Determination of selenium in serum with Luminol-H<sub>2</sub>O<sub>2</sub>-KMnO4 chemiluminescence system

YI Gang; et al

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Determination of selenium in serum with Luminol-H₂O₂-KMnO4 chemiluminescence system

更新时间：2024-01-17

- Determination of selenium in serum with Luminol-H₂O₂-KMnO4 chemiluminescence system
- Journal of Chongqing Medical University Issue 1, Pages: 119-120(2006)
- 作者机构：
  
  1. 重庆医科大学医学检验系重庆医科大学临床检验诊断学实验室
  2. 重庆医科大学医学检验系重庆医科大学临床检验诊断学实验室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R446.1
- Published：2006，
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[1]易钢,颜玉蓉,钟梁,涂植光.静态化学发光淬灭分析法测定血清硒[J].重庆医科大学学报,2006(01):119-120+128.

YI Gang, et al. Determination of selenium in serum with Luminol-H₂O₂-KMnO4 chemiluminescence system. [J]. Journal of Chongqing Medical University (1):119-120(2006)
[1]易钢,颜玉蓉,钟梁,涂植光.静态化学发光淬灭分析法测定血清硒[J].重庆医科大学学报,2006(01):119-120+128. DOI：

YI Gang, et al. Determination of selenium in serum with Luminol-H₂O₂-KMnO4 chemiluminescence system. [J]. Journal of Chongqing Medical University (1):119-120(2006) DOI：

摘要

目的:探讨硒对鲁米诺（Luminol）-过氧化氢-高锰酸钾发光体系的阻化作用并实现血清硒的化学发光测定。方法:采用GD-1型液相化学发光仪测定鲁米诺-过氧化氢-高锰酸钾反应体系的化学发光强度

建立化学发光强度与溶液中硒浓度的标准曲线

从而实现血清硒的静态化学发光淬灭分析测定。结果:本方法的检出限为6.5×10-4μg/ml

线性范围为1.5×10-3～6.5×10-1μg/ml;平均回收率为94.21%

变异系数为2.97%。结论:本方法是一种简便、准确的血清硒检测方法

对Mg2+、Cu2+等多种离子的干扰实验表明方法具有较好的选择性。

Abstract

Objective: To study the quenchable effect of Selenium to Luminol-H2O2-KMnO4 Chemiluminescence system and to establish a method for the determination of Selenium in serum.Methods: Chemiluminescence system was used to determine the Chemiluminogenic strength of Luminol-H2O2-KMnO4 and the detection of Selenium in serum was realized.Results:A detection limit with 6.5×10-4μg/ml and a 1.5×10-3～6.5×10-1μg/ml linear range were obtained.The average recovery fraction was 94.21% and the relative standard deviation was 2.97%.Conclusion: A satisfactory result for the determination of Selenium in serum by this method is obtained.

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鲁米诺化学发光硒

Keywords

LuminolChemiluminescenceSelenium

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YI Gang

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Key Laboratory of Laboratory Medical Diagnostics,Ministry of Education,Chongqing Medical University

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Determination of selenium in serum with Luminol-H2O2-KMnO4 chemiluminescence system

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Abstract

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Determination of selenium in serum with Luminol-H₂O₂-KMnO4 chemiluminescence system