliary body non-pigment epithelial(NPE) cells

ganglion cell layer and inner nuclear layer of retina

optic nerve glial cells.Conclusions: AQP1 is expressed in many places of ocular tissues related to the water metabolism.

关键词

水通道蛋白1免疫组织化学眼组织

Keywords

Aquaporin-1immunohistochemistryOcular tissues

references

Views

123

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression and significance of FOXP3 in colon cancer cells

Expression of HIF-1α and Sema4D in pancreatic carcinoma and its clinical significance

HISTOCHEMICAL AND IMMUNOHISTOCHEMI- CAL STUDY OF MAMMAKY PAGET’S DISEASE

THE EXPRESSION AND SIGMFICANCE OF HMB45,S-100 PROTEIN AND VIMENTIN IN PRIMARY MALIGNANT MELANOMA

Histopathologic examination of uterine smooth muscle tumors

Related Author

Qiu Lei

Shao Hua

Miao Yongchang

Jia Rujiang

Yin Qingchen

Wang Xiuchao

Hou Liyan

Xu Chao

Related Institution

Department of Gastrointestinal Surgery,The Second People’s Hospital of Lianyungang City

Department of General Surgery,Handan Central Hospital

Department of Pancreatic Oncology,Tianjin Medical University Cancer Institute and Hospital

重庆医科大学基础医学院病理教研室

重庆医科大学临床学院眼科

⁰